Crystal structure of staphylococcus aureus autolysin e, method of producing the crystal and its use in screening methods

ABSTRACT

The invention concerns the determination and evaluation of the crystal structure of autolysin E (AtlE) of  Staphylococcus aureus  ( S. aureus ), or a crystallizable fragment of AtlE, a method for producing a crystal of AtlE and the respective crystallization kit, and its use in a method for screening an inhibitor of the N-acetylglucosaminidase activity of AtlE, for obtaining atomic spatial relationship data, and for identifying a binding compound of AtlE, e.g. by in silico screening.

SUMMARY

The present invention concerns the determination and evaluation of the crystal structure of Autolysin E (AtlE) of Staphylococcus aureus (S. aureus), or a crystallizable fragment of AtlE, a method for producing a crystal of AtlE and the respective crystallization kit, and its use in a method for screening an inhibitor of the N-acetylglucosaminidase activity of AtlE, for obtaining atomic spatial relationship data, and for identifying a binding compound of AtlE and other GH73 family glucosaminidases from S. aureus, e.g. by in silico screening.

BACKGROUND

A characteristic structural component of the cell wall of bacteria is peptidoglycan. The peptidoglycan cell wall surrounds the bacterial cell, provides structural support, and shields the bacterial membrane against osmotic rapture. It is composed of alternating N-acetylglucosamine (NAG) and N-acetylmuramic acid (NAM) residues connected with β(1,4)-glycosidic bonds [Boneca et al., 2000] and cross-linked with short polypeptide chains. Bacterial growth, division, colonization, and biofilm formation heavily relies on the ability of the cells to remodel their cell wall. Therefore, many antibiotics, such as penicillin, target the biosynthesis of the cell wall. Due to the increasing resistance of bacteria to antibiotics, it is important to find alternative targets for the treatment of bacterial infections, e.g. enzymes involved in the cell wall degradation. Cell wall degradation is performed by peptidoglycan hydrolases, also known as autolysins [Smith et al, 2000]. Among them are glycosidases which hydrolyze glycosidic bonds in NAM-NAG polymers. There exists two major types of glycosidases, N-acetylglucosaminidases and N-acetylmuramidases (lysozyme-like proteins), which cleave the β(1,4)-glycosidic bonds between NAG-NAM, and NAM-NAG residues, respectively [Vollmer et al., 2008]. As glucosaminidases and muramidases act on the same substrate, it is generally useful to gain insight into their biochemical properties and reveal features which enable them to perform the two distinct functions.

Staphylococci represent a large group of bacteria which inhabit humans and can cause severe infections to people with weak or compromised immune system. Among them is Staphylococcus aureus (S. aureus) which is responsible for most of hospital acquired infections [Vincent et al., 2009]. S. aureus is a Gram-positive bacterial pathogen that is responsible for severe medical conditions in humans, including bacteremia, endocarditis, metastatic infections, sepsis and toxic shock syndrome [Lowy, 1998], osteomyelitis [Varonne et al., 2011]. S. aureus possesses the capability to form biofilms during the progress of infection which is of advantage of the bacteria. Biofilms offer protection of the cells against antibiotics and the host immune response which leads to the development of a long and persisting chronic disease [Archer et al. 2011]. Since S. aureus was the first human pathogen treated with antibiotics, strains resistant to the antibiotics already emerged a while ago, e.g. resistance against β-lactame antibiotics as penicillin, methicillin (Methicillin Resistant S. Aureus—MRSA) or vancomycin (Vancomycin Resistant S. Aureus—VRSA) [Hiramatsu et al., 1997], [Zetola et al., 2005], [Dantes et al., 2013], [Gardete and Tomasz, 2014].

The genome of a S. aureus strain, which is resistant to vancomycin (Mu50), encodes five putative GH73 family (Glycoside Hydrolase Family 73) members. Four of them, SAV2307, SAV1052, SAV1775, and SAV2644, are widely distributed through the genomes of S. aureus strains. In addition, the genome of S. aureus Mu50 strain encodes SAV0909, which was inserted into the genome through the integration of Bacteriophage phi mu1.The best studied among them is (SAV1052) Major Bifunctional Autolysin (AtlA) [Oshida et al., 1995]. The AtlA deletion mutants form large cell clusters and are biofilm negative [Heilmann et al. 1997, Biswas et al. 2006, Sugai et al. 1995]. The AtlA gene encodes two activities: amidase and glucosaminidase encoded at the N-terminal and C-terminal regions of the sequence, respectively. The amidase activity of AtlA was confirmed and analyzed by structural studies of a homologous enzyme from Staphylococcus epidermis [Zoll et al., 2010] and later S. aureus [Buttner et al, 2014]. However, the glucosaminidases and their role in biofilm formation remained unexplored.

The crystal structures of two GH73 members from Lysteria monocytogenes [Bublits et al., 2009] and of the C-terminal domain of the flagellar protein FlgJ from Sphingomonas sp. [Hasihimoto et al., 2011] and recently endo-N-acetylglucosaminidase from Streptococcus pneumoniae (LytB SP) [Bai et al., 2014] were determined. While the first two proteins exhibit only remote sequence homology to S. aureus, the last one is in part closely related, yet distinct from GH73 family of glucosaminidases from S. aureus. This indicates that members of the GH73 family are sequentially divergent and constitute distinct subfamilies.

General Description of the Invention

It is a general object of the present invention to provide means and methods to find novel antibiotics with activities against a pathogenic, antibiotic resistant bacteria, in particular S. aureus. As a consequence of the experimental results disclosed herein, it was surprisingly discovered that, due to the specific recognition mechanism of NAG-NAM binding by glucosaminidases disclosed herein, potential inhibitors against glucosaminidases can be easier identified than inhibitors against muramidases.

Therefore, the present invention concerns a crystal of autolysin E (AtlE) of Staphylococcus aureus (S. aureus), or a crystallizable fragment of AtlE, and a method for producing said crystal with the help of a special crystallization buffer or a kit containing the special crystallization buffer.

The present invention also concerns a method for screening a binding compound or inhibitor of the N-acetylglucosaminidase activity of AtlE of S. aureus on the basis of said crystals, e.g. by obtaining atomic spatial relationship data followed by in silico screening.

DETAILED DESCRIPTION OF THE INVENTION

According to the present invention it was discovered that autolysin E (AtlE) encoded by the SAV2307 gene is a glucosaminidase with a surprisingly unique active site. Comparison of binding of NAG-NAM or muropeptide (MurP) (NAM-ALA-D-GLU) to AtlE, and polyNAG and NAM saccharides to lysozymes revealed the differences of N-acetylglucosaminidases and murein hydrolases. A comparison of the effects of AtlE and glu-AtlA on S. aureus living cells in a biofilm formation assays indicated that these enzymes are involved in distinct cellular pathways of S. aureus pathogenesis, despite their high sequence homology.

A comparison of the structures of AtlE and lyzozyme complexes explains the difference between the glucosaminidase and muramidase activities. Since the peptidoglycan substrate is the same, each kind of enzyme must approach the substrate from a different side in order to achieve the productive binding.

A specific, conserved region among the GH73 family members of S. aureus indicates that successful antibacterial drugs can be developed to target specific species.

Consequently, the present invention concerns a crystal of AtlE of S. aureus, or a crystallizable fragment of AtlE. In a preferred embodiment said AtlE, or a crystallizable fragment of said AtlE, contains a glutamic acid or an equivalent thereof as the catalytic residue of the enzyme or the fragment thereof. The equivalent of the glutamic acid can be either a functionally active amino acid, e.g. an acidic amino acid, like aspartic acid, or a functionally non-active amino acid, e.g. a hydrophobic amino acid, e.g. alanine, valine, isoleucine, leucine, or phenylalanine. The crystallizable fragment of AtlE can, for example, be a N-terminal truncation of AtlE which particularly still contains the active site of the molecule, where the catalytic residue resides. Consequently, a preferred example of a crystallizable fragment of AtlE lacks the N-terminal transmembrane region, e.g. as shown in FIG. 2 (SEQ ID NO: 2). Alternative fragments of AtlE comprise the amino acid sequence of the glucosaminidase domain of SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO: 6. These domains are highly conserved among the strains of S. aureus.

In addition, the above mentioned crystal can also be a co-crystal of AtlE or its crystallizable fragment, as explained above, and a substrate, ligand and/or a candidate compound. Such substrate, ligand or candidate compound can be a natural or synthetic compound, e.g. NAG-NAM, or any other compound mentioned herein, or a chemical substance from a chemical substance library, in particular from a chemical compound library as stored in a computer.

Said crystal or crystallizable fragment thereof is, in particular, characterized by its space group symmetry and/or its unit cell dimensions. In the present case the space group symmetry can be P2₁P2₁P2₁. The unit cell dimensions can be as follows: a=46.6 Å±1-2 Å, b=69.9 Å±1-2 Å and c=73.3 Å±1-2 Å, in particular a=46.6 Å±1 Å, b=69.9 Å±1 Å and c=73.3 Å±1 Å, with α=90°, β=90° and γ=90°. Specific examples of unit cell dimensions can also be taken from Table 1. The specific X-ray diffraction data can be taken from Table 2.

The present invention additionally concerns a method for producing said crystal or a crystallizable fragment thereof. In a preferred embodiment, the method comprises the steps of

-   -   (a) preparing a solution of said AtlE or a crystallizable         fragment thereof in a crystallization buffer, and     -   (b) crystallizing said AtlE.

The solution, preferably a concentrated solution of AtlE or a crystallizable fragment thereof can be induced to crystallize by several methods including, without limitation, vapor diffusion, liquid diffusion, batch crystallization, dialysis or a combination thereof, preferably vapor diffusion. Generally, in a vapor diffusion method the concentrated solutions of a protein, here AtlE or a crystallizable fragment thereof, become supersaturated and form crystals of the protein at a constant temperature by diffusion of solvent(s), in which the protein is not generally soluble, into the protein solution. Devices for promoting crystallization can include, without limitation, the generally known hanging-drop, sitting-drop, sandwich-drop, dialysis or microtube batch devices. The hanging-drop, sitting-drop, sandwich-drop and some adaptations of the microbatch methods produce crystals by vapor diffusion. For example, the hanging-drop, sitting-drop or sandwich-drop containing the crystallizable composition is equilibrated in a reservoir containing a higher or lower concentration of the precipitant. As the drop approaches equilibrium with the reservoir, the saturation of the protein in the solution leads to the formation of crystals. In the present case the crystallization drop preferably contains equal amounts of the protein solution and of a crystallization buffer. The crystallization buffer in particular contains NaCl and (NH₄)₂SO₄, preferably 2 M NaCl and 2 M (NH₄)₂SO₄. As explained above, the crystallization process can be preferably initiated by a vapor diffusion method. In a particularly preferred embodiment, the crystallization drop contains equal amounts of a protein solution containing AtlE or a crystallizable fragment thereof, e.g. a concentrated protein solution in HEPES buffer, as for example 15 mg/ml protein in 20 mM HEPES and 100 mM NaCl (pH 7.5), and a crystallization buffer, e.g. the buffers as mentioned above. After the crystallization of the protein, the crystals can be cryoprotected by soaking in the crystallization buffer containing a cryoprotectant, e.g. glycerol, in particular 30% glycerol. Generally, the crystallization process is preferably carried out at a constant temperature, e.g. in a range of 10° C.±1° C. to 37° C.±1° C., specifically from 18° C.±1° C. to 25° C.±1° C., more specifically at room temperature, e.g. at 22° C.±1° C.

Consequently, the invention is also directed to crystals obtained by the method of the present invention, and to a kit containing a solution of autolysin AtlE, or a crystallizable fragment thereof as explained above, e.g. in said HEPES buffer or an equivalent buffer, and a crystallization buffer, preferably containing NaCl and (NH₄)₂SO₄, in particular 2 M NaCl and 2 M (NH₄)₂SO₄.

As already explained, said crystal or the characteristic data of said crystal can be used to identify novel, naturally occurring or synthetic, binding compounds, e.g. a novel ligand or a novel inhibitor, of the N-acetylglucosaminidase activity of AtlE and/or the other four enzymes of the GH73 family of S. aureus either by means of a classical screening assay or by means of a computer (in silico screening).

Therefore, the present invention additionally concerns a method for screening a binding compound or inhibitor of the N-acetylglucosaminidase activity of AtlE. Preferably said method comprises the steps of:

-   -   (a) providing a solution of said AtlE or a crystallizable         fragment thereof,     -   (b) contacting at least one candidate compound with the AtlE in         said solution,     -   (c) preparing crystals of said AtlE, and     -   (d) identifying a binding compound of said AtlE.

The solution can be the same solution as explained above. Prior to or after contacting at least one candidate compound with the AtlE or a crystallizable fragment thereof, a crystallization buffer can be added. Preferably the candidate compound, e.g. a naturally occurring or synthetic compound as explained above, is soluble in said HEPES puffer with or without a crystallization buffer, preferably the crystallization buffer as mentioned above. In a particularly preferred embodiment, a solution of AtlE or a crystallizable fragment thereof in said HEPES buffer, or an equivalent buffer, is provided which may already contain the candidate compound. Alternatively, the candidate compound is added to the solution of said AtlE or a crystallizable fragment thereof. The preparation of the crystals can be enhanced by a vapor diffusion method. After having obtained the crystals, the crystals are analyzed with respect to the binding of any candidate compound. For example, in step (d) the binding of the candidate compound to the active site is determined. The active site is preferably characterized by the regions of SEQ ID NO: 1 from methionine 47 (M) to glutamic acid 65 (E), from leucine 136 (L) to glycine 140 (G), from asparagine 159 (N) to glutamic acid 181 (E), from phenylalanine 196 (F) to asparagine 204 (N) and/or from proline 219 (P) to lysine 233 (K), and in particular characterized by the catalytic glutamic acid (E) at position 138, and optionally further by an aspartic acid (D) at position 167, an phenyl alanine (F) at position 224, an aspartic acid (D) at position 227 and/or a tyrosine (Y) at position 201

An inhibitor of AtlE can, for example, be identified by analyzing the binding of a candidate compound to the said active site. Candidate compounds which bind to said active site can, therefore, be identified as novel, naturally occurring or synthetic, ligands or inhibitors of AtlE and the GH73 family members from S. aureus. Examples of such candidate compounds or binding compounds can be antibodies or small molecules with molecules below 600 Da. binding fragments thereof, or fragments of bacterial cell wall components and their derivatives or biopolymers like, but not limited to single chain antibodies or Fv-fragments or ankyrines or DNA fragments.

Consequently, the present invention also provides data to computational methods for using the crystal structure of the AtlE protein and/or of an AtlE-binding compound complex as explained above, e.g. the atomic spatial relationship data, to screen for, identify, design, or optimize a compound binding to AtlE and the GH73 family members from S. aureus. Various computational methods for structure determination and modeling of the protein structure or a protein-complex structure can be used, in particular to evaluate the binding of a binding compound to the active site, as explained above. Such analyses can be carried out in well known crystallographic software applications, such as HKL-3000 software [Minor et al., 2006], the MAIN software [Turk, 2013], the REFMAC software [Murshudov, 1997] and/or the molecular modeling from sequence through lead optimization (e.g. SYBYL® from Certara, L. P., Princeton, N.J., USA). The computational method usually contains the following four steps:

-   -   (a) loading the structure coordinates of the structures to be         compared, here e.g. the structure coordinates of AtlE, or a         crystallizable fragment thereof, as disclosed herein, and the         structure coordinates of a candidate compound e.g. obtained from         a data bank;     -   (b) defining the atom equivalences in these structures;     -   (c) performing a fitting operation on the structures, e.g.         including the crystal structure determination, in particular         including steps such as molecular replacement, model building         and rebuilding, and/or refinement;     -   (d) and analyzing the results, e.g. on the basis of the         structure coordinates of a complex of AtlE, or a crystallizable         fragment thereof, and a candidate compound, as e.g. obtained by         the method disclosed herein;         or alternatively:     -   (a) loading the structure coordinates of the structures to be         compared, here e.g. the structure coordinates of AtlE, or a         crystallizable fragment thereof, as disclosed herein, and the         structure coordinates of a candidate compound e.g. obtained from         a data bank;     -   (b) specifying selected residues of interest, e.g. the         residue(s) of the active site, as disclosed herein;     -   (c) defining the atom equivalences in the selected residues of         interest;     -   (c) performing a fitting operation on the selected residues of         interest;     -   (d) and analyzing the results, e.g. on the basis of the         structure coordinates of a complex of AtlE, or a crystallizable         fragment thereof, and a candidate compound, as e.g. obtained by         the method disclosed herein.

Therefore, the crystal(s) as described herein can also be used for in silico screening of the ability of a candidate compound to bind to said AtlE and/or the other four enzymes of the GH73 family of S. aureus, in particular to bind to the active site of said related autolysins, as explained herein.

Structure coordinates for candidate compounds, other substrates and/or complexes with AtlE or a crystallizable fragment thereof can either be obtained from a data bank, e.g. the RCSB Protein Data Bank (RCSB PDB), Marseille Protein Crystallization Database (MPCD), and any other data bank containing crystal data, e.g. the CRC Handbook of Chemistry and Physics, or from X-ray crystallography as e.g. described herein.

The present invention also encompasses a machine-readable data storage medium comprising a data storage material encoded with machine-readable data, wherein said data comprises the atomic spatial relationship data as detailed above, i.e. at least the space group symmetry and the unit cell dimensions of AtlE or a crystallizable fragment thereof. A machine-readable data storage medium can also comprise structure coordinates of a candidate compound. The computational processing can be performed on a computer as explained above. The computer of the present invention, therefore, comprise a working memory for storing instructions for processing the machine-readable data, a central-processing unit coupled to the working memory and to the machine-readable data storage medium for processing the machine-readable data into the three-dimensional structure. Usually, the computer further comprises a display for displaying the three-dimensional structure as a graphical representation e.g. produced by a software program to display the graphical representation. Such software programs are commercially or freely available.

Consequently, a computer of the present invention comprises executable code for:

-   -   (a) using structure coordinates as disclosed herein;     -   (b) analyzing a binding site of the 3-dimensional model, in         particular the active site as explained herein; and     -   (c) screening in silico a library of candidate compounds; and         optionally     -   (d) controlling a unit for assaying a potential binding compound         identified in step (c) in a protein binding assay or an         enzymatic competition assay using e.g. a synthetic substrate as         disclosed herein.

With the respect to the enzymatic competition assay, the substrate competes with the potential binding compound at the active site of AtlE or a fragment thereof containing the active site, e.g. an AtlE lacking the N-terminal transmembrane region as disclosed herein. In case the substrate is not bound or not cleaved or degraded by AtlE or said fragment, the potential binding compound is identified as an active binding compound, e.g. as a ligand or inhibitor of the N-acetylglucosaminidase activity of AtlE.

Therefore, the hardware components of a computer comprises a machine-readable data storage medium comprising a data storage material encoded with machine-readable data of the present invention, a working memory for storing instructions for processing the machine-readable data of the present invention, a central processing unit (CPU) coupled to the working memory and to the machine-readable data storage medium for processing the machine readable data of the present invention as well as instruction(s) for generating 3-dimensional structure information in particular of the active site as described according to the present invention, and output hardware coupled to the CPU for outputting 3-dimensional structure information and optionally for assaying a potential binding compound identified in a protein binding assay or an enzymatic competition assay as described above. The output hardware usually includes monitor(s), touchscreen(s), printer(s), modem(s), CD-ROM(s) and/or robot(s), i.e. a high-throughput robotic system.

Finally, the structure information or atomic spatial relationship data as disclosed herein can be used e.g. in conjunction with a computer or at least a machine-readable data storage medium for e.g. identifying, designing or even optimizing a binding compound, as described herein, by e.g. performing a fitting operation between a binding compound and the 3-dimensional structure information of AtlE or a fragment thereof containing the active site as described herein. Therefore, the present invention also concerns a method for evaluating the potential of a candidate compound to associate with AtlE and the GH73 family members from S. aureus or said fragment, as described herein, e.g. comprising the steps of

-   -   (a) performing a fitting operation between the candidate         compound and AtlE or a fragment thereof;     -   (b) analyzing the results of said fitting operation to quantify         the association between the candidate compound and AtlE or a         fragment thereof; and     -   (c) illustrating said quantified association preferably in the         form of a 3-dimensional structure or graphical representation         thereof, e.g. on a output hardware such as a monitor or printer.

FIGURES

FIG. 1 concerns the effect of AtlE, glu-AtlA, and the catalytic mutant of AtlE on biofilm formation of S. aureus cells. The figure shows the extent of biofilms formed. The results are shown on the same scale. Each group of results was performed in the same manner by addition various concentrations of the proteins added in the solution as indicated at the left.

FIG. 2 concerns the amino acid sequence of AtlE with the putative transmembrane region. The gray print indicates the part excluded from expression.

FIG. 3 concerns the fold of AtlE with the secondary structure elements. Also the conserved four helices in the core region are shown and the secondary structure elements belonging to the L- and R-lobes.

FIG. 4 concerns the structural similarity of AtlE. AtlE and the related structures of LytB SP (4Q2W), Lmo (3Fl7), FlgJ (3K3T), ACOD (3GXK), and HLYZ (1IWT) are presented in the order from the top to the bottom. The left column shows the chain trace with the secondary structure elements in the same orientation, whereas in the column on the right, the architecture of the folds is presented schematically. The helices are show as cylinders and β strands as arrows.

FIG. 5 concerns the structure based sequence alignment of AtlE, LytB SP, ACOD, LMO, FLGJ, HLYZ of the proteins from FIG. 4. Alignment was performed with Strap [Gille and Frommel, 2001]. The regions at N-termini, which do not exhibit any similarity among the structures, were excluded from this alignment. Hyphens correspond to deletions, whereas dots, small and capital characters correspond to the identical residues, similar and different residues to the sequence on the top, respectively. The catalytic E and the residues, which are important in substrate binding, are marked with an asterisk. 3D images of folds were prepared with Chimera and MAIN [Turk, 2013] and rendered with Raster3D [Merrit, 1997].

FIGS. 6 and 7 concern complexes of AtlE with NAG-NAM and muropeptide. AtlE structure is presented with transparent surface which makes the regions and residues in contact with the ligands visible. Electron densities around the ligands are shown as wired model in FIG. 6 (muropeptide) and FIG. 7 (disaccharide NAG-NAM) contoured at 0.8 and 1.2σ, respectively. The ligands residues and AtlE residues in contact with the ligands are marked and their side chain drawn in stick representation. The main chain is drawn thicker. The binding sites are built from three regions as shown. The figures were prepared with MAIN [Turk, 2013] and rendered with Raster3D [Merrit, 1997].

FIG. 8 concerns a comparison of similar ligands superimposed to AtlE structure. AtlE is shown as transparent white surface with the catalytic E138 side chain labeled. The crystal structures of muropeptide and NAG-NAM disaccharide determined in the complex with AtlE are shown as stick models. They are marked as “MurP” and “DISACCHARIDE”. The muropeptide ligand bound to T4 lysozyme (148 L) is marked with T4 MurP. The di- and tri-saccharide structures determined in the complex with ACOD (3GXR) are marked with ACOD NAG. The figure was prepared with MAIN [Turk, 2013] and rendered with Raster3D [Merrit, 1997].

FIG. 9 concerns a comparison of parts of the amino acid sequences corresponding to glucosaminidase domains of S. aureus GH73 family members. Multiple sequence alignments of sequences with AtlE are shown on the top. Hyphens correspond to deletions, whereas dots, small and capital characters correspond to the identical residues, similar and different residues to the sequence on the top, respectively. The regions defining the active site are marked with numbers 0, 1, 2, 3, 4. The alignment was made with Clustalw.

FIG. 10 concerns the surface representation of AtlE structure with mapped identity of residues. Amino acids are shadowed according to their identity corresponding to the counts of dots in the FIG. 9). Dark shadows reflect 100 % identity, whereas lighter shadows indicate lower levels of similarity. White indicates no identity. The view into the active site is shown. The figure was prepared with MAIN [Turk, 2013] and rendered with Raster3D [Merrit, 1997].

FIGS. 11 and 12 show the structural differences between glucosaminidases and muramidases (lysozymes) in binding of glycan cell wall components. Images of 3D models were prepared with MAIN [Turk, 2013] and rendered with Raster3D [Merrit, 1997].

FIGS. 11a ) and 12 a) represent a schematic presentation of approach of glucosaminidases and muramidases to the poly NAG-NAM saccharide, where the lactyl moieties are oriented towards L- and R-lobe, which correspond to glucosaminidase and muramidase binding, respectively.

FIGS. 11b ) and 12 b) show models of hexasaccharide (NAG-NAM)₃ bound into the AtlE and ACOD active sites. They are shown in ball and stick presentation against the surface of the targeted enzyme. Nitrogen and oxygen atoms are in bold face as in the hexasaccharide model shown in FIG. 13.

FIG. 13 shows a model of NAG-NAM-NAG-NAM tetra-saccharide with cleavage sites assigned. The ring and glycosidic bonds are shown with thick sticks and atom balls of corresponding radii, whereas the lactyl and amide groups are shown as thin sticks and correspondingly small atomic radii. The cleavage sites by muramidases and glucosaminidases are marked.

FIG. 14 is a schematic representation of the tetrasaccharide substrate with the expected digestion products.

FIGS. 15 and 16 show the analysis of AtlE and gluAtlA digestion products of (NAM-NAG)2red substrate. FIG. 15 is a mass spectrometer analysis of the digestion products of AtlE, and FIG. 16 of the digestion products of gluAtlA. The expected molecular peaks are annotated. The same amount substrate/digestion products were analyzed in both cases.

FIG. 17 shows the synthesis of the disaccharide NAG-NAM with the following reagents and conditions: a) Et₃SiH, I₂, 0° C., 2 h; b) AgTf, rt, 18 h; c) NaOMe, MeOH, rt, 1 h; hydrazine hydrate, EtOH, 80° C., 2 h; pyridine, acetic anhydride, rt, 18 h; d) 0.5 M KOH, dioxane, rt, 48 h; H₂, Pd/C, EtOH:HOAc:water; rt, 18 h.

FIG. 18 shows the effect of mutations of residues involved biochemical analysis of cell wall degradation. Fold of AtlE structure is shown in light. The side chains of mutated residues are shown in ball and stick presentation. Oxygen and carbon atoms are shown in dark. The residues are marked. The radii of atom balls of the mutated residues correspond to the level of reduced activity of the mutants. So, the 100% drop of activity corresponds to the 100% of VdW radii of side chain atoms, whereas the mutated resides which did not effect activity are shown as sticks only. E138 and Y201 mutants resulted in zero activity, the D167A and D227A resulted in halved activity, whereas E145A mutant exhibited the activity of native enzyme.

ABBREVIATIONS

AtlE: autolysin E; DCM: dichloromethane; ESI-MS: electrospray ionization mass spectrometry; EtOAc: ethyl acetate; EtOH: ethanol; GH73 domain: C-terminal domain of LytB SP (Streptococcus pneumoniae); HOAc: acetic acid; iPrOH: isopropanol; MeOH: methanol; MurP: muropeptide (NAM-ALA-D-GLU); NAG: N-acetylglucosamine; NAM: N-acetylmuramic acid; Phth: phthaloyl; SeMet: seleno-methionine; rt: room temperature; VdW: Van der Waals;

EXAMPLES

A) Materials and Methods

1. Expression of Recombinant Proteins

AtlE is 259 amino acid long protein encoded by the SAV2307 gene loci in the genome of S. aureus strain Mu50. The truncated sequences of glucosaminidase domains of AtlE, lacking the first 34 residues and glu AtlA from S1012 onwards, include additional four amino acids (SAAA, i.e. Ser-Ala-Ala-Ala) that belong to the recognition site of TEV protease. The nucleotide sequences were amplified from the genomic DNA of S. aureus Mu50 using KOD Hot Start Polymerase and cloned into pMCSG 7 plasmid in the frame with N-terminal His-Tag as described [Eschenfeldt et al., 2009]. The mutants were prepared by the overlap extension method [Ho et al., 1989]. The proteins were expressed in BL21(DE3) E. coli expression strain grown in YZM5052 auto induction medium. To facilitate the expression of proteins in soluble form, the cells were initially grown at 37° C. When optical density (OD) measured at 600 nm reached the value of 1, the cells were transferred to 25° C. After 16 hours the cells were pelleted by centrifugation (15 minutes at 7000× g), re-suspended in the buffer A (0.03 mM Tris, 0.4 M NaCl, pH 7.5) supplemented with 1 mg/ml of lysozyme, and frozen and disrupted by the freezing thawing cycles and sonication. The proteins were purified from the cell lysate on AKTAxpress FPLC system (GE Healthcare) using two-step purification protocol. The first purification step was Ni²⁺-affinity chromatography on HiTrap IMAC FF column (GE Healthcare) equilibrated in buffer A with 10 mM imidazole. The bound proteins were eluted with the buffer A containing 300 mM imidazole and applied to the HiPrep 26/60 Sephacryl S-200 size exclusion column (GE Healthcare) equilibrated in the buffer A. The fractions containing the pure protein were collected, concentrated, desalted against 20 mM HEPES, 100 mM NaCl, pH 7.5, and stored at −20° C.

2. Biochemical Analysis of AtlE and AtlA Activities

AtlE and glu AtlA were tested against S. aureus cell wall and against two synthetic substrates (NAM-NAG)_(2red) tetrasaccharide (Figure S1) and (NAG)_(6red). The degradation products were analyzed by mass spectroscopy. A single cleavage between the central NAG-NAM residues should indicate the classic N-acetyl-glucosaminidase activity by generating the NAM-NAG and NAM-NAG_(red) disaccharides, whereas NAM_(red) and NAG-NAM-NAG_(red) would indicate the muraminidase activity. In accordance with expectations only NAM-NAG_(red) with Mw of 499.21 Da was found. Besides, the glucosaminidase activity of the putative glucosaminidase domain from AtlA (glu AtlA) was tested and exhibited the same specificity profile (FIG. 1c ). Surprisingly, the (NAG)6 was not degraded at all.

3. Isolation of S. aureus Peptidoglycan

S. aureus cells were grown overnight in Brain Hearth Infusion Broth (BHI) (37° C., 250 rpm) pelleted by centrifugation (15 minutes at 6000× g), washed three times with the 30 mM Tris, 0.4 M NaCl buffer and then re-suspended in the same buffer. After heat inactivation, the insoluble pellet was re-suspended in 4% SDS and incubated 30 minutes at 80° C. After 30 minutes of centrifugation at 40,000× g, the pellet was re-suspended and washed six times in water. Proteins that remained associated with the peptidoglycan were digested overnight by incubation with trypsin (1 mg/ml) in 30 mM Tris, 0.4 M NaCl, 10 mM MgCl2, pH 7.5 buffer. After pelleting (30 minutes at 40000× g) peptidoglycan was treated overnight with 5 ml 40% aqueous hydrogen fluoride to remove teichoic acid, pelleted again (30 minutes at 40000× g) and washed extensively with water. Saturated suspension of the pure peptidoglycan solution in water was stored at −20° C.

4. Biochemical Peptidoglycan Degradation and Labeling with Remazol Brilliant Blue

For the colorimetric assays, peptidoglycan was labeled with Remazol Brilliant Blue as described [Odintsov et al., 2004]. In short, 50 ul of water suspension of Remazol Brilliant Blue labeled peptidoglycan was mixed with 10 ul of 1 M buffer solutions at pH of 4.5, 5. and 5.5, 25 ml of 4 M NaCl, different amounts of enzymes and filled with water to the final volume 100 ml. The final concentration of buffer and NaCl in the reaction mixture was therefore 100 mM. During the reaction the sample was shaken at 30° C. and in time intervals 10 ml aliquots were taken from reaction mixture, centrifuged for 3 minutes at 15000× g and the absorbance at 595 nm was measured on NanoVue spectrophotometer (GE Healthcare).

Depending on the desired pH the following stock buffer solutions were used: 1 M NaOAc pH 4.5, 5 and 5.5, 1 M potassium phosphate pH 6 and 6.5 and 1 M Tris, pH 7.0, 7.5, 8 and 8.5.

5. Biofilm Formation Assay

The effect of recombinant autolysins on the S. aureus biofilm formation was performed as described [Merritt et al. 2011]. In brief, saturated culture of S. aureus grown overnight in BHI medium was diluted 1:100 in buffered BHI medium (supplemented with 0.1 M phosphate buffer pH 6.0 containing 0, 0.5, 1, 2 and 5 μM concentration of individual enzyme. 100 μl of each diluted culture was pipetted in 96-well plate (TPP). The plates were covered and incubated in a humidified incubator at 30° C. After 48 hours, the media was removed and the plates were three times washed with water. After the last washing step all remaining liquid was removed and 125 μl of 0.1% crystal violet was added to each well to dye the attached cells. After 10 minutes of incubation at the room temperature, the solution of crystal violet was removed and plates were washed several times with water until all unbound dye was removed. After the plates were completely dried at room temperature, the dyed biofilms were dissolved in 200 μl of 30% acetic acid. After 20 minutes of incubation at room temperature, the plates were briefly mixed and 100 μl of the crystal violet/acetic acid solution was transferred to a fresh 96 well micro-titer plate and the optical density of the samples was measured ad 595 nm. All experiments were performed in triplicates with AtlE, AtlA A138E mutant, and glu-AtlA domain assayed at pH 6.

6. Seleno-Methionine Derivative Expression

The structure of AtlE was phased with the help of seleno-methionine residues because at the time of structure determination no close homologues of AtlE were found in the protein data bank of RCSB (PDB database). SeMet minimal medium (SeMetMM) [Guerrero et al (2001)] was prepared by dissolving NH₄Cl (1 g), KH₂PO₄ (3 g) and Na₂HPO₄·7H₂O (6 g) in 1 l of deionised water and autoclaved. To this, 100 ml of a filter-sterilized solution containing 20% (w/v) glucose, 0.3% (w/v) (MgSO₄), 10 mg Fe₂(SO₄)₃ and 10 mg thiamine were added and the pH adjusted to 7.4. Finally, L-SeMet was added to the medium to a final concentration of 50 μg/ml.

A subculture of the E. coli BL21(DE3) pMCSG7-AtlE transformants was grown overnight in 20 ml of LB medium supplemented with ampicillin (100 μg/ml) at 37° C. with shaking at 250 rpm. The next day, this cell suspension was used as the inoculum for 1 l of the same medium and OD600 was measured until it reached the value of 1. Cell culture was then centrifuged for 15 min at 4000 rpm and the pellet was re-suspended in 1 l of SeMet minimal medium followed by adding IPTG to a final concentration of 1 mM and incubation at 18° C. and 250 rpm for additional 20 hours.

7. NAG-NAM Disaccharide Synthesis

The NAG-NAM disaccharide 2-acetamido-4-O-(2-acetamido-2-deoxy-β-d-glucopyranosyl)-3-O-[(R)-1-carboxyethyl]-2-deoxy-α-d-glucopyranose (NAG-NAM) was prepared, with some revisions, according the protocol introduced by Kantoci and Keglević (1987) and papers cited therein (Figure S2). Selective opening of the 4,6-benzylidene ring of benzyl 2-acetamido-4,6-O-benzylidene-3-O-[(R)-1-(methoxy-carbonyl)ethyl]-2-deoxy-α-d-glucopyranoside (1) to give benzyl 2-acetamido-6-O-benzyl-3-O-[(R)-1-(methoxycarbonyl)ethyl]-2-deoxy-α-d-glucopyranoside (2) was performed with iodine and triethylsilane instead of sodium cyanoborohydride as was previously described (Keglević et al. 1985). Glycosidic bond formation between activated glucosamine 3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-β-d-glucopyranosyl chloride (3) and selectively protected muramic acid 2 in presence of silver triflate in extremely dry conditions gave 2-acetamido-4-O-(3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-β-d-glucopyranosyl)-6-O-benzyl-2-deoxy-3-O-[(R)-1-methoxycarbonyl)-ethyl]-α-d-glucopyranoside (4). Removal of the phthalimido group from compound 4 with hydrazine followed by acetylation gave benzyl 2-acetamido-4-O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-β-d-glucopyranosyl)-6-O-benzyl-2-deoxy-3-O-[(R)-1-(methoxycarbonyl)ethyl]-α-d-glucopyranoside (5). Saponification of acetyl and methyl groups, and removal of benzyl groups with catalytic hydrogenation gave NAG-NAM (Kantoci and Keglević, 1987; Keglević et al., 1985).

Synthesis of Benzyl 2-acetamido-6-O-benzyl-3-O-[(R)-1-(methoxycarbonyl)ethyl]-2-deoxy-α-d-glucopyranoside (2)

Compound 1 (630 mg; 1.3 mmol) was dissolved in dry DCM (10 mL), iodine (370 mg) and Et₃SiH (3.7 mL) were added. Reaction was stirred in ice-bath and after 30 min and 1 h additional portions of iodine (37 mg) and Et₃SiH (370 μL) were added. Reaction was finished after 2 h, diluted with DCM (40 mL) and washed with (a) NaHCO₃ (20 mL) and (b) water (20 mL). Organic layers were dried with Na₂SO₄ evaporated and chromatographed on silica gel column in solvent systems DCM:acetone 3:2 and DCM:MeOH 9:1. Crystallization from acetone:diisopropyl ether gave compound 2 (330 mg; 52%). ESI-MS: C26H33NO8 488.4 [M+H]⁺ calc. 488.5; Rf=0.65 (DCM:MeOH 9:1).

Synthesis of Benzyl 2-acetamido-4-O-(3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-β-d-glucopyranosyl)-6-O-benzyl-2-deoxy 3-O-[(R)-1-(methoxycarbonyl)ethyl]-α-d-gluco-pyranoside (4)

The glucosyl chloride 3 (280 mg; 0.62 mmol) and protected muramic acid 2 (100 mg; 0.21 mmol) with silver triflate (AgTf; 210 mg; 0.82 mmol) as catalyst were subjected to the Anderson's apparatus for glycosidic coupling (Nashed and Anderson, 1982), molecular sieves and dry DCM (2 mL) were added and reaction was stirred under nitrogen at room temperature overnight. Thereafter, chloroform was added to the formed suspension and centrifuged. The residue was washed two times with chloroform. Collected chloroform's supernatants were washed with saturated aqueous solution of NaHCO₃, water, and dried over Na₂SO₄. Solvent was evaporated and product purified by flash silica gel column chromatography in solvent systems diethyl ether:petroleum ether:iPrOH 8:4:1 and DCM:MeOH 9:1. After the second column compound 4 (62 mg; 33%) was obtained.

ESI-MS: C46H53N2O17 905.4 [M+H]⁺ calc. 905.3; C46H52N2NaO17 927.4 [M+Na]⁺ calc. 927.3; Rf=0.54 (diethyl ether:petroleum ether:isopropanol 8:4:1).

Benzyl 2-acetamido-4-O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-β-d-glucopirano-syl)-6-O-benzyl-2-deoxy-3-O-[(R)-1-(methoxycarbonyl)ethyl]-α-d-glucopyranoside(5)

Disaccharide 4 (45 mg; 0.0498 mmol) was dissolved in dry MeOH (1.376 mL) with addition of 0.1 M NaOMe/MeOH (145 μL). Reaction was stirred at room temperature for 1 h after which additional portion of 0.1 M NaOMe/MeOH (145 μL) was added and stirring was continued for 15 min. The reaction solution was neutralized with Amberlite IR-120 (H⁺), filtrated and evaporated. The residue was dissolved in 96% EtOH (2.25 mL) and hydrazine hydrate (16.88 μL). The reaction was stirred for 2 h under reflux (80° C.). Reaction mixture was evaporated with toluene addition. Residue was dissolved in pyridine:acetic anhydride 1:1 (1.2 mL) and stirred overnight. After that solvent was evaporated with toluene addition and the residue was purified by flash silica gel column chromatography in EtOAc:iPrOH:petroleum ether 2:3:1 to give compound 5 (27 mg; 67%).

ESI-MS: C40H52N2NaO16 839.3 [M+Na]⁺ calc. 839.3; Rf=0.50 (EtOAc: iPrOH: petroleum ether 2:3:1).

2-Acetamido-4-O-(2-acetamido-2-deoxy-β-d-glucopyranosyl)-3-O-[(R)-1-carboxy-ethyl]-2-deoxy-α-d-glucopyranose (NAG-NAM)

Compound 5 (35 mg; 0.043 mmol) was dissolved in dioxane (1.75 mL) and 0.5 M KOH (0.875 mL) was added to adjust pH 12. Reaction was stirred at room temperature 48 h, and then neutralized with addition of Amberlite IR-120 (H⁺), filtered and evaporated. Residue was dissolved in EtOH:HOAc:water 6:1.5:1.5 (5.25 mL) and Pd/C (10%; 46 mg) was added. The reaction was hydrogenated at room temperature overnight. After that the reaction was filtered over small column of Celite to remove catalyst, and filtrate was evaporated. The residue was crystallized from MeOH:ether 1:10 to give NAG-NAM (15 mg; 70%).

ESI-MS: C19H32N2NaO13 519.2 [M+Na]⁺ calc. 519.2; Rf=0.55 (n-butanol:HOAc:EtOAc:water 1:1:1:1).

8. Protein Crystallization and Structure Determination

The crystals of AtlE E (concentrated to 15 mg/ml in 20 mM HEPES, 100 mM NaCl, pH 7.5) were grown in 2 M NaCl, 2 M (NH4)₂SO₄ using vapor diffusion method. The crystallization drop consisted of 1 μl of the protein solution and 1 μl of crystallization buffer. The crystals were cryoprotected by soaking in the crystallization buffer containing 30% Glycerol. The native and seleno-methionine (SeMet) derivative crystal diffraction data were collected at Elettra synchrotron at XRD beamline.

The native structure was solved with the help of SeMet derivative with data collected at the remote wavelength exploiting the anomalous signal from seven SeMet residues using HKL-3000 software [Minor et al., 2006]. The native structure was rebuild, refined, and solvent inserted using MAIN [Turk, 2013] and REFMAC [Murshudov, 1997] and deposited to PDB (4PIA).

NAG-NAM disaccharide was synthesized as described above, whereas NAM-ALA-D-GLU (MurP) was purchased. NAG-NAM and MurP complexes were obtained with soaking of native crystals with 10 mM solution of the ligands. Data from crystals of MurP in complex with the native protein and NAG-NAM in complex with E138A mutant were collected at Bessy synchrotrone (Beam line 14.1), whereas the diffraction data for the NAG-NAM in complex with the native enzyme were collected at home X-ray source (Bruker proteum). The diffraction data were integrated with

HKL-2000 [Otwinowski and Minor, 1997]. Structures were build with MAIN using topology library and geometric restraints provided by PURY [Andrejasic et al., 2008] and finally refined with REFMAC for deposition. The geometry of binding of disaccharide was in the two crystal structures equivalent, therefore only the complex with the native sequence is shown in the figures, however, all four crystal structures were deposited in PDB. Data and refinement statistics are provided in Table 1.

9. Identification of Catalytic Residues

The structural similarity of the GH 73 enzymes presented (FIG. 3b ) exposed the E138 (glutamic acid at position 138 of SEQ ID NO: 1) as the catalytic residue in the AtlE structure. Its mutation to alanine indeed abolished its activity in the assay with S. aureus purified peptidoglycan.

Inspection of superimposed structures from FIG. 3 suggested additional candidates for the second carboxylic group involved in the hydrolysis of glycosidic bonds: E145, D167, and D227 due to their position in respect to the similarly positioned acidic residues in Lmo, ACOD, and LytB SP structures. On the R-side E145 is positioned closely to the E129 of the superimposed Lmo structure, D167 is positioned similarly as the D90 and D101 of ACOD, and D227 is equivalent to D262 of LytB SP. The first two residues lie above the catalytic E138 on top of two different features of the L-lobe. The closest distances between the oxygen atoms of carboxylic groups of the catalytic E138 and the two candidates for the secondary catalytic residue D167 and E145 are 11 and 14 Å, respectively. The carboxylic group oxygen of the R-lobe candidate D227 is positioned is 8.4 Å away from the closest oxygen E138. The large distance unlikely makes them the second catalytic residue. Nevertheless, mutants were produced and tested for their activity against the cell wall substrate as described above. The activity of the AtlE D167A and D227A mutants were halved in comparison with the native enzyme, whereas the E145A mutant exhibited activity equivalent to the native enzyme. Hence, D167 and D227 are important residues for hydrolysis of the cell wall, however, the activity of the enzyme was not reduced to the level which would allow us to conclude that either of them is the second catalytic residue. More likely, they are contributing to substrate binding, as for example the Y201, which mutation to alanine abolished the enzymatic activity. From this analysis we concluded that the lack of the second catalytic residue in AtlE suggests a reaction mechanism which is different from the reaction mechanism of lysozyme. Instead of the second carboxylic group, AtlE likely uses of an external nucleophile, likely a water molecule.

10. Molecular Modeling of NAG-NAM Substrates

The hexa N-acetylglucosamine (NAG) was built first. The (NAG)₆ model was build by filling the gap between the (NAG)₂ and (NAG)₃ parts of the ACOD structure [Hellman et al, 2009] (3GXR) with the missing NAG residue. The resulting hexasaccharide was energetically minimized by constraining the matching NAG residues to the experimental structure using software MAIN [Turk, 2013]. In order to build a model corresponding to muramidase activity of lysozymes, residues -3, -1, and 2 were mutated to N-acetylmuramic acid (NAM). Thereby the lactyl group was added to the NAG residue. The resulting hexasaccharide was energetically minimized again.

In order to build the substrate model corresponding to the N-acetylglucosaminidase activity, the structures of AtlE and ACOD were superimposed by software FatCat [Ye et al., 2003]. By use of the superimposition parameters, the (NAG)₆ model was transferred from the ACOD environment to the AtlE structure. Then, the −2, +1, and +3 residues were mutated to NAM, the model was shifted slightly to the right to match the NAG-NAM position in the complex with AtlE, and then energetically minimized by constraining the positions of atoms in −3, −2 residues to the position observed in the crystal structure.

B) Results

1. Characterization of AtlE Activity

Lysozymes (muraminidases) are among the most studied enzymes. They exhibit a broad specificity profile. They cleave poly NAG and (NAG-NAM) substrates, much less it is however known about glucosaminidases. To provide insight into the biochemical activity of AtlE and AtlA the corresponding parts of the sequence were expressed and tested against the S. aureus cell wall and two synthetic substrates (NAM-NAG)_(2red) tetrasaccharide (FIG. 14) and (NAG)_(2red). Both proteins were active against the cell wall substrate and both cleaved only the NAG-β(1,4)-NAM glycosidic bond, and are N-acetylglucosaminidases only, whereas (NAG)₆ was not cleaved at all.

Having confirmed the enzymatic activity of the two proteins, indications about their involvement in the biofilm formation were obtained. AtlE and gluAtlA were added to the solution of S. aureus culture in a biofilm formation assay. Addition of AtlE to the solution increased the formation of biofilms in a concentration dependent manner (FIG. 1). In contrast, addition of gluAtlA exhibited no effect on biofilm formation regardless of the enzyme concentration. To find out whether the induced biofilm formation is a consequence of the catalytic activity of the enzyme, the test with the catalytic mutant E138A of AtlE was also performed. It was demonstrated that the catalytic activity of AtlE in the media is indeed responsible for facilitating the biofilm formation under the applied conditions. Hence, this experiment revealed that different glucosaminidases behave differently and indicated that the presence of AtlE may be important for S. aureus biofilm formation process.

2. Structural Analysis

To gain insight into the 3-dimensional structure of AtlE, the crystal structure of AtlE was determined. The sequence analysis of the full length protein suggested that it consists of a short N-terminal cytoplasmic tail followed by a transmembrane helix (lle9 to Val27) and the outer domain (FIG. 2; SEQ ID NO: 1). The truncated sequence lacking the first 34 residues was expressed and the protein crystallized. The refined model of the naked AtlE structure consists of 228 amino acid, 352 molecules of water and 10 chloride ions.

3. Overall Structure Description

AtlE adopts a heart like globular fold composed of left (L-) and right (R-) domains (FIG. 3). To address structural parts easier, the structure, the core and lobe regions were divided. The compact α-helical structural core is the lower part of both domains, whereas the R- and L-lobes containing short α-helical and β-strand regions reside on the top of L- and R-domain, correspondingly. Between the lobes, there is a long groove that runs across the entire central part of the molecule (FIG. 4). The secondary structure elements in the figures are numbered in the order they follow in the amino acid sequence. The polypeptide chain first builds the R-lobe, then enters the L-domain, in which the L-lobe is inserted between the helices α7 and α12, and then returns into R-domain and builds the R-core region. The core of the structure consists of 6 helices of different length: α5, α6, α7 and α12 from the L-domain and α13 and α14 from to the R-domain, whereas each lobe contains two short α-helices and two β-hairpins. In the L-lobe, the β-hairpin precedes the two α-helices, whereas in the R-lobe the β-hairpin is positioned between them (α1 and α4). Both domains are connected through two regions: an extended loop connecting the α4 and α5 helices and a short linker region connecting the α12 and α13 helices.

4. Similarity to Other Structures

Using the determined structure of AtlE DALI server identified several similar structures. The closest structural homologues were Pneumococcal peptidoglycan hydrolase LytB (LytB SP) (4Q2W, Z-score=16.9) [Bai et al., 2014], autolysin Lmo1076 (Lmo) (3Fl7, Z-score=8.0) [Bublits et al., 2009] and FlgJ (2ZYC, Z-score=7.3) [Hashimoto et al., 2009]. They all belong to the GH73 family of glycosyl hydrolases. The list continued with the G-type lysozyme from Atlantic cod (ACOD) (3GXR) [Hellman et al., 2009] which belongs to GH23 family (Z-score=6.7). Due to the similarity in architecture, even though not spotted by the DALI server, also the human lysozyme structure (HLYZ; 1IWT) was included as a representative of the goose-type lysozymes [Joti et al., 2002]. The structural and sequence alignment of the six enzymes shown in FIG. 4 reflects their structural similarity, but also points out their diversity.

Superimposition of the structures revealed that all six helices comprising the core of the AtlE fold are similar to the C-terminal domain of LytB SP, named “GH73 domain”, whereas only the four helices α6, α7, α12, and α14 shown in dark (FIGS. 3 and 4), which form the central core of AtlE structure, have counterparts in Lmo, FlgJ, HLYZ, and ACOD. (FIG. 4). Conservation of these four helices is typical for the proteins that adopt the lysozyme-like fold. A slight exception is the HLYZ structure in which the C-terminal helix is broken in two parts (α6) and extended. Another exception is also the α3 helix from the ACOD structure which is curved, extended, and wrapped along the inter domain interface from where the chain folds back and around the C-terminal helix and contacts the L-domain from below.

The L- and R-cores are in all compared structures build from a-helical elements (FIG. 4). The L-cores are built similarly, whereas the R-cores differ in the size and structure. The three helices from the L-core of AtlE are present in all compared structures, whereas the AtlE α5 helix is present in the AtlE and LytB SP structures only. In the R-core, the C-terminal helix is present in all structures except HLYZ. In AtlE, and ACOD/GLYZ structures the R-core is built from the N- and C-terminal parts of the chain, whereas in the HLYZ, Lmo, and FlgJ structures the R-core is folded entirely from the C-terminal part of the chain. In the LytB SP structure, the R-core is absent from the GH73 domain apart from the conserved helix, which is positioned similarly as in the Lmo and FlgJ structures.

The R-lobe is unique to AtlE structure and absent in all others. It is build from the N-terminal terminal parts of the sequence. Also in the LytB SP structure, the GH73 domain does not have an R-lobe. Its space is, however, occupied by the N-terminal domain WW.

The L-lobes are present in all listed structures. They are built from the elements of β-structures, yet they differ in the folding pattern and their positioning. Only the HLYZ structure contains a three-strand β-sheet, whereas in the Lmo and FlgJ structures there are long β-hairpins which extend into the upper part of the structure above the inter domain interface. Additional β-hairpins are found in the AtlE and FlgJ structures. They, however, extend towards the left and in the direction away from the inter domain interface. In contrast, the LytB SP has there a small number of residues which structure is partially disordered.

Structural sequence alignment of the six enzymes shown in FIG. 5 performed with STRAP [Gille and Frommel, 2001] shows that the GH73 domain from LytB SP is a close homologue of AtlE. Being insufficient for degradation of cell wall by itself, this comparison provides explanation for the need of the WV domain and the linking SH3b domain [Bai et al., 2014]. This figure also shows that all these enzymes share only a single residue conserved in all sequences. In the AtlE sequence it is E138, the catalytic residue of the lysozyme-like enzymes as confirmed by the abolished activity of the E138A mutant, whereas an additional catalytic residue was not identified. E138 is positioned at the C-terminus of the central helix, α7 in the AtlE structure. This helix lies in the L-domain with the E138 residue positioned at the bottom of the cleft formed between the two domain interface.

To summarize, the AtlE structure is a lysozyme-like enzyme, yet it differs from the currently known ones suggesting that the S. aureus GH 73 family members have unique properties which can be exploited as potential drug targets.

5. Structures of Disaccharide and Muropeptide in Complex with AtlE

To gain insight into the binding of substrate experimentally, the crystal structures of AtlE in complex with disaccharide NAG-NAM and muropeptide NAM-ALA-D-GLU (MurP) were determined.

In FIGS. 6 and 7, three regions, which form the interactive surface for disaccharide and muropeptide binding, and the catalytic residue are shown:

-   -   the α7 on which C-terminus the catalytic E138 resides;     -   the left flank region with G164 involved in binding of NAM         residue and I163 forming a hydrophobic surface for the         muropeptide alanine;     -   the L-lobe region at the back with the aromatic residues F196         and Y201, the later is involved in binding of NAM residue;     -   the L-lobe loop region with Q223 and Y224 residues involved in         binding of N-acetyl moieties of NAM and NAG residue (Y224 is         conserved in all structures, but HLYZ which has W).

Besides the three regions shown in FIG. 8 and indicated in FIG. 9 as regions 2, 3, 4, the substrate binding surface is additionally build from the region on the left with the chain extending from the catalytic E138 which starts the L-lobe and from the region on the right where the K57-V64 loop extends the R-lobe surface. These two regions are indicated as 0 and 1 in the FIG. 9.

In the case of the NAG-NAM complex, only a single molecule of disaccharide was bound to the AtlE active site (FIG. 6). As the closest atom to the catalytic residue E138, the O1 atom from NAM residue is positioned 6.7 Å away from the carboxylic group OE2 atom. The disaccharide is positioned above the Q221-S226 loop shown in green. It is pined to the surface at the bottom of the cleft with four hydrogen bonds, three formed by NAM residue and one by NAG. The N-acetyl group of NAM is pinned to the surface of AtlE by the hydrogen bonds with the main chain atoms G164 NH group and Y224 carbonyl. The oxygen atom of the lactyl moiety of the NAM residue forms hydrogen bond with OH group of Y201 side chain. The N-acetyl group of NAG residue forms hydrogen bond with main chain NH group of Q223. Numerous solvent molecules, two chloride ions and a sulfate ion are positioned in the region around the disaccharide.

In the MupP, the NAM and alanine residues are unambiguously resolved by the electron density map, whereas the positioning of the atoms of D-glutamic acid residue is less defined as indicated by the electron density map (FIG. 7). The NAM moiety of MurP binds to AtlE structure equivalently to that observed in the NAG-NAM AtlE complex (FIG. 6). The alanine hydrophobic side chain is positioned within the hydrophobic environment formed by the side chains of 1163, G164, and F196 whereas the D-GLU residue is disordered and points into the solvent.

6. Substrate Binding Site

To analyze the binding of NAG-NAM disaccharide and the muropeptide in the light of other related complexes, both AtlE complexes were superimposed with the crystal structures of the NAG trisaccharides bound to goose-type lysozyme from Atlantic cod (ACOD) [Hellman et al., 2009], (154 L), with the NAG-NAM-peptide in the complex with T4 lysozyme [Weaver and Matthews, 1987], (148 L), with NAG trisaccharide bound to the goose lysozyme structure of the complex [Weaver et al., 1995] (154 L), and NAM-NAG-NAM trisaccharide in complex with chicken lysozyme [Kelly et al., 1979] (9LYZ). The five resolved NAG carbohydrate rings from the ACOD structure fit into the active site of AtlE. A similar position is also occupied by the NAG trisaccharide in the complexes with the goose lysozyme structure (GLYZ) and chicken trisaccharide (9LYZ). Taken together, these structures indicate the positions of sub-site binding from −3 to +3 using the nomenclature proposed by Davies [Davies, 1997] or the B to G nomenclature as applied in the ACOD structural paper [Hellmann et al., 2009]. According to Davis nomenclature, the observed NAM residues in the AtlE complexes (FIG. 8) bind into the −2 sugar binding subsite and NAG into the −3 subsite.

Hence, the substrate binding site runs across the central core of the AtlE molecule and is limited on the left and right side by the structural elements of L- and R-lobe.

7. Similarity of S. aureus GH73 Family

As the analysis of available crystal structures of glucosaminidases indicated that this group of enzymes differs too much to enable their inhibition with a single compound, the target potential of S. aureus GH73 members was explored. To gain insight into the similarity and diversity of the active sites of the S. aureus GH 73 family members a sequence alignment of the glucosaminidase domains were made (FIG. 9). To visualize distribution of sequential identity, the sum of identical residues at the surface of AtlE were mapped using (FIG. 10), where the dark areas correspond to the position of the conserved residues in all five sequences, whereas the white areas to none. Since the majority of the surface is white, this mapping indicates that also among the S. aureus GH 73 members are substantial differences in their structure. However, the sequentially most conserved region lies in the −3, −2, −1 and +1 substrate binding sub-sites. On the right, G162 is positioned next to catalytic E138. Together with Y224 and A225 residues, they build the surface of −1 and −2 sub-sites. A165 builds the surface above, on the left, S144 is positioned at +2 sub-site below. Interestingly, the Y201 residue involved in the hydrogen bond with the lactyl group of the NAM residue at −2 sub-site cannot be found in SAV1775 and SAV2644 sequences, where there are F and I respectively. Assuming that these two enzymes are glucosaminidases, they likely contain a different anchor for the lactyl moiety. In addition the Y201A mutant of AtlE is completely inactive. These together indicates that potential inhibitors of glucosaminidases from GH73 family of S. aureus should target the indicated conserved area, its immediate surroundings or parts of it that spanns the binding sites from −3 to +2 which spatial relationships of the whole group of S. aureus enzymes from the GH73 famili were revealed by the AtlE crystal structure described herein

C) Analysis of the Results

First of all, the structural basis of substrate recognition of glucosaminidases and muramidases were explored. As explained above, the complexes of hexasaccharides with alternating sequence of NAG NAM residues were modeled into the structure of the active site clefts of AtlE and ACOD as the representative enzymes for the N-acetylglucosaminidase and muramidase activities, respectively. FIGS. 11 and 12 show 3-dimensional and schematic comparisons of substrate binding. The structures of NAM-NAG disaccharide in complex with AtlE and the NAGs complexes with ACOD provided the template for modeling of binding geometry of hexasaccharide substrates. The chain trace of the AtlE is shown on the background of the ACOD surface and vice versa, while the substrate models correspond to the structures with the shown surface. The pairs of FIGS. 11a and 11b as well as 12 a and 12 b demonstrate the differences between the shape of active site clefts and the way hexasaccharide substrates bind into them. In the AtlE structure the active site is broad, which makes the whole substrate model visible along the whole length. This is in sharp contrast to the narrow active site of ACOD which surface in part obscures the view of the substrate model. Since NAG and NAM residues appear at alternating positions, the lactic moieties are in the AtlE and ACOD models on the left and right side of the active site, respectively. This positioning indicates that features on the left side of the active site cleft of AtlE are responsible for recognition of lactyl moieties and peptides from the glycopeptide network, whereas the features on the right side of the active site cleft of AtlE could enhance rejection or even prevent binding of lactyl moieties and peptides attached to them. The reverse is expected for the ACOD substrate binding. Indeed the FIGS. 11a and 11b show that at and above the −2 and +1 positions of lactyl moieties of NAM residues bound to the AtlE surface there is enough space to accommodate the peptidyl extensions. They can surround the D175-K175 helical region which builds the top of the L-lobe. However, in the ACOD structure, there are features protruding outside the AtlE surface that can prevent binding of peptidyl extensions attached at these two positions. In accordance with the FIGS. 12a and 12b the reverse is true for the ACOD bound substrate model. The AtlE hairpin region from G52 to N68 of AtlE positioned at top of the R-lobe of the AtlE structure builds the wall of the active site on the right and thereby prevents binding of peptidyl extensions attached to lactyl group of NAM residues, whereas the lower ACOD surface provides sufficient space to accommodate peptides bound to the lactyl groups of NAM residues. This analysis demonstrates that the L- and R-lobes indeed contain structural features responsible for acceptance and rejection of the peptidyl moiety of glycopeptide cell wall.

Next, the selectivity against the saccharide (NAG-NAM)_(n) and (NAG)_(n) substrates with no peptidyl extensions attached were explored. In the substrate binding corresponding to muramidase activity, the lactyl group is positioned in −3, −1, and +2 subsites, whereas in the substrate binding corresponding to the glucosaminidase activity, the lactyl group of N-acetylmuramic acid is positioned in −2, +1, and +3 subsites. Clearly, there is no difference between the glycosidic bonds between the two carbohydrate rings, yet the muramidases cleave the glycosidic bond between O1 NAM-C4 NAG, whereas the glucosaminidases cleave the glycosidic bond between O1 NAG-C4 NAM. In addition, it is known that lysozyme/muramidases can cleave the glycosidic bond between two NAG consecutive residues too, whereas the AtlE and glu-AtlA cannot. In order to get further insight, the structural features responsible for acceptance and rejection of the lactyl moieties of the NAM residues were explored. In PDB there are several entries containing NAM among them are a few in complex with a hydrolase active site related to peptidoglycan substrate recognition. There are the complexes of NAM-peptide intermediate with T4 phage lysozyme (148 L) and NAM-NAG-NAM in the complex with the chicken lysozyme (9LYZ). In either case the lactyl moiety is not stabilized by any interaction with the underlying enzyme structure, whereas the N atom of the amide link in alanine in phage lysozyme, is oriented against the main chain carbonyl group of Q105. This indicates that lysozymes select the side of NAG-NAM polymers by excluding approach of the lactyl moiety from the wrong side, but do not require it at the other. This also explains why lysozymes can cleave also the NAG polymers. The AtlE NAG-NAM complex structure presented here, however, revealed that the lactate group of NAM -2 residue forms a hydrogen bond with Y201 (FIG. 6) and leaving enough space behind it to accommodate the peptidyl moiety (FIG. 7). The recognition of NAM residue leads to twist of the NAG-NAM chain at the −3 position. The absence of AtlE activity against the NAGs substrate can be attributed to the absence of the hydrogen bond between the lactyl moiety of −2 residue and Y201 and the possibly extended, but not twisted, conformation of the NAGs substrate, which disables productive binding at the −3 and −2 positions.

Taken together, the structures of AtlE and lyzozyme complexes revealed specific structural features which exclude the binding of the substrate molecules in the incompatible manner and thereby explain the difference between the glucosaminidase and muramidase activity. Since the peptidoglycan substrate is the same, in order to achieve the different binding, each kind of enzyme must approach the substrate molecules from a different side. This binding of the glycan reflects the differences in the chemistry of muramidases and glucosaminidases. The glycans form extended structures with carbohydrate rings are in the chair confirmation. In schematic figures, this important detail may easily escapes attention, however, observation of a 3-dimensional model revealed it according to the present invention. When viewed from a side, a zig-zag structure was found. Namely, the β-glycosidic bonds are separated by five of covalent bonds. The odd number of bonds brings the O4 atom in alternating positions, whether each second points either up or down (FIG. 13). If the glucosaminidases recognized the muramic moieties on the same side as the muramidases, then the catalytic residue from the bottom would not be able to reach the glycosidic bond oxygen atom positioned at the top. Therefore, they must approach from the opposite side, where the O4 atom from the glycosydic bond of the next pair of residues is accessible to the catalytic residue of the enzyme. Consequently, glucosaminidases contain structural features which accept lactate moieties on NAM residues on the R-side of the active site cleft contrary to muramidases. The absence of selective recognition of lactyl group on the L-side, however, enables them to process NAG polymers as well.

Hence, the combined analysis of the AtlE and lyzozyme structures revealed the features that define the glucosaminidase and muramidase activities of the enzymes.

This is achieved at two levels: At the first, their folds enable desired and prevent undesired binding of peptidyl parts of glycopeptidyl substrate. At the second, the detail architecture of the active site takes care for productive binding of the correct glycosidic bond adjusted by the lactyl moieties of NAM residues and approach of the O4 atoms in NAG-NAM polymers to the catalytic carboxylic group.

In the search for potential new targets for antibiotics for treatment of Staphylococcus aureus infections, the present invention shows the basic difference in substrate recognition between muramidases, which are one of the most studied enzymes, and glucosaminidases. These two groups of enzymes cleave alternate glycosidic bonds between NAG-NAM residues in a peptidoglycan structures comprising the bacterial cell wall. The difference between the enzymes is a consequence of the structure of NAG-NAM polymers in which only every second glycosidic oxygen atom is positioned at the same side of the polymer. In order to be able to reach alternate oxygen atoms, muramidases and glucosaminidases must dock to the substrate from the opposite sides. They achieve this by differences in the fold enhanced by specific interactions as revealed by the crystal structural analysis of autolysin E and its complexes with NAG-NAM and muropeptide. This gives one the opportunity to develop species specific antibiotics targeting cell wall degradation.

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TABLE 1 Structure and Refinement Statistics NAG-NAM NAM-NAG E138A Muropeptide AtlE AtlE complex ATlE complex AtlE complex PDB ID 4PIA 4PI7 4PI8 4PI9 Data Collection Statistics Resolution Range (Å) 23.0-1.47 50.0-1.6  38.4-1.39 38.75-1.48  (1.52-1.47) (1.69-1.60) (1.439-1.39)  (1.53-1.48) Space Group P 2₁ 2₁ 2₁ P 2₁ 2₁ 2₁ P 2₁ 2₁ 2₁ P 2₁ 2₁ 2₁ Unit Cell (Å) 46.60, 69.93, 46.31, 69.78, 46.011 69.72 45.63, 69.31, 73.27 73.58 73.54 73.42 90°, 90°, 90° 90°, 90°, 90° 90°, 90°, 90° 90°, 90°, 90° Total Reflections 229540 222199 312334 251936 Unique Reflections 41472 (3953) 31914 (2985) 48332 (4753) 39606 (3756) Multiplicity  5.5 (3.7)  3.7 (1.9)  6.5 (6.5)  6.4 (6.5) Completeness (%)  99.30 (95.97)  99.45 (94.82)  99.92 (99.69)  99.55 (96.26) Mean I/Sigma(I) 39.2 (1.4) 24.24 (3.34) 26.91 (3.00) 20.16 (2.07) Wilson B-Factor 11.72 16.14 15.96 19.66 R-Merge  0.043 (0.259)  12.1 (23.4) 0.03446 (0.5927) 0.04472 (0.7462) Refinement Statistic R-Work 0.1492 0.1563 0.152 0.1772 R-Free 0.1715 0.1868 0.1755 0.208 Number of Non-Hydrogen 2111 2116 2146 2111 Atoms Macromolecules 1844 1826 1837 1832 Ligands 9 50 53 42 Water 258 240 256 237 Protein Residues 225 223 222 223 RMS Bonds (Å) 0.017 0.015 0.018 0.015 RMS Angles (°) 1.81 1.64 1.9 1.7

TABLE 2 X-RAY DIFFRACTION DATA OF S. AUREUS AUTOLYSIN E (AtlE 4PIA) RESIDUES 35-258 Remarks REMARK 2 RESOLUTION: 1.47 ANGSTROMS. REMARK 3 PROGRAM: REFMAC REMARK 3 RESOLUTION RANGE HIGH (ANGSTROMS): 1.47 REMARK 3 RESOLUTION RANGE LOW (ANGSTROMS): 23.00 REMARK 3 DATA CUTOFF (SIGMA(F)): 0.000 REMARK 3 COMPLETENESS FOR RANGE (%): 99.3 REMARK 3 NUMBER OF REFLECTIONS: 41472 REMARK 3 FIT TO DATA USED IN REFINEMENT. REMARK 3 CROSS-VALIDATION METHOD: THROUGHOUT REMARK 3 FREE R VALUE TEST SET SELECTION: RANDOM REMARK 3 R VALUE (WORKING + TEST SET): 0.150 REMARK 3 R VALUE (WORKING SET): 0.149 REMARK 3 FREE R VALUE: 0.172 REMARK 3 FREE R VALUE TEST SET SIZE (%): 5.000 REMARK 3 FREE R VALUE TEST SET COUNT: 2090 REMARK 3 NUMBER OF NON-HYDROGEN ATOMS USED IN REFINEMENT. REMARK 3 PROTEIN ATOMS: 1809 REMARK 3 NUCLEIC ACID ATOMS: 0 REMARK 3 HETEROGEN ATOMS: 9 REMARK 3 SOLVENT ATOMS: 258 REMARK 3 B VALUES. REMARK 3 FROM WILSON PLOT (A**2): NULL REMARK 3 MEAN B VALUE (OVERALL, A**2): 15.92 REMARK 3 OVERALL ANISOTROPIC B VALUE. REMARK 3 B11 (A**2): 0.04000 REMARK 3 B22 (A**2): −0.02000 REMARK 3 B33 (A**2): −0.02000 REMARK 3 B12 (A**2): 0.00000 REMARK 3 B13 (A**2): 0.00000 REMARK 3 B23 (A**2): 0.00000 REMARK 3 ESTIMATED OVERALL COORDINATE ERROR. REMARK 3 ESU BASED ON R VALUE (A): 0.058 REMARK 3 ESU BASED ON FREE R VALUE (A): 0.059 REMARK 3 ESU BASED ON MAXIMUM LIKELIHOOD (A): 0.034 REMARK 3 ESU FOR B VALUES BASED ON MAXIMUM LIKELIHOOD (A**2): 0.862 REMARK 3 RMS DEVIATIONS FROM IDEAL VALUES. REMARK 3 DISTANCE RESTRAINTS. RMS SIGMA REMARK 3 BOND LENGTH (A): NULL; NULL REMARK 3 ANGLE DISTANCE (A): NULL; NULL REMARK 3 INTRAPLANAR 1-4 DISTANCE (A): NULL; NULL REMARK 3 H-BOND OR METAL COORDINATION (A): NULL; NULL REMARK 3 PLANE RESTRAINT (A): NULL; NULL REMARK 3 CHIRAL-CENTER RESTRAINT (A**3): NULL; NULL REMARK 3 NON-BONDED CONTACT RESTRAINTS. REMARK 3 SINGLE TORSION (A): NULL; NULL REMARK 3 MULTIPLE TORSION (A): NULL; NULL REMARK 3 H-BOND (X . . . Y) (A): NULL; NULL REMARK 3 H-BOND (X-H . . . Y) (A): NULL; NULL REMARK 3 CONFORMATIONAL TORSION ANGLE RESTRAINTS. REMARK 3 SPECIFIED (DEGREES): NULL; NULL REMARK 3 PLANAR (DEGREES): NULL; NULL REMARK 3 STAGGERED (DEGREES): NULL; NULL REMARK 3 TRANSVERSE (DEGREES): NULL; NULL REMARK 3 ISOTROPIC THERMAL FACTOR RESTRAINTS. RMS SIGMA REMARK 3 MAIN-CHAIN BOND (A**2): NULL; NULL REMARK 3 MAIN-CHAIN ANGLE (A**2): NULL; NULL REMARK 3 SIDE-CHAIN BOND (A**2): NULL; NULL REMARK 3 SIDE-CHAIN ANGLE (A**2): NULL; NULL REMARK 3 OTHER REFINEMENT REMARKS: HYDROGENS HAVE BEEN ADDED IN THE RIDING REMARK 3 POSITIONS U VALUES: REFINED INDIVIDUALLY REMARK 4 4PIA COMPLIES WITH FORMAT V. 3.30, 13-JUL-11 REMARK 200 EXPERIMENTAL DETAILS REMARK 200 EXPERIMENT TYPE: X-RAY DIFFRACTION REMARK 200 TEMPERATURE (KELVIN): 100 REMARK 200 PH: NULL REMARK 200 NUMBER OF CRYSTALS USED: 1 REMARK 200 SYNCHROTRON (Y/N): Y REMARK 200 RADIATION SOURCE: ELETTRA REMARK 200 BEAMLINE: 5.2R REMARK 200 X-RAY GENERATOR MODEL: NULL REMARK 200 MONOCHROMATIC OR LAUE (M/L): M REMARK 200 WAVELENGTH OR RANGE (A): 1.00 REMARK 200 MONOCHROMATOR: NULL REMARK 200 OPTICS: NULL REMARK 200 DETECTOR TYPE: PIXEL REMARK 200 DETECTOR MANUFACTURER: DECTRIS PILATUS 6M REMARK 200 INTENSITY-INTEGRATION SOFTWARE: HKL REMARK 200 DATA SCALING SOFTWARE: HKL REMARK 200 NUMBER OF UNIQUE REFLECTIONS: 41563 REMARK 200 RESOLUTION RANGE HIGH (A): 1.460 REMARK 200 RESOLUTION RANGE LOW (A): 23.000 REMARK 200 REJECTION CRITERIA (SIGMA(I)): NULL REMARK 200 OVERALL. REMARK 200 COMPLETENESS FOR RANGE (%): 99.3 REMARK 200 DATA REDUNDANCY: 5.500 REMARK 200 R MERGE (I): 0.04300 REMARK 200 R SYM (I): NULL REMARK 200 <I/SIGMA(I)> FOR THE DATA SET: 39.2000 REMARK 200 IN THE HIGHEST RESOLUTION SHELL. REMARK 200 HIGHEST RESOLUTION SHELL, RANGE HIGH (A): 1.46 REMARK 200 HIGHEST RESOLUTION SHELL, RANGE LOW (A): 1.49 REMARK 200 COMPLETENESS FOR SHELL (%): 96.0 REMARK 200 DATA REDUNDANCY IN SHELL: 4.00 REMARK 200 R MERGE FOR SHELL (I): 0.29100 REMARK 200 R SYM FOR SHELL (I): NULL REMARK 200 <I/SIGMA(I)> FOR SHELL: NULL REMARK 200 DIFFRACTION PROTOCOL: SINGLE WAVELENGTH REMARK 200 METHOD USED TO DETERMINE THE STRUCTURE: NULL REMARK 200 SOFTWARE USED: NULL REMARK 200 STARTING MODEL: NULL REMARK 200 REMARK: NULL REMARK 280 CRYSTAL REMARK 280 SOLVENT CONTENT, VS (%): 46.28 REMARK 280 MATTHEWS COEFFICIENT, VM (ANGSTROMS**3/DA): 2.29 REMARK 280 CRYSTALLIZATION CONDITIONS: 2 M (NH4)2S04, 2 M NACL REMARK 290 CRYSTALLOGRAPHIC SYMMETRY REMARK 290 SYMMETRY OPERATORS FOR SPACE GROUP: P 21 21 21 REMARK 290 SYMOP SYMMETRY REMARK 290 NNNMMM OPERATOR REMARK 290 1555 X, Y, Z REMARK 290 2555 −X+1/2, −Y, Z+1/2 REMARK 290 3555 −X, Y+1/2, −Z+1/2 REMARK 290 4555 X+1/2, −Y+1/2, −Z REMARK 290 WHERE NNN -> OPERATOR NMBER REMARK 290 MMM -> TRANSLATION VECTOR REMARK 290 CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS REMARK 290 THE FOLLOWING TRANSFORMATIONS OPERATE ON THE ATOM/HETATM REMARK 290 RECORDS IN THIS ENTRY TO PRODUCE CRYSTALLOGRAPHICALLY REMARK 290 RELATED MOLECULES. REMARK 290 SMTRY1 1 1.000000 0.000000 0.000000 0.00000 REMARK 290 SMTRY2 1 0.000000 1.000000 0.000000 0.00000 REMARK 290 SMTRY3 1 0.000000 0.000000 1.000000 0.00000 REMARK 290 SMTRY1 2 −1.000000   0.000000 0.000000 23.29950  REMARK 290 SMTRY2 2 0.000000 −1.000000   0.000000 0.00000 REMARK 290 SMTRY3 2 0.000000 0.000000 1.000000 36.63400  REMARK 290 SMTRY1 3 −1.000000   0.000000 0.000000 0.00000 REMARK 290 SMTRY2 3 0.000000 1.000000 0.000000 34.96350  REMARK 290 SMTRY3 3 0.000000 0.000000 −1.000000   36.63400  REMARK 290 SMTRY1 4 1.000000 0.000000 0.000000 23.29950  REMARK 290 SMTRY2 4 0.000000 −1.000000   0.000000 34.96350  REMARK 290 SMTRY3 4 0.000000 0.000000 −1.000000   0.00000 REMARK 350 BIOLOGICAL UNIT: MONOMERIC REMARK 350 SOFTWARE DETERMINED QUATERNARY STRUCTURE: MONOMERIC REMARK 350 SOFTWARE USED: PISA REMARK 350 TOTAL BURIED SURFACE AREA: 980 ANGSTROM**2 REMARK 350 SURFACE AREA OF THE COMPLEX: 11270 ANGSTROM**2 REMARK 350 CHANGE IN SOLVENT FREE ENERGY: −71.0 KCAL/MOL REMARK 350 APPLY THE FOLLOWING TO CHAINS: A REMARK 350 BIOMT1 1 1.000000 0.000000 0.000000 0.00000 REMARK 350 BIOMT2 1 0.000000 1.000000 0.000000 0.00000 REMARK 350 BIOMT3 1 0.000000 0.000000 1.000000 0.00000 REMARK 465 MISSING RESIDUES REMARK 465 THE FOLLOWING RESIDUES WERE NOT LOCATED IN THE REMARK 465 EXPERIMENT. (M = MODEL NUMBER; RES = RESIDUE NAME; C = CHAIN REMARK 465 IDENTIFIER; SSSEQ = SEQUENCE NUMBER; I = INSERTION CODE.) REMARK 465 M RES C SSSEQ I REMARK 465 SER A 31 REMARK 465 HIS A 79 REMARK 465 LYS A 80 REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: CLOSE CONTACTS IN SAME ASYMMETRIC UNIT REMARK 500 THE FOLLOWING ATOMS ARE IN CLOSE CONTACT. REMARK 500 ATM1 RES C SSSEQ I ATM2 RES C SSEQ I DISTANCE REMARK 500 O HOH A 586 O HOH A 656 2.04 REMARK 500 O HOH A 651 O HOH A 657 2.08 REMARK 500 REMARK: NULL REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: CLOSE CONTACTS REMARK 500 THE FOLLOWING ATOMS THAT ARE RELATED BY CRYSTALLOGRAPHIC REMARK 500 SYMMETRY ARE IN CLOSE CONTACT. AN ATOM LOCATED WITHIN 0.15 REMARK 500 ANGSTROMS OF A SYMMETRY RELATED ATOM IS ASSUMED TO BE ON A REMARK 500 SPECIAL POSITION. ATOMS WITH NON-BLANK ALTERNATE REMARK 500 LOCATION INDICATORS ARE NOT INCLUDED IN THE CALCULATIONS. REMARK 500 DISTANCE CUTOFF: REMARK 500 2.2 ANGSTROMS FOR CONTACTS NOT INVOLVING HYDROGEN ATOMS REMARK 500 1.6 ANGSTROMS FOR CONTACTS INVOLVING HYDROGEN ATOMS REMARK 500 ATM1 RES C SSSEQ I ATM2 RES C SSEQI I SSYMOP DISTANCE REMARK 500 O HOH A 472 O HOH A 501 4445 1.98 REMARK 500 REMARK: NULL REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: COVALENT BOND ANGLES REMARK 500 THE STEREOCHEMICAL PARAMETERS OF THE FOLLOWING RESIDUES REMARK 500 HAVE VALUES WHICH DEVIATE FROM EXPECTED VALUES BY MORE REMARK 500 THAN 6*RMSD (M = MODEL NUMBER; RES = RESIDUE NAME; C = CHAIN REMARK 500 IDENTIFIER; SSEQ = SEQUENCE NUMBER; I = INSERTION CODE). REMARK 500 STANDARD TABLE: REMARK 500 FORMAT: (10X, I3, 1X, A3, 1X, A1, I4, A1, 3(1X, A4, 2X), 12X, F5.1) REMARK 500 EXPECTED VALUES PROTEIN: ENGH AND HUBER, 1999 REMARK 500 EXPECTED VALUES NUCLEIC ACID: CLOWNEY ET AL 1996 REMARK 500 M RES C SSEQ I ATM1 ATM2 ATM3 REMARK 500 MET A  84 CA — CB — CG ANGL. DEV. = 10.6 DEGREES REMARK 500 MET A  84 CG — SD — CE ANGL. DEV. = −13.6 DEGREES REMARK 500 ASP A  88 CB — CG — OD1 ANGL. DEV. = 6.5 DEGREES REMARK 500 ASP A 227 CB — CG — OD1 ANGL. DEV. = 6.5 DEGREES REMARK 500 ASP A 227 CB — CG — OD2 ANGL. DEV. = −7.0 DEGREES REMARK 500 REMARK: NULL REMARK 500 GEOMETRY AND STEREOCHEMISTRY REMARK 500 SUBTOPIC: TORSION ANGLES REMARK 500 TORSION ANGLES OUTSIDE THE EXPECTED RAMACHANDRAN REGIONS: REMARK 500 (M = MODEL NUMBER; RES = RESIDUE NAME; C = CHAIN IDENTIFIER; REMARK 500 SSEQ = SEQUENCE NUMBER; I = INSERTION CODE). REMARK 500 STANDARD TABLE: REMARK 500 FORMAT: (10X, I3, 1X, A3, 1X, A1, I4, A1, 4X, F7.2, 3X, F7.2) REMARK 500 EXPECTED VALUES: GJ KLEYWEGT AND TA JONES (1996). PHI/ REMARK 500 PSICHOLOGY: RAMACHANDRAN REVISITED. STRUCTURE 4, 1395-1400 REMARK 500 M RES C SSEQ I PSI PHI REMARK 500 ASN A 159 113.44 −160.04   REMARK 500 LYS A 175  44.96  −97.51   REMARK 500 ASN A 215  66.23  26.87 REMARK 500 ALA A 220  −4.25    79.83 REMARK 500 REMARK: NULL REMARK 800 SITE REMARK 800 SITE_IDENTIFIER: AC1 REMARK 800 EVIDENCE_CODE: SOFTWARE REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 301 REMARK 800 SITE_IDENTIFIER: AC2 REMARK 800 EVIDENCE_CODE: SOFTWARE REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 302 REMARK 800 SITE_IDENTIFIER: AC3 REMARK 800 EVIDENCE_CODE: SOFTWARE REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 303 REMARK 800 SITE_IDENTIFIER: AC4 REMARK 800 EVIDENCE_CODE: SOFTWARE REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 304 REMARK 800 SITE_IDENTIFIER: AC5 REMARK 800 EVIDENCE_CODE: SOFTWARE REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 305 REMARK 800 SITE_IDENTIFIER: AC6 REMARK 800 EVIDENCE_CODE: SOFTWARE REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 306 REMARK 800 SITE_IDENTIFIER: AC7 REMARK 800 EVIDENCE_CODE: SOFTWARE REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 307 REMARK 800 SITE_IDENTIFIER: AC8 REMARK 800 EVIDENCE_CODE: SOFTWARE REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 308 REMARK 800 SITE_IDENTIFIER: AC9 REMARK 800 EVIDENCE_CODE: SOFTWARE REMARK 800 SITE_DESCRIPTION: binding site for residue CL A 309 REMARK 900 RELATED ENTRIES REMARK 900 RELATED ID: 4PI8 RELATED DB: PDB REMARK 900 RELATED ID: 4PI7 RELATED DB: PDB REMARK 900 RELATED ID: 4PI9 RELATED DB: PDB DBREF 4PIA A  35 258 UNP Q99RW6 Q99RW6_STAAM 35 258 SEQADV 4PIA SER A  31 UNP Q99RW6 EXPRESSION TAG SEQADV 4PIA ALA A  32 UNP Q99RW6 EXPRESSION TAG SEQADV 4PIA ALA A  33 UNP Q99RW6 EXPRESSION TAG SEQADV 4PIA ALA A  34 UNP Q99RW6 EXPRESSION TAG SEQRES 1 A 228 SER ALA ALA ALA ASN ASP VAL ASN TYR SER PHE ASP GLU SEQRES 2 A 228 ALA VAL SER MET GLN GLN GLY LYS GLY ILE VAL GLN THR SEQRES 3 A 228 LYS GLU GLU ASP GLY LYS PHE VAL GLU ALA ASN ASN ASN SEQRES 4 A 228 GLU ILE ALA LYS ALA MET THR ILE SER HIS LYS ASP ASN SEQRES 5 A 228 ASP MET LYS TYR MET ASP ILE THR GLU LYS VAL PRO MET SEQRES 6 A 228 SER GLU SER GLU VAL ASN GLN LEU LEU LYS GLY LYS GLY SEQRES 7 A 228 ILE LEU GLU ASN ARG GLY LYS VAL PHE LEU GLU ALA GLN SEQRES 8 A 228 GLU LYS TYR GLU VAL ASN VAL ILE TYR LEU VAL SER HIS SEQRES 9 A 228 ALA LEU VAL GLU THR GLY ASN GLY LYS SER GLU LEU ALA SEQRES 10 A 228 LYS GLY ILE LYS ASP GLY LYS LYS ARG TYR TYR ASN PHE SEQRES 11 A 228 PHE GLY ILE GLY ALA PHE ASP SER SER ALA VAL ARG SER SEQRES 12 A 228 GLY LYS SER TYR ALA GLU LYS GLU GLN TRP THR SER PRO SEQRES 13 A 228 ASP LYS ALA ILE ILE GLY GLY ALA LYS PHE ILE ARG ASN SEQRES 14 A 228 GLU TYR PHE GLU ASN ASN GLN LEU ASN LEU TYR GLN MET SEQRES 15 A 228 ARG TRP ASN PRO GLU ASN PRO ALA GLN HIS GLN TYR ALA SEQRES 16 A 228 SER ASP ILE ARG TRP ALA ASP LYS ILE ALA LYS LEU MET SEQRES 17 A 228 ASP LYS SER TYR LYS GLN PHE GLY ILE LYS LYS ASP ASP SEQRES 18 A 228 ILE ARG GLN THR TYR TYR LYS HET CL A 301  1 HET CL A 302  1 HET CL A 303  1 HET CL A 304  1 HET CL A 305  1 HET CL A 306  1 HET CL A 307  1 HET CL A 308  1 HET CL A 309  1 HETNAM CL CHLORIDE ION FORMUL 2 CL 9 (CL 1−) FORMUL 11 HOH *258(H2 O) HELIX 1 AA1 SER A  40 GLN A  49 1  10 HELIX 2 AA2 ASN A  67 THR A  76 1  10 HELIX 3 AA3 ASP A  83 MET A  87 5  5 HELIX 4 AA4 SER A  96 LYS A 105 1  10 HELIX 5 AA5 LYS A 107 GLU A 111 5  5 HELIX 6 AA6 ARG A 113 GLU A 125 1  13 HELIX 7 AA7 ASN A 127 THR A 139 1  13 HELIX 8 AA8 GLU A 145 GLY A 149 5  5 HELIX 9 AA9 ASP A 167 GLY A 174 1  8 HELIX 10 AB1 SER A 176 GLU A 181 1  6 HELIX 11 AB2 SER A 185 TYR A 201 1  17 HELIX 12 AB3 PHE A 202 ASN A 205 5  4 HELIX 13 AB4 ASN A 208 ASN A 215 1  8 HELIX 14 AB5 ARG A 229 GLY A 246 1  18 SHEET 1 AA1  2 THE A  56 GLU A  59 0 SHEET 2 AA1  2 LYS A  62 GLU A  65 −1   O LYS A  62 N GLU A  59 SHEET 1 AA2  2 ILE A 150 ASP A 152 0 SHEET 2 AA2  2 LYS A 155 TYR A 157 −1   O TYR A 157 N ILE A 150 SITE 1 AC1  2 TYR A 256 TYR A 257 SITE 1 AC2  4 ASN A 204 ASN A 215 HOH A 434 HOH A 618 SITE 1 AC3  6 GLY A 106 GLY A 108 LYS A 143 LYS A 243 SITE 2 AC3  6 HOH A 472 HOH A 508 SITE 1 AC4  4 GLY A 164 ALA A 165 LYS A 175 TYR A 177 SITE 1 AC5  1 GLU A 145 SITE 1 AC6  4 PRO A  94 LYS A 248 HOH A 585 HOH A 590 SITE 1 AC7  6 SER A  96 GLU A  97 SER A  98 ASN A 218 SITE 2 AC7  6 HOH A 407 HOH A 504 SITE 1 AC8  3 ALA A  74 LYS A  85 TRP A 214 SITE 1 AC9  6 ASN A 101 ASN A 112 GLY A 114 LYS A 115 SITE 2 AC9  6 HOH A 491 HOH A 492 CRYST1 46.599 69.927 73.268 90.00 90.00 90.00 P 21 21 21  4 ORIGX1 1.000000 0.000000 0.000000 0.00000 ORIGX2 0.000000 1.000000 0.000000 0.00000 ORIGX3 0.000000 0.000000 1.000000 0.00000 SCALE1 0.021460 0.000000 0.000000 0.00000 SCALE2 0.000000 0.014301 0.000000 0.00000 SCALE3 0.000000 0.000000 0.013649 0.00000 Data ATOM 1 N ALA A 32 2.887 −1.791 −39.094 1.00 52.61 N ATOM 2 CA ALA A 32 4.235 −1.614 −39.730 1.00 49.15 C ATOM 3 C ALA A 32 5.336 −1.323 −38.692 1.00 46.23 C ATOM 4 O ALA A 32 6.239 −2.145 −38.508 1.00 44.29 O ATOM 5 CB ALA A 32 4.194 −0.529 −40.802 1.00 52.21 C ATOM 6 N ALA A 33 5.274 −0.169 −38.013 1.00 42.62 N ATOM 7 CA ALA A 33 6.328 0.201 −37.048 1.00 40.01 C ATOM 8 C ALA A 33 6.082 −0.407 −35.654 1.00 38.69 C ATOM 9 O ALA A 33 5.132 −0.019 −34.957 1.00 38.26 O ATOM 10 CB ALA A 33 6.479 1.709 −36.946 1.00 40.18 C ATOM 11 N ALA A 34 6.952 −1.345 −35.263 1.00 35.68 N ATOM 12 CA ALA A 34 6.908 −1.989 −33.939 1.00 36.70 C ATOM 13 C ALA A 34 8.095 −1.506 −33.075 1.00 32.89 C ATOM 14 O ALA A 34 8.540 −2.179 −32.106 1.00 36.83 O ATOM 15 CB ALA A 34 6.938 −3.503 −34.097 1.00 34.69 C ATOM 16 N ASN A 35 8.606 −0.331 −33.423 1.00 28.59 N ATOM 17 CA ASN A 35 9.714 0.230 −32.714 1.00 24.48 C ATOM 18 C ASN A 35 9.220 1.141 −31.587 1.00 21.91 C ATOM 19 O ASN A 35 8.793 2.287 −31.799 1.00 23.98 O ATOM 20 CB ASN A 35 10.583 1.015 −33.663 1.00 28.59 C ATOM 21 CG ASN A 35 11.907 1.426 −33.050 1.00 30.14 C ATOM 22 OD1 ASN A 35 12.023 1.644 −31.856 1.00 27.37 O ATOM 23 ND2 ASN A 35 12.912 1.535 −33.879 1.00 34.04 N ATOM 24 N ASP A 36 9.360 0.621 −30.372 1.00 15.66 N ATOM 25 CA ASP A 36 8.920 1.327 −29.171 1.00 14.24 C ATOM 26 C ASP A 36 10.060 1.994 −28.420 1.00 12.82 C ATOM 27 O ASP A 36 9.899 2.435 −27.272 1.00 12.99 O ATOM 28 CB ASP A 36 8.235 0.366 −28.217 1.00 16.30 C ATOM 29 CG ASP A 36 9.043 −0.891 −27.967 1.00 19.31 C ATOM 30 OD1 ASP A 36 10.238 −0.877 −28.264 1.00 15.46 O ATOM 31 OD2 ASP A 36 8.495 −1.963 −27.585 1.00 24.87 O ATOM 32 N VAL A 37 11.238 2.077 −29.041 1.00 12.02 N ATOM 33 CA VAL A 37 12.370 2.770 −28.431 1.00 11.68 C ATOM 34 C VAL A 37 12.322 4.211 −28.940 1.00 13.11 C ATOM 35 O VAL A 37 12.753 4.522 −30.062 1.00 14.64 O ATOM 36 CB VAL A 37 13.710 2.058 −28.709 1.00 12.19 C ATOM 37 CG1 VAL A 37 14.890 2.847 −28.147 1.00 12.91 C ATOM 38 CG2 VAL A 37 13.679 0.643 −28.176 1.00 12.08 C ATOM 39 N ASN A 38 11.798 5.089 −28.115 1.00 10.08 N ATOM 40 CA ASN A 38 11.456 6.451 −28.507 1.00 9.90 C ATOM 41 C ASN A 38 12.299 7.552 −27.925 1.00 9.85 C ATOM 42 O ASN A 38 12.394 8.656 −28.516 1.00 11.43 O ATOM 43 CB ASN A 38 10.021 6.703 −28.061 1.00 9.86 C ATOM 44 CG ASN A 38 9.017 6.051 −28.943 1.00 12.62 C ATOM 45 OD1 ASN A 38 9.297 5.717 −30.127 1.00 13.95 O ATOM 46 ND2 ASN A 38 7.824 5.792 −28.398 1.00 12.04 N ATOM 47 N TYR A 39 12.932 7.339 −26.774 1.00 8.55 N ATOM 48 CA TYR A 39 13.623 8.403 −25.993 1.00 8.56 C ATOM 49 C TYR A 39 15.004 7.934 −25.567 1.00 9.75 C ATOM 50 O TYR A 39 15.227 6.746 −25.341 1.00 9.87 O ATOM 51 CB TYR A 39 12.822 8.778 −24.736 1.00 8.95 C ATOM 52 CG TYR A 39 11.535 9.444 −25.069 1.00 8.84 C ATOM 53 CD1 TYR A 39 11.519 10.816 −25.344 1.00 9.00 C ATOM 54 CD2 TYR A 39 10.346 8.764 −25.187 1.00 8.99 C ATOM 55 CE1 TYR A 39 10.351 11.453 −25.652 1.00 9.97 C ATOM 56 CE2 TYR A 39 9.171 9.416 −25.535 1.00 8.56 C ATOM 57 CZ TYR A 39 9.181 10.766 −25.768 1.00 8.90 C ATOM 58 OH TYR A 39 8.003 11.416 −26.091 1.00 9.11 O ATOM 59 N SER A 40 15.949 8.857 −25.491 1.00 10.73 N ATOM 60 CA SER A 40 17.183 8.598 −24.787 1.00 11.27 C ATOM 61 C SER A 40 16.920 8.453 −23.294 1.00 10.90 C ATOM 62 O SER A 40 15.887 8.939 −22.747 1.00 10.82 O ATOM 63 CB SER A 40 18.218 9.712 −24.981 1.00 12.11 C ATOM 64 OG SER A 40 17.803 10.858 −24.297 1.00 13.09 O ATOM 65 N PHE A 41 17.852 7.800 −22.601 1.00 10.57 N ATOM 66 CA PHE A 41 17.707 7.718 −21.147 1.00 10.64 C ATOM 67 C PHE A 41 17.603 9.056 −20.497 1.00 11.38 C ATOM 68 O PHE A 41 16.739 9.312 −19.669 1.00 11.96 O ATOM 69 CB PHE A 41 18.874 6.853 −20.562 1.00 10.89 C ATOM 40 CG PHE A 41 18.879 6.632 −19.100 1.00 11.85 C ATOM 71 CD1 PHE A 41 17.831 5.808 −18.569 1.00 12.56 C ATOM 72 CD2 PHE A 41 19.610 7.290 −18.228 1.00 13.16 C ATOM 73 CE1 PHE A 41 17.747 5.564 −17.224 1.00 14.15 C ATOM 74 CE2 PHE A 41 19.478 7.077 −16.861 1.00 13.79 C ATOM 75 CZ PHE A 41 18.566 6.185 −16.368 1.00 14.56 C ATOM 76 N ASP A 42 18.481 9.987 −20.886 1.00 12.27 N ATOM 77 CA ASP A 42 18.464 11.302 −20.267 1.00 13.54 C ATOM 78 C ASP A 42 17.168 12.071 −20.525 1.00 11.93 C ATOM 79 O ASP A 42 16.652 12.750 −19.602 1.00 12.11 O ATOM 80 CB ASP A 42 19.643 12.1418 −20.774 1.00 16.27 C ATOM 81 CG ASP A 42 20.980 11.720 −20.189 1.00 20.56 C ATOM 82 OD1 ASP A 42 21.026 10.927 −19.245 1.00 22.33 O ATOM 83 OD2 ASP A 42 21.984 12.249 −20.768 1.00 24.00 O ATOM 84 N GLU A 43 16.581 11.897 −21.726 1.00 11.06 N ATOM 85 CA GLU A 43 15.276 12.512 −21.990 1.00 11.31 C ATOM 86 C GLU A 43 14.187 11.929 −21.072 1.00 9.74 C ATOM 87 O GLU A 43 13.403 12.648 −20.482 1.00 10.57 O ATOM 88 CB GLU A 43 14.866 12.302 −23.427 1.00 12.74 C ATOM 89 CG GLU A 43 15.655 13.133 −24.454 1.00 13.82 C ATOM 90 CD GLU A 43 15.566 12.628 −25.891 1.00 17.54 C ATOM 91 OE1 GLU A 43 15.094 11.519 −26.278 1.00 13.79 O ATOM 92 OE2 GLU A 43 16.008 13.467 −26.765 1.00 22.32 O ATOM 93 N ALA A 44 14.186 10.602 −20.953 1.00 9.66 N ATOM 94 CA ALA A 44 13.213 9.949 −20.071 1.00 9.03 C ATOM 95 C ALA A 44 13.338 10.334 −18.624 1.00 8.59 C ATOM 96 O ALA A 44 12.363 10.586 −17.909 1.00 8.73 O ATOM 97 CB ALA A 44 13.318 8.459 −20.227 1.00 9.61 C ATOM 98 N VAL A 45 14.590 10.423 −18.159 1.00 8.86 N ATOM 99 CA VAL A 45 14.823 10.881 −16.799 1.00 9.57 C ATOM 100 C VAL A 45 14.234 12.271 −16.555 1.00 9.28 C ATOM 101 O VAL A 45 13.540 12.506 −15.554 1.00 9.51 O ATOM 102 CB VAL A 45 16.358 10.893 −16.451 1.00 10.76 C ATOM 103 CG1 VAL A 45 16.606 11.590 −15.145 1.00 11.02 C ATOM 104 CG2 VAL A 45 16.903 9.484 −16.423 1.00 10.84 C ATOM 105 N SER A 46 14.489 13.203 −17.479 1.00 9.68 N ATOM 106 CA ASER A 46 13.941 14.529 −17.301 0.70 10.81 C ATOM 107 CA BSER A 46 13.937 14.551 −17.358 0.30 9.87 C ATOM 108 C SER A 46 12.410 14.535 −17.225 1.00 9.50 C ATOM 109 O SER A 46 11.829 15.276 −16.463 1.00 9.71 O ATOM 110 CB ASER A 46 14.395 15.380 −18.461 0.70 12.52 C ATOM 111 CB BSER A 46 14.355 15.368 −18.581 0.30 10.33 C ATOM 112 OG ASER A 46 14.031 16.720 −18.193 0.70 16.54 O ATOM 113 OG BSER A 46 15.692 15.822 −18.439 0.30 11.11 O ATOM 114 N MET A 47 11.762 13.675 −18.017 1.00 8.43 N ATOM 115 CA MET A 47 10.316 13.526 −17.974 1.00 8.20 C ATOM 116 C MET A 47 9.857 12.983 −16.616 1.00 8.00 C ATOM 117 O MET A 47 8.908 13.474 −16.068 1.00 8.28 O ATOM 118 CB MET A 47 9.846 12.668 −19.155 1.00 8.58 C ATOM 119 CG MET A 47 10.122 13.339 −20.477 1.00 8.66 C ATOM 120 SD MET A 47 10.017 12.315 −21.963 1.00 9.33 S ATOM 121 CE MET A 47 8.234 12.156 −22.121 1.00 10.28 C ATOM 122 N GLN A 48 10.531 11.932 −16.122 1.00 7.77 N ATOM 123 CA GLN A 48 10.150 11.365 −14.836 1.00 8.11 C ATOM 124 C GLN A 48 10.335 12.350 −13.674 1.00 8.44 C ATOM 125 O GLN A 48 9.635 12.252 −12.674 1.00 9.07 O ATOM 126 CB GLN A 48 10.995 10.131 −14.559 1.00 8.23 C ATOM 127 CG GLN A 48 10.863 8.984 −15.534 1.00 7.89 C ATOM 128 CD GLN A 48 9.542 8.256 −15.495 1.00 7.63 C ATOM 129 OE1 GLN A 48 8.694 8.485 −14.636 1.00 8.36 O ATOM 130 NE2 GLN A 48 9.394 7.327 −16.390 1.00 7.67 N ATOM 131 N GLN A 49 11.238 13.319 −13.867 1.00 8.69 N ATOM 132 CA GLN A 49 11.489 14.384 −12.900 1.00 9.70 C ATOM 133 C GLN A 49 10.625 15.611 −13.071 1.00 10.38 C ATOM 134 O GLN A 49 10.811 16.655 −12.399 1.00 11.00 O ATOM 135 CB GLN A 49 12.947 14.784 −12.971 1.00 9.65 C ATOM 136 CG GLN A 49 13.910 13.676 −12.533 1.00 11.05 C ATOM 137 CD GLN A 49 15.359 13.943 −12.881 1.00 12.64 C ATOM 138 OE1 GLN A 49 15.681 14.830 −13.684 1.00 14.24 O ATOM 139 NE2 GLN A 49 16.262 13.170 −12.260 1.00 13.39 N ATOM 140 N GLY A 50 9.659 15.531 −13.987 1.00 8.85 N ATOM 141 CA GLY A 50 8.893 16.691 −14.366 1.00 9.43 C ATOM 142 C GLY A 50 8.027 17.284 −13.314 1.00 10.06 C ATOM 143 O GLY A 50 7.745 16.671 −12.239 1.00 11.24 O ATOM 144 N LYS A 51 7.477 18.474 −13.597 1.00 11.01 N ATOM 145 CA LYS A 51 6.716 19.214 −12.596 1.00 13.93 C ATOM 146 C LYS A 51 5.512 18.484 −12.070 1.00 13.99 C ATOM 147 O LYS A 51 4.687 17.983 −12.795 1.00 13.21 O ATOM 148 CB LYS A 51 6.269 20.563 −13.143 1.00 16.02 C ATOM 149 CG LYS A 51 5.512 21.304 −12.035 1.00 19.92 C ATOM 150 CD LYS A 51 5.204 22.720 −12.408 1.00 22.96 C ATOM 151 CE LYS A 51 4.351 23.379 −11.326 1.00 22.39 C ATOM 152 NZ LYS A 51 5.139 23.650 −10.079 1.00 27.37 N ATOM 153 N GLY A 52 5.427 18.396 −10.733 1.00 15.93 N ATOM 154 CA GLY A 52 4.272 17.790 −10.120 1.00 17.91 C ATOM 155 C GLY A 52 4.183 16.282 −10.105 1.00 18.74 C ATOM 156 O GLY A 52 3.207 15.725 −9.629 1.00 22.96 O ATOM 157 N ILE A 53 5.164 15.585 −10.677 1.00 15.59 N ATOM 158 CA ILE A 53 5.096 14.158 −10.793 1.00 16.44 C ATOM 159 C ILE A 53 5.571 13.497 −9.493 1.00 18.63 C ATOM 160 O ILE A 53 6.711 13.701 −9.055 1.00 19.29 O ATOM 161 CB ILE A 53 5.955 13.684 −11.942 1.00 15.44 C ATOM 162 CG1 ILE A 53 5.339 14.170 −13.272 1.00 15.04 C ATOM 163 CG2 ILE A 53 6.042 12.159 −11.990 1.00 17.43 C ATOM 164 CD1 ILE A 53 6.198 13.874 −14.452 1.00 14.22 C ATOM 165 N VAL A 54 4.704 12.668 −8.938 1.00 17.27 N ATOM 166 CA VAL A 54 4.948 12.024 −7.624 1.00 18.81 C ATOM 167 C VAL A 54 5.719 10.735 −7.783 1.00 16.27 C ATOM 168 O VAL A 54 5.285 9.762 −8.459 1.00 17.86 O ATOM 169 CB VAL A 54 3.608 11.740 −6.905 1.00 20.19 C ATOM 170 CG1 VAL A 54 3.811 10.951 −5.593 1.00 20.75 C ATOM 171 CG2 VAL A 54 2.888 13.063 −6.674 1.00 23.17 C ATOM 172 N GLN A 55 6.917 10.761 −7.248 1.00 13.30 N ATOM 173 CA GLN A 55 7.753 9.583 −7.207 1.00 12.60 C ATOM 174 C GLN A 55 8.144 9.478 −5.750 1.00 12.95 C ATOM 175 O GLN A 55 8.674 10.434 −5.177 1.00 14.01 O ATOM 176 CB GLN A 55 8.963 9.698 −8.107 1.00 12.83 C ATOM 177 CG GLN A 55 8.700 10.232 −9.545 1.00 13.00 C ATOM 178 CD GLN A 55 8.564 9.211 −10.665 1.00 12.55 C ATOM 179 OE1 GLN A 55 8.677 9.567 −11.871 1.00 11.92 O ATOM 180 NE2 GLN A 55 8.333 8.025 −10.367 1.00 11.21 N ATOM 181 N THR A 56 7.961 8.285 −5.175 1.00 12.32 N ATOM 182 CA THR A 56 8.133 8.082 −3.720 1.00 12.74 C ATOM 183 C THR A 56 9.146 7.005 −3.371 1.00 14.34 C ATOM 184 O THR A 56 9.503 6.152 −4.195 1.00 12.85 O ATOM 185 CB THR A 56 6.844 7.817 −3.043 1.00 13.72 C ATOM 186 OG1 THR A 56 6.235 6.619 −3.549 1.00 13.52 O ATOM 187 CG2 THR A 56 5.898 9.023 −3.203 1.00 15.37 C ATOM 188 N LYS A 57 9.635 7.061 −2.120 1.00 15.28 N ATOM 189 CA LYS A 57 10.590 6.075 −1.624 1.00 17.57 C ATOM 190 C LYS A 57 10.291 5.833 −0.142 1.00 19.35 C ATOM 191 O LYS A 57 9.774 6.717 0.527 1.00 18.79 O ATOM 192 CB LYS A 57 12.025 6.586 −1.805 1.00 21.61 C ATOM 193 CG LYS A 57 13.121 5.642 −1.358 1.00 28.80 C ATOM 194 CD LYS A 57 14.479 6.303 −1.271 1.00 35.37 C ATOM 195 CE LYS A 57 15.444 5.400 −0.498 1.00 42.30 C ATOM 196 NZ LYS A 57 16.836 5.495 −1.024 1.00 47.58 N ATOM 197 N GLU A 58 10.553 4.624 0.331 1.00 19.15 N ATOM 198 CA GLU A 58 10.332 4.285 1.751 1.00 22.86 C ATOM 199 C GLU A 58 11.578 4.701 2.534 1.00 26.59 C ATOM 200 O GLU A 58 12.706 4.324 2.189 1.00 26.79 O ATOM 201 CB GLU A 58 10.036 2.778 1.890 1.00 28.00 C ATOM 202 CG GLU A 58 9.031 2.381 2.968 1.00 35.93 C ATOM 203 CD GLU A 58 8.290 1.076 2.629 1.00 41.37 C ATOM 204 OE1 GLU A 58 8.826 0.259 1.829 1.00 42.32 O ATOM 205 OE2 GLU A 58 7.165 0.857 3.162 1.00 46.12 O ATOM 206 N GLU A 59 11.343 5.505 3.563 1.00 25.97 N ATOM 207 CA GLU A 59 12.378 5.933 4.488 1.00 32.09 C ATOM 208 C GLU A 59 11.758 6.160 5.868 1.00 27.53 C ATOM 209 O GLU A 59 10.648 6.660 5.970 1.00 29.08 O ATOM 210 CB GLU A 59 13.017 7.227 3.995 1.00 34.91 C ATOM 211 CG GLU A 59 14.286 6.997 3.206 1.00 44.55 C ATOM 212 CD GLU A 59 14.719 8.235 2.460 1.00 49.72 C ATOM 213 OE1 GLU A 59 14.494 9.355 2.986 1.00 50.09 O ATOM 214 OE2 GLU A 59 15.284 8.082 1.352 1.00 59.01 O ATOM 215 N ASP A 60 12.504 5.808 6.919 1.00 30.78 N ATOM 216 CA ASP A 60 12.069 6.016 8.322 1.00 30.17 C ATOM 217 C ASP A 60 10.661 5.499 8.569 1.00 27.39 C ATOM 218 O ASP A 60 9.856 6.149 9.235 1.00 29.92 O ATOM 219 CB ASP A 60 12.142 7.509 8.738 1.00 36.59 C ATOM 220 CG ASP A 60 13.522 8.132 8.520 1.00 40.73 C ATOM 221 OD1 ASP A 60 14.569 7.471 8.742 1.00 43.76 O ATOM 222 OD2 ASP A 60 13.551 9.314 8.120 1.00 46.48 O ATOM 223 N GLY A 61 10.339 4.346 7.987 1.00 24.29 N ATOM 224 CA GLY A 61 9.047 3.725 8.169 1.00 24.48 C ATOM 225 C GLY A 61 7.852 4.187 7.337 1.00 26.72 C ATOM 226 O GLY A 61 6.725 3.741 7.554 1.00 27.47 O ATOM 227 N LYS A 62 8.079 5.085 6.383 1.00 24.80 N ATOM 228 CA LYS A 62 6.947 5.599 5.622 1.00 23.66 C ATOM 229 C LYS A 62 7.414 6.026 4.254 1.00 21.31 C ATOM 230 O LYS A 62 8.592 6.037 3.974 1.00 21.94 O ATOM 231 CB LYS A 62 6.310 6.766 6.359 1.00 29.91 C ATOM 232 CG LYS A 62 7.255 7.927 6.595 1.00 31.81 C ATOM 233 CD LYS A 62 6.563 8.952 7.496 1.00 38.79 C ATOM 234 CE LYS A 62 6.789 10.370 7.007 1.00 42.03 C ATOM 235 NZ LYS A 62 8.214 10.778 7.173 1.00 44.24 N ATOM 236 N PHE A 63 6.464 6.441 3.441 1.00 21.59 N ATOM 237 CA PHE A 63 6.808 6.954 2.106 1.00 20.61 C ATOM 238 C PHE A 63 6.998 8.448 2.109 1.00 20.37 C ATOM 239 O PHE A 63 6.191 9.175 2.714 1.00 24.87 O ATOM 240 CB PHE A 63 5.734 6.544 1.120 1.00 21.40 C ATOM 241 CG PHE A 63 5.871 5.133 0.694 1.00 21.27 C ATOM 242 CD1 PHE A 63 6.742 4.807 −0.324 1.00 20.89 C ATOM 243 CD2 PHE A 63 5.198 4.125 1.377 1.00 26.11 C ATOM 244 CE1 PHE A 63 6.866 3.501 −0.738 1.00 24.44 C ATOM 245 CE2 PHE A 63 5.343 2.799 0.985 1.00 26.70 C ATOM 246 CZ PHE A 63 6.184 2.496 −0.080 1.00 25.68 C ATOM 247 N VAL A 64 8.082 8.885 1.482 1.00 20.15 N ATOM 248 CA VAL A 64 8.386 10.292 1.241 1.00 20.78 C ATOM 249 C VAL A 64 8.691 10.523 −0.242 1.00 20.01 C ATOM 250 O VAL A 64 8.898 9.578 −1.015 1.00 17.55 O ATOM 251 CB VAL A 64 9.593 10.736 2.080 1.00 23.23 C ATOM 252 CG1 VAL A 64 9.357 10.443 3.573 1.00 26.08 C ATOM 253 CG2 VAL A 64 10.879 10.073 1.649 1.00 21.71 C ATOM 254 N GLU A 65 8.727 11.772 −0.679 1.00 19.26 N ATOM 255 CA GLU A 65 9.067 12.058 −2.078 1.00 18.64 C ATOM 256 C GLU A 65 10.504 11.705 −2.351 1.00 18.55 C ATOM 257 O GLU A 65 11.431 12.028 −1.593 1.00 20.32 O ATOM 258 CB GLU A 65 8.773 13.543 −2.430 1.00 20.30 C ATOM 259 CG GLU A 65 8.883 13.827 −3.929 1.00 20.56 C ATOM 260 CD GLU A 65 7.715 13.267 −4.757 1.00 21.82 C ATOM 261 OE1 GLU A 65 6.717 12.783 −4.227 1.00 24.78 O ATOM 262 OE2 GLU A 65 7.790 13.334 −6.018 1.00 21.81 O ATOM 263 N ALA A 66 10.777 11.007 −3.463 1.00 16.00 N ATOM 264 CA ALA A 66 12.100 10.688 −3.880 1.00 16.32 C ATOM 265 C ALA A 66 12.733 11.932 −4.503 1.00 16.97 C ATOM 266 O ALA A 66 12.013 12.671 −5.190 1.00 18.47 O ATOM 267 CB ALA A 66 12.076 9.547 −4.907 1.00 17.76 C ATOM 268 N ASN A 67 14.038 12.109 −4.369 1.00 17.37 N ATOM 269 CA ASN A 67 14.665 13.227 −5.057 1.00 17.30 C ATOM 270 C ASN A 67 15.037 12.853 −6.494 1.00 18.18 C ATOM 271 O ASN A 67 14.936 11.688 −6.910 1.00 16.63 O ATOM 272 CB ASN A 67 15.861 13.789 −4.252 1.00 18.42 C ATOM 273 CG ASN A 67 16.964 12.827 −4.098 1.00 20.58 C ATOM 274 OD1 ASN A 67 17.359 12.138 −5.015 1.00 18.66 O ATOM 275 ND2 ASN A 67 17.583 12.833 −2.889 1.00 25.30 N ATOM 276 N ASN A 67 15.507 13.813 −7.286 1.00 16.23 N ATOM 277 CA ASN A 67 15.843 13.561 −8.646 1.00 16.52 C ATOM 278 C ASN A 67 16.923 12.523 −8.828 1.00 16.04 C ATOM 279 O ASN A 67 16.854 11.699 −9.752 1.00 14.61 O ATOM 280 CB ASN A 67 16.246 14.879 −9.328 1.00 16.20 C ATOM 281 CG ASN A 68 15.083 15.746 −9.721 1.00 17.35 C ATOM 282 OD1 ASN A 68 15.301 16.922 −10.119 1.00 26.40 O ATOM 283 ND2 ASN A 68 13.885 15.262 −9.603 1.00 15.74 N ATOM 284 N ASN A 69 17.956 12.525 −7.974 1.00 17.17 N ATOM 285 CA ASN A 69 18.990 11.506 −8.109 1.00 18.83 C ATOM 286 C ASN A 69 18.434 10.102 −7.833 1.00 15.62 C ATOM 287 O ASN A 69 18.830 9.171 −8.527 1.00 16.15 O ATOM 288 CB ASN A 69 20.190 11.783 −7.165 1.00 22.39 C ATOM 289 CG ASN A 69 21.005 12.976 −7.624 1.00 30.98 C ATOM 290 OD1 ASN A 69 21.050 13.286 −8.812 1.00 35.68 O ATOM 291 ND2 ASN A 69 21.645 13.662 −6.689 1.00 35.75 N ATOM 292 N GLU A 70 17.564 9.980 −6.852 1.00 14.97 N ATOM 293 CA GLU A 70 17.000 8.685 −6.510 1.00 15.51 C ATOM 294 C GLU A 70 16.134 8.153 −7.656 1.00 14.44 C ATOM 295 O GLU A 70 16.153 6.990 −7.965 1.00 14.94 O ATOM 296 CB GLU A 70 16.188 8.773 −5.256 1.00 16.11 C ATOM 297 CG GLU A 70 17.111 8.948 −4.041 1.00 18.28 C ATOM 298 CD GLU A 70 16.378 9.405 −2.812 1.00 20.91 C ATOM 299 OE1 GLU A 70 15.312 10.033 −2.850 1.00 20.82 O ATOM 300 OE2 GLU A 70 16.932 9.149 −1.688 1.00 25.04 O ATOM 301 N ILE A 71 15.408 9.051 −8.288 1.00 14.15 N ATOM 302 CA ILE A 71 14.530 8.649 −9.436 1.00 12.01 C ATOM 303 C ILE A 71 15.405 8.163 −10.550 1.00 12.33 C ATOM 304 O ILE A 71 15.189 7.073 −11.083 1.00 11.90 O ATOM 305 CB ILE A 71 13.730 9.853 −9.925 1.00 12.43 C ATOM 306 CG1 ILE A 71 12.660 10.202 −8.953 1.00 12.90 C ATOM 307 CG2 ILE A 71 13.045 9.530 −11.280 1.00 11.69 C ATOM 308 CD1 ILE A 71 12.092 11.618 −9.135 1.00 14.32 C ATOM 309 N ALA A 72 16.453 8.903 −10.919 1.00 12.16 N ATOM 310 CA ALA A 72 17.354 8.512 −11.956 1.00 13.18 C ATOM 311 C ALA A 72 18.041 7.171 −11.684 1.00 13.53 C ATOM 312 O ALA A 72 18.161 6.331 −12.582 1.00 14.62 O ATOM 313 CB ALA A 72 18.423 9.609 −12.198 1.00 13.37 C ATOM 314 N LYS A 73 18.461 6.959 −10.419 1.00 14.30 N ATOM 315 CA LYS A 73 19.115 5.697 −10.107 1.00 17.04 C ATOM 316 C LYS A 73 18.136 4.546 −10.200 1.00 14.67 C ATOM 317 O LYS A 73 18.482 3.478 −10.709 1.00 14.89 O ATOM 318 CB LYS A 73 19.746 5.818 −8.709 1.00 19.83 C ATOM 319 CG LYS A 73 20.979 6.731 −8.704 1.00 26.39 C ATOM 320 CD LYS A 73 21.675 6.861 −7.345 1.00 33.08 C ATOM 321 CE LYS A 73 22.614 8.078 −7.383 1.00 37.13 C ATOM 322 NZ LYS A 73 23.427 8.322 −6.157 1.00 43.31 N ATOM 323 N ALA A 74 16.913 4.772 −9.759 1.00 13.64 N ATOM 324 CA ALA A 74 15.900 3.689 −9.744 1.00 12.63 C ATOM 325 C ALA A 74 15.535 3.197 −11.126 1.00 13.29 C ATOM 326 O ALA A 74 15.265 2.011 −11.346 1.00 14.88 O ATOM 327 CB ALA A 74 14.673 4.102 −8.964 1.00 13.11 C ATOM 328 N MET A 75 15.568 4.101 −12.102 1.00 12.75 N ATOM 329 CA MET A 75 15.191 3.734 −13.459 1.00 13.17 C ATOM 330 C MET A 75 16.352 3.231 −14.306 1.00 13.36 C ATOM 331 O MET A 75 16.173 2.764 −15.433 1.00 13.13 O ATOM 332 CB MET A 75 14.386 4.910 −14.093 1.00 16.87 C ATOM 333 CG MET A 75 15.116 6.148 −14.238 1.00 16.12 C ATOM 334 SD MET A 75 14.057 7.538 −14.804 1.00 11.79 S ATOM 335 CE MET A 75 13.921 7.112 −16.529 1.00 11.78 C ATOM 336 N THR A 76 17.591 3.255 −13.776 1.00 14.14 N ATOM 337 CA THR A 76 18.710 2.700 −14.476 1.00 15.83 C ATOM 338 C THR A 76 18.573 1.191 −14.653 1.00 16.94 C ATOM 339 O THR A 76 18.051 0.512 −13.787 1.00 18.64 O ATOM 340 CB THR A 76 20.001 3.057 −13.688 1.00 18.74 C ATOM 341 OG1 THR A 76 20.122 4.478 −13.650 1.00 21.20 O ATOM 342 CG2 THR A 76 21.216 2.536 −14.325 1.00 20.55 C ATOM 343 N ILE A 77 19.016 0.675 −15.786 1.00 18.90 N ATOM 344 CA ILE A 77 18.871 −0.737 −16.060 1.00 21.35 C ATOM 345 C ILE A 77 20.085 −1.534 −15.615 1.00 26.22 C ATOM 346 O ILE A 77 21.190 −1.257 −16.059 1.00 25.60 O ATOM 347 CB ILE A 77 18.548 −0.978 −17.512 1.00 23.15 C ATOM 348 CG1 ILE A 77 17.098 −0.484 −17.758 1.00 25.49 C ATOM 349 CG2 ILE A 77 18.629 −2.464 −17.853 1.00 22.44 C ATOM 350 CD1 ILE A 77 16.949 0.109 −19.095 1.00 27.57 C ATOM 351 N SER A 78 19.839 −2.514 −14.755 1.00 28.05 N ATOM 352 CA SER A 78 20.919 −3.271 −14.086 1.00 35.77 C ATOM 353 C SER A 78 20.566 −4.743 −13.934 1.00 38.52 C ATOM 354 O SER A 78 20.020 −5.337 −14.867 1.00 46.11 O ATOM 355 CB SER A 78 21.193 −2.690 −12.707 1.00 39.22 C ATOM 356 OG SER A 78 21.436 −1.299 −12.791 1.00 49.48 O ATOM 357 N ASP A 81 17.749 −9.240 −9.122 1.00 29.91 N ATOM 358 CA ASP A 81 16.763 −10.324 −8.942 1.00 30.18 C ATOM 359 C ASP A 81 16.650 −11.158 −10.213 1.00 29.37 C ATOM 360 O ASP A 81 15.518 −11.413 −10.630 1.00 27.18 O ATOM 361 CB ASP A 81 15.327 −9.792 −8.535 1.00 29.40 C ATOM 362 CG ASP A 81 15.304 −9.039 −7.174 1.00 33.79 C ATOM 363 OD1 ASP A 81 15.894 −9.591 −6.206 1.00 35.75 O ATOM 364 OD2 ASP A 81 14.684 −7.923 −7.027 1.00 33.56 O ATOM 365 N ASN A 82 17.791 −11.614 −10.783 1.00 28.93 N ATOM 366 CA ASN A 82 17.828 −12.503 −11.978 1.00 24.97 C ATOM 367 C ASN A 82 16.993 −11.910 −13.138 1.00 23.71 C ATOM 368 O ASN A 82 16.385 −12.638 −13.990 1.00 23.74 O ATOM 369 CB ASN A 82 17.347 −13.919 −11.637 1.00 27.93 C ATOM 370 CG ASN A 82 18.345 −14.664 −10.778 1.00 29.75 C ATOM 371 OD1 ASN A 82 19.241 −15.375 −11.282 1.00 35.66 O ATOM 372 ND2 ASN A 82 18.234 −14.472 −9.487 1.00 26.95 N ATOM 373 N ASP A 83 16.976 −10.565 −13.154 1.00 20.97 N ATOM 374 CA ASP A 83 16.309 −9.854 −14.235 1.00 22.23 C ATOM 375 C ASP A 83 14.819 −10.120 −14.246 1.00 18.07 C ATOM 376 O ASP A 83 14.183 −9.958 −15.312 1.00 14.19 O ATOM 377 CB ASP A 83 16.876 −10.186 −15.620 1.00 27.65 C ATOM 378 CG ASP A 83 18.338 −9.769 −15.837 1.00 31.98 C ATOM 379 OD1 ASP A 83 18.754 −8.620 −15.601 1.00 36.88 O ATOM 380 OD2 ASP A 83 19.055 −10.636 −16.372 1.00 38.49 O ATOM 381 N MET A 84 14.204 −10.488 −13.128 1.00 13.74 N ATOM 382 CA MET A 84 12.766 −10.700 −13.081 1.00 14.26 C ATOM 383 C MET A 84 12.023 −9.406 −13.433 1.00 11.96 C ATOM 384 O MET A 84 10.922 −9.496 −13.981 1.00 11.99 O ATOM 385 CB MET A 84 12.188 −11.139 −11.698 1.00 15.85 C ATOM 386 CG MET A 84 12.336 −12.519 −11.129 1.00 20.01 C ATOM 387 SD MET A 84 11.258 −13.670 −12.051 1.00 26.25 S ATOM 388 CE MET A 84 12.651 −14.093 −12.949 1.00 16.45 C ATOM 389 N LYS A 85 12.563 −8.217 −13.149 1.00 10.40 N ATOM 390 CA ALYS A 85 11.938 −6.925 −13.531 0.70 10.95 C ATOM 391 CA BLYS A 85 11.788 −7.030 −13.520 0.30 10.01 C ATOM 392 C LYS A 85 11.826 −6.793 −15.024 1.00 10.17 C ATOM 393 O LYS A 85 11.038 −5.946 −15.512 1.00 9.05 O ATOM 394 CB ALYS A 85 12.793 −5.753 −13.020 0.70 12.73 C ATOM 395 CB BLYS A 85 12.211 −5.813 −12.724 0.30 10.03 C ATOM 396 CG ALYS A 85 12.853 −5.618 −11.507 0.70 16.14 C ATOM 397 CG BLYS A 85 13.595 −5.311 −13.067 0.30 10.33 C ATOM 398 CF ALYS A 85 13.360 −4.238 −11.077 0.70 16.2 C ATOM 399 CF BLYS A 85 13.880 −4.016 −12.331 0.30 10.73 C ATOM 400 CE ALYS A 85 14.792 −3.899 −11.515 0.70 18.08 C ATOM 401 CE BLYS A 85 14.470 −4.207 −10.940 0.30 11.00 C ATOM 402 NZ ALYS A 85 15.169 −2.629 −10.807 0.70 17.19 N ATOM 403 NZ BLYS A 85 14.951 −2.880 −10.414 0.30 10.70 N ATOM 404 N TYR A 86 12.680 −7.492 −15.768 1.00 8.67 N ATOM 405 CA TYR A 86 12.759 −7.359 −17.210 1.00 9.18 C ATOM 406 C TYR A 86 12.191 −8.526 −17.961 1.00 9.12 C ATOM 407 O TYR A 86 12.320 −8.626 −19.186 1.00 9.75 O ATOM 408 CB TYR A 86 14.253 −7.126 −17.586 1.00 9.54 C ATOM 409 CG TYR A 86 14.895 −6.003 −16.844 1.00 10.25 C ATOM 410 CD1 TYR A 86 14.522 −4.704 −17.062 1.00 11.51 C ATOM 411 CD2 TYR A 86 15.842 −6.228 −15.867 1.00 11.51 C ATOM 412 CE1 TYR A 86 15.040 −3.677 −16.352 1.00 12.06 C ATOM 413 CE2 TYR A 86 16.413 −5.195 −15.166 1.00 13.01 C ATOM 414 CZ TYR A 86 16.020 −3.917 −15.415 1.00 13.13 C ATOM 415 OH TYR A 86 16.606 −2.902 −14.659 1.00 15.89 O ATOM 416 N MET A 87 11.514 −9.439 −17.252 1.00 8.35 N ATOM 417 CA MET A 87 10.998 −10.647 −17.824 1.00 8.34 C ATOM 418 C MET A 87 9.544 −10.509 −18.245 1.00 8.28 C ATOM 419 O MET A 87 8.681 −10.033 −17.476 1.00 8.19 O ATOM 420 CB MET A 87 11.086 −11.802 −16.791 1.00 8.79 C ATOM 421 CG MET A 87 10.741 −13.145 −17.384 1.00 8.66 C ATOM 422 SD MET A 87 10.516 −14.431 −16.127 1.00 9.82 S ATOM 423 CE MET A 87 8.974 −13.860 −15.454 1.00 10.45 C ATOM 424 N ASP A 88 9.208 −10.968 −19.434 1.00 8.46 N ATOM 425 CA ASP A 88 7.842 −10.959 −19.919 1.00 8.62 C ATOM 426 C ASP A 88 6.945 −11.720 −18.951 1.00 8.30 C ATOM 427 O ASP A 88 7.227 −12.920 −18.677 1.00 8.85 O ATOM 428 CB ASP A 88 7.866 −11.654 −21.301 1.00 9.83 C ATOM 429 CG ASP A 88 6.577 −11.697 −21.985 1.00 11.82 C ATOM 430 OD1 ASP A 88 5.503 −11.347 −21.486 1.00 10.23 O ATOM 431 OD2 ASP A 88 6.617 −12.171 −23.165 1.00 17.68 O ATOM 432 N ILE A 89 5.895 −11.068 −18.450 1.00 7.20 N ATOM 433 CA ILE A 89 4.945 −11.682 −17.505 1.00 7.68 C ATOM 434 C ILE A 89 3.600 −11.894 −18.154 1.00 7.54 C ATOM 435 O ILE A 89 2.577 −12.121 −17.458 1.00 7.42 O ATOM 436 CB ILE A 89 4.850 −10.846 −16.200 1.00 7.37 C ATOM 437 CG1 ILE A 89 4.711 −9.325 −16.485 1.00 7.30 C ATOM 438 CG2 ILE A 89 6.036 −11.160 −15.298 1.00 7.41 C ATOM 439 CD1 ILE A 89 4.392 −8.462 −15.267 1.00 7.30 C ATOM 440 N THR A 90 3.572 −11.856 −19.499 1.00 7.80 N ATOM 441 CA THR A 90 2.348 −12.014 −20.262 1.00 8.47 C ATOM 442 C THR A 90 2.205 −13.424 −20.766 1.00 9.89 C ATOM 443 O THR A 90 1.355 −13.627 −21.644 1.00 10.30 O ATOM 444 CB THR A 90 2.288 −10.953 −21.439 1.00 9.39 C ATOM 445 OG1 THR A 90 3.112 −11.412 −22.513 1.00 11.38 O ATOM 446 CG2 THR A 90 2.655 −9.625 −20.981 1.00 10.26 C ATOM 447 N GLU A 91 2.951 −14.355 −20.227 1.00 10.10 N ATOM 448 CA AGLU A 91 2.834 −15.788 −20.545 0.50 12.01 C ATOM 449 CA BGLU A 91 2.747 −15.764 −20.545 0.50 12.03 C ATOM 450 C GLU A 91 2.449 −16.503 −19.263 1.00 12.99 C ATOM 451 O GLU A 91 2.477 −15.938 −18.196 1.00 13.57 O ATOM 452 CB AGLU A 91 4.170 −16.351 −21.070 0.50 13.47 C ATOM 453 CB BGLU A 91 3.972 −16.299 −21.266 0.50 13.97 C ATOM 454 CG AGLU A 91 4.774 −15.645 −22.288 0.50 15.79 C ATOM 455 CG BGLU A 91 4.159 −15.626 −22.625 0.50 16.36 C ATOM 456 CD AGLU A 91 4.091 −15.985 −23.607 0.50 18.48 C ATOM 457 CD BGLU A 91 5.047 −16.424 −23.536 0.50 19.06 C ATOM 458 OE1 AGLU A 91 2.978 −16.511 −23.624 0.50 22.02 O ATOM 459 OE1 BGLU A 91 6.217 −16.598 −23.174 0.50 21.30 O ATOM 460 OE2 AGLU A 91 4.707 −15.731 −24.665 0.50 23.00 O ATOM 461 OE2 BGLU A 91 4.546 −16.875 −24.609 0.50 24.10 O ATOM 462 N LYS A 92 2.069 −17.730 −19.385 1.00 11.71 N ATOM 463 CA LYS A 92 1.605 −18.552 −18.266 1.00 11.28 C ATOM 464 C LYS A 92 2.707 −19.402 −17.766 1.00 11.53 C ATOM 465 O LYS A 92 3.672 −19.673 −18.513 1.00 12.63 O ATOM 466 CB LYS A 92 0.447 −19.371 −18.726 1.00 12.06 C ATOM 467 CG LYS A 92 −0.682 −18.486 −19.159 1.00 12.14 C ATOM 468 CD LYS A 92 −1.908 −19.241 −19.509 1.00 13.26 C ATOM 469 CE LYS A 92 −1.694 −19.998 −20.799 1.00 16.69 C ATOM 470 NZ LYS A 92 −2.969 −20.498 −21.223 1.00 18.41 N ATOM 471 N VAL A 93 2.650 −19.819 −16.503 1.00 9.15 N ATOM 472 CA VAL A 93 3.630 −20.728 −15.931 1.00 9.90 C ATOM 473 C VAL A 93 2.942 −21.973 −15.440 1.00 9.37 C ATOM 474 O VAL A 93 1.785 −21.937 −15.043 1.00 9.19 O ATOM 475 CB VAL A 93 4.441 −20.073 −14.791 1.00 9.88 C ATOM 476 CG1 VAL A 93 5.396 −19.034 −15.369 1.00 11.74 C ATOM 477 CG2 VAL A 93 3.513 −19.549 −13.721 1.00 10.87 C ATOM 478 N PRO A 94 3.692 −23.079 −15.426 1.00 10.41 N ATOM 479 CA PRO A 94 3.118 −24.349 −15.030 1.00 10.62 C ATOM 480 C PRO A 94 3.130 −24.462 −13.524 1.00 11.29 C ATOM 481 O PRO A 94 4.089 −24.907 −12.890 1.00 15.61 O ATOM 482 CB PRO A 94 4.101 −25.382 −15.662 1.00 11.06 C ATOM 483 CG PRO A 94 5.417 −24.673 −15.722 1.00 12.08 C ATOM 484 CD PRO A 94 5.049 −23.220 −15.982 1.00 11.41 C ATOM 485 N MET A 95 2.074 −23.965 −12.913 1.00 8.87 N ATOM 486 CA MET A 95 1.862 −24.033 −11.484 1.00 8.96 C ATOM 487 C MET A 95 0.498 −24.628 −11.215 1.00 8.49 C ATOM 488 O MET A 95 −0.514 −24.054 −11.632 1.00 9.06 O ATOM 489 CB MET A 95 1.920 −22.636 −10.854 1.00 8.80 C ATOM 490 CG MET A 95 3.340 −22.095 −10.756 1.00 9.53 C ATOM 491 SD MET A 95 4.378 −22.750 −9.457 1.00 10.80 S ATOM 492 CE MET A 95 3.689 −21.895 −8.070 1.00 11.28 C ATOM 493 N SER A 96 0.455 −25.722 −10.487 1.00 7.98 N ATOM 494 CA SER A 96 −0.844 −26.323 −10.123 1.00 9.02 C ATOM 495 C SER A 96 −1.545 −25.554 −9.031 1.00 8.82 C ATOM 496 O SER A 96 −0.906 −24.722 −8.351 1.00 7.77 O ATOM 497 CB SER A 96 −0.630 −27.731 −9.671 1.00 9.38 C ATOM 498 OG SER A 96 0.071 −27.713 −8.447 1.00 9.85 O ATOM 499 N GLU A 97 −2.818 −25.823 −8.800 1.00 8.52 N ATOM 500 CA GLU A 97 −3.518 −25.122 −7.726 1.00 10.00 C ATOM 501 C GLU A 97 −2.878 −25.472 −6.388 1.00 10.09 C ATOM 502 O GLU A 97 −2.805 −24.605 −5.519 1.00 9.76 O ATOM 503 CB GLU A 97 −5.001 −25.401 −7.781 1.00 12.99 C ATOM 504 CG GLU A 97 −5.413 −26.777 −7.474 1.00 17.01 C ATOM 505 CD GLU A 97 −5.674 −27.055 −5.992 1.00 21.45 C ATOM 506 OE1 GLU A 97 −5.766 −28.258 −5.609 1.00 23.75 O ATOM 507 OE2 GLU A 97 −5.781 −26.081 −5.208 1.00 25.48 O ATOM 508 N SER A 98 −2.337 −26.664 −6.222 1.00 9.61 N ATOM 509 CA ASER A 98 −1.650 −27.049 −4.992 0.50 9.84 C ATOM 510 CA BSER A 98 −1.726 −26.967 −4.946 0.50 10.33 C ATOM 511 C SER A 98 −0.425 −26.171 −4.795 1.00 9.61 C ATOM 512 O SER A 98 −0.189 −25.651 −3.726 1.00 9.80 O ATOM 513 CB ASER A 98 −1.185 −28.475 −5.103 0.50 10.59 C ATOM 514 CB BSER A 98 −1.536 −28.450 −4.771 0.50 11.67 C ATOM 515 OG ASER A 98 −2.263 −29.390 −4.986 0.50 11.69 O ATOM 516 OG BSER A 98 −0.595 −28.973 −5.693 0.50 14.49 O ATOM 517 N GLU A 99 0.356 −25.983 −5.864 1.00 8.51 N ATOM 518 CA GLU A 99 1.548 −25.139 −5.768 1.00 8.92 C ATOM 519 C GLU A 99 1.217 −23.695 −5.487 1.00 8.55 C ATOM 520 O GLU A 99 1.881 −23.036 −4.641 1.00 8.89 O ATOM 521 CB GLU A 99 2.360 −25.242 −7.036 1.00 9.61 C ATOM 522 CG GLU A 99 3.020 −26.622 −7.207 1.00 11.26 C ATOM 523 CD GLU A 99 3.628 −26.708 −8.567 1.00 12.96 C ATOM 524 OE1 GLU A 99 3.018 −26.617 −9.631 1.00 11.71 O ATOM 525 OE2 GLU A 99 4.906 −26.928 −8.624 1.00 22.38 O ATOM 526 N VAL A 100 0.203 −23.158 −6.143 1.00 7.88 N ATOM 527 CA VAL A 100 −0.192 −21.769 −5.866 1.00 7.97 C ATOM 528 C VAL A 100 −0.675 −21.630 −4.423 1.00 8.83 C ATOM 529 O VAL A 100 −0.300 −20.659 −3.743 1.00 9.14 O ATOM 530 CB VAL A 100 −1.219 −21.280 −6.902 1.00 8.30 C ATOM 531 CG1 VAL A 100 −1.619 −19.836 −6.582 1.00 8.66 C ATOM 532 CG2 VAL A 100 −0.610 −21.363 −8.310 1.00 7.94 C ATOM 533 N ASN A 101 −1.453 −22.573 −3.948 1.00 8.38 N ATOM 534 CA ASN A 101 −1.932 −22.514 −2.538 1.00 8.64 C ATOM 535 C ASN A 101 −0.793 −22.636 −1.567 1.00 9.24 C ATOM 536 O ASN A 101 −0.804 −22.022 −0.499 1.00 10.73 O ATOM 537 CB ASN A 101 −3.014 −23.532 −2.279 1.00 8.69 C ATOM 538 CG ASN A 101 −4.346 −23.016 −2.725 1.00 9.04 C ATOM 539 OD1 ASN A 101 −4.624 −21.843 −2.574 1.00 9.61 O ATOM 540 ND2 ASN A 101 −5.194 −23.881 −3.229 1.00 11.02 N ATOM 541 N GLN A 102 0.260 −23.337 −1.954 1.00 9.84 N ATOM 542 CA GLN A 102 1.468 −23.378 −1.092 1.00 10.88 C ATOM 543 C GLN A 102 2.028 −21.984 −0.934 1.00 11.90 C ATOM 544 O GLN A 102 2.411 −21.554 0.195 1.00 13.07 O ATOM 545 CB GLN A 102 2.548 −24.373 −1.636 1.00 11.93 C ATOM 546 CG GLN A 102 2.176 −25.833 −1.484 1.00 13.56 C ATOM 547 CD GLN A 102 2.712 −26.820 −2.553 1.00 16.52 C ATOM 548 OE1 GLN A 102 3.758 −26.605 −3.103 1.00 13.95 O ATOM 549 NE2 GLN A 102 2.000 −27.933 −2.802 1.00 19.51 N ATOM 550 N LEU A 103 2.104 −21.191 −2.012 1.00 10.81 N ATOM 551 CA LEU A 103 2.559 −19.796 −1.930 1.00 11.86 C ATOM 552 C LEU A 103 1.664 −18.937 −1.094 1.00 12.35 C ATOM 553 O LEU A 103 2.117 −17.964 −0.561 1.00 13.65 O ATOM 554 CB LEU A 103 2.722 −19.142 −3.287 1.00 13.39 C ATOM 555 CG LEU A 103 3.845 −19.683 −4.158 1.00 14.81 C ATOM 556 CD1 LEU A 103 3.777 −18.956 −5.449 1.00 15.22 C ATOM 557 CD2 LEU A 103 5.178 −19.490 −3.504 1.00 15.04 C ATOM 558 N LEU A 104 0.364 −19.241 −1.055 1.00 10.26 N ATOM 559 CA LEU A 104 −0.614 −18.414 −0.350 1.00 9.22 C ATOM 560 C LEU A 104 −0.876 −18.859 1.062 1.00 9.62 C ATOM 561 O LEU A 104 −1.625 −18.212 1.744 1.00 9.83 O ATOM 562 CB LEU A 104 −1.910 −18.419 −1.148 1.00 8.86 C ATOM 563 CG LEU A 104 −1.835 −17.781 −2.559 1.00 8.53 C ATOM 564 CD1 LEU A 104 −3.151 −18.028 −3.285 1.00 8.70 C ATOM 565 CD2 LEU A 104 −1.486 −16.350 −2.488 1.00 9.69 C ATOM 566 N LYS A 105 −0.258 −19.939 1.484 1.00 10.21 N ATOM 567 CA LYS A 105 −0.444 −20.431 2.852 1.00 11.03 C ATOM 568 C LYS A 105 0.072 −19.355 3.829 1.00 11.31 C ATOM 569 O LYS A 105 1.162 −18.784 3.665 1.00 12.43 O ATOM 570 CB LYS A 105 0.294 −21.754 3.085 1.00 13.39 C ATOM 571 CG LYS A 105 −0.046 −22.456 4.395 1.00 17.18 C ATOM 572 CD LYS A 105 −1.489 −22.984 4.272 1.00 22.14 C ATOM 573 CE LYS A 105 −2.015 −23.682 5.509 1.00 29.14 C ATOM 574 NZ LYS A 105 −3.423 −24.077 5.190 1.00 33.24 N ATOM 575 N GLY A 106 −0.756 −19.043 4.829 1.00 11.10 N ATOM 576 CA GLY A 106 −0.400 −17.991 5.752 1.00 11.09 C ATOM 577 C GLY A 106 −0.491 −16.576 5.228 1.00 10.78 C ATOM 578 O GLY A 106 0.011 −15.614 5.871 1.00 13.32 O ATOM 579 N LYS A 107 −1.220 −16.347 4.120 1.00 10.32 N ATOM 580 CA LYS A 107 −1.312 −15.044 3.511 1.00 10.14 C ATOM 581 C LYS A 107 −2.719 −14.440 3.626 1.00 9.55 C ATOM 582 O LYS A 107 −3.294 −13.884 2.706 1.00 8.67 O ATOM 583 CB LYS A 107 −0.874 −15.107 2.020 1.00 10.51 C ATOM 584 CG LYS A 107 0.510 −15.683 1.780 1.00 11.01 C ATOM 585 CD LYS A 107 1.607 −15.001 2.584 1.00 13.60 C ATOM 586 CE LYS A 107 2.976 −15.476 2.139 1.00 16.14 C ATOM 587 NZ LYS A 107 4.027 −14.752 2.917 1.00 20.86 N ATOM 588 N GLY A 108 −3.247 −14.513 4.845 1.00 8.91 N ATOM 589 CA GLY A 108 −4.416 −13.766 5.172 1.00 8.32 C ATOM 590 C GLY A 108 −5.644 −14.033 4.289 1.00 7.86 C ATOM 591 O GLY A 108 −5.999 −15.180 4.073 1.00 7.44 O ATOM 592 N ILE A 109 −6.227 −12.974 3.777 1.00 7.60 N ATOM 593 CA ILE A 109 −7.388 −13.052 2.884 1.00 8.09 C ATOM 594 C ILE A 109 −7.077 −13.772 1.571 1.00 7.60 C ATOM 595 O ILE A 109 −8.021 −14.195 0.904 1.00 8.06 O ATOM 596 CB ILE A 109 −7.999 −11.676 2.664 1.00 7.96 C ATOM 597 CG1 ILE A 109 −9.406 −11.750 2.125 1.00 7.94 C ATOM 598 CG2 ILE A 109 −7.110 −10.819 1.791 1.00 8.35 C ATOM 599 CD1 ILE A 109 −10.148 −10.449 2.322 1.00 7.40 C ATOM 600 N LEU A 110 −5.808 −13.913 1.227 1.00 7.86 N ATOM 601 CA LEU A 110 −5.457 −14.606 −0.001 1.00 7.42 C ATOM 602 C LEU A 110 −5.239 −16.085 0.169 1.00 7.66 C ATOM 603 O LEU A 110 −5.171 −16.818 −0.833 1.00 7.83 O ATOM 604 CB LEU A 110 −4.224 −13.945 −0.622 1.00 8.05 C ATOM 605 CG LEU A 110 −4.355 −12.480 −0.926 1.00 8.95 C ATOM 606 CD1 LEU A 110 −3.011 −11.941 −1.416 1.00 11.01 C ATOM 607 CD2 LEU A 110 −5.483 −12.166 −1.904 1.00 9.35 C ATOM 608 N GLU A 111 −5.105 −16.588 1.409 1.00 7.45 N ATOM 609 CA GLU A 111 −4.900 −17.972 1.661 1.00 7.85 C ATOM 610 C GLU A 111 −6.024 −18.800 1.035 1.00 7.85 C ATOM 611 O GLU A 111 −7.215 −18.468 1.180 1.00 7.89 O ATOM 612 CB GLU A 111 −4.775 −18.244 3.192 1.00 8.78 C ATOM 613 CG GLU A 111 −4.546 −19.689 3.528 1.00 9.27 C ATOM 614 CD GLU A 111 −4.372 −19.839 5.026 1.00 11.58 C ATOM 615 OE1 GLU A 111 −5.413 −19.773 5.727 1.00 12.28 O ATOM 616 OE2 GLU A 111 −3.218 −19.954 5.498 1.00 12.02 O ATOM 617 N ASN A 112 −5.657 −19.921 0.428 1.00 8.12 N ATOM 618 CA ASN A 112 −6.577 −20.845 −0.139 1.00 9.56 C ATOM 619 C ASN A 112 −7.434 −20.262 −1.232 1.00 8.71 C ATOM 620 O ASN A 112 −8.547 −20.704 −1.487 1.00 10.62 O ATOM 621 CB ASN A 112 −7.394 −21.574 0.951 1.00 10.76 C ATOM 622 CG ASN A 112 −6.628 −22.712 1.597 1.00 14.64 C ATOM 623 OD1 ASN A 112 −6.866 −23.045 2.762 1.00 19.98 O ATOM 624 ND2 ASN A 112 −5.661 −23.263 0.890 1.00 16.46 N ATOM 625 N ARG A 113 −6.887 −19.289 −1.975 1.00 7.64 N ATOM 626 CA ARG A 113 −7.526 −18.722 −3.145 1.00 7.57 C ATOM 627 C ARG A 113 −6.740 −19.057 −4.432 1.00 6.96 C ATOM 628 O ARG A 113 −6.941 −18.423 −5.483 1.00 7.19 O ATOM 629 CB ARG A 113 −7.823 −17.209 −3.034 1.00 7.60 C ATOM 630 CG ARG A 113 −8.720 −16.949 −1.825 1.00 7.92 C ATOM 631 CD ARG A 113 −9.273 −15.557 −1.849 1.00 8.34 C ATOM 632 NE ARG A 113 −10.046 −15.316 −0.616 1.00 9.26 N ATOM 633 CZ ARG A 113 −11.321 −15.618 −0.416 1.00 9.69 C ATOM 634 NH1 ARG A 113 −12.078 −16.071 −1.386 1.00 9.99 N ATOM 635 NH2 ARG A 113 −11.849 −15.412 0.761 1.00 10.68 N ATOM 636 N GLY A 114 −5.878 −20.066 −4.349 1.00 6.95 N ATOM 637 CA GLY A 114 −5.065 −20.443 −5.504 1.00 7.17 C ATOM 638 C GLY A 114 −5.882 −20.812 −6.727 1.00 7.63 C ATOM 639 O GLY A 114 −5.448 −20.584 −7.881 1.00 8.50 O ATOM 640 N LYS A 115 −6.995 −21.504 −6.527 1.00 7.70 N ATOM 641 CA ALYS A 115 −7.773 −21.904 −7.700 0.60 8.59 C ATOM 642 CA BLYS A 115 −7.907 −21.883 −7.616 0.40 8.13 C ATOM 643 C LYS A 115 −8.221 −20.691 −8.496 1.00 8.25 C ATOM 644 O LYS A 115 −8.293 −20.749 −9.750 1.00 8.11 O ATOM 645 CB ALYS A 115 −8.971 −22.809 −7.397 0.60 10.46 C ATOM 646 CB BLYS A 115 −9.221 −22.417 −7.021 0.40 9.12 C ATOM 647 CG ALYS A 115 −8.617 −24.183 −6.882 0.60 12.46 C ATOM 648 CG BLYS A 115 −10.405 −22.435 −7.945 0.40 9.60 C ATOM 649 CD ALYS A 115 −9.957 −24.761 −6.473 0.60 15.40 C ATOM 650 CD BLYS A 115 −11.623 −22.968 −7.284 0.40 10.43 C ATOM 651 CE ALYS A 115 −9.937 −26.006 −5.714 0.60 17.77 C ATOM 652 CE BLYS A 115 −12.752 −23.120 −8.270 0.40 10.49 C ATOM 653 NZ ALYS A 115 −11.342 −26.174 −5.391 0.60 20.66 N ATOM 654 NZ BLYS A 115 −13.886 −23.819 −7.676 0.40 10.66 N ATOM 655 N VAL A 116 −8.515 −19.579 −7.820 1.00 7.79 N ATOM 656 CA VAL A 116 −8.984 −18.369 −8.484 1.00 8.11 C ATOM 657 C VAL A 116 −7.835 −17.608 −9.161 1.00 7.43 C ATOM 658 O VAL A 116 −7.959 −17.079 −10.264 1.00 7.87 O ATOM 659 CB VAL A 116 −9.786 −17.505 −7.502 1.00 9.94 C ATOM 660 CG1 VAL A 116 −10.250 −16.261 −8.190 1.00 10.14 C ATOM 661 CG2 VAL A 116 −11.015 −18.284 −7.015 1.00 11.71 C ATOM 662 N PHE A 117 −6.674 −17.558 −8.504 1.00 6.80 N ATOM 663 CA PHE A 117 −5.502 −17.020 −9.203 1.00 6.68 C ATOM 664 C PHE A 117 −5.241 −17.783 −10.526 1.00 6.57 C ATOM 665 O PHE A 117 −4.836 −17.179 −11.506 1.00 6.36 O ATOM 666 CB PHE A 117 −4.292 −17.066 −8.282 1.00 7.12 C ATOM 667 CG PHE A 117 −4.183 −15.901 −7.309 1.00 7.07 C ATOM 668 CD1 PHE A 117 −3.566 −14.722 −7.705 1.00 7.49 C ATOM 669 CD2 PHE A 117 −4.745 −15.934 −6.039 1.00 7.19 C ATOM 670 CE1 PHE A 117 −3.443 −13.614 −6.831 1.00 7.29 C ATOM 671 CE2 PHE A 117 −4.586 −14.847 −5.164 1.00 7.84 C ATOM 672 CZ PHE A 117 −3.981 −13.687 −5.578 1.00 8.01 C ATOM 673 N LEU A 118 −5.407 −19.113 −10.515 1.00 7.10 N ATOM 674 CA LEU A 118 −5.189 −19.853 −11.751 1.00 7.12 C ATOM 675 C LEU A 118 −6.339 −19.644 −12.760 1.00 6.69 C ATOM 676 O LEU A 118 −6.032 −19.615 −13.968 1.00 6.72 O ATOM 677 CB LEU A 118 −5.001 −21.339 −11.445 1.00 7.31 C ATOM 678 CG LEU A 118 −3.604 −21.641 −10.910 1.00 8.29 C ATOM 679 CD1 LEU A 118 −3.576 −23.054 −10.361 1.00 9.66 C ATOM 680 CD2 LEU A 118 −2.506 −21.382 −11.925 1.00 8.59 C ATOM 681 N GLU A 119 −7.581 −19.493 −12.332 1.00 6.87 N ATOM 682 CA GLU A 119 −8.623 −19.042 −13.245 1.00 7.96 C ATOM 683 C GLU A 119 −8.196 −17.768 −13.957 1.00 7.79 C ATOM 684 O GLU A 119 −8.333 −17.695 −15.190 1.00 7.93 O ATOM 685 CB GLU A 119 −9.940 −18.821 −12.548 1.00 10.12 C ATOM 686 CG GLU A 119 −10.593 −20.058 −11.990 1.00 13.93 C ATOM 687 CD GLU A 119 −11.785 −19.729 −11.074 1.00 19.54 C ATOM 688 OE1 GLU A 119 −12.155 −18.544 −10.922 1.00 23.43 O ATOM 689 OE2 GLU A 119 −12.357 −20.674 −10.486 1.00 26.48 O ATOM 690 N ALA A 120 −7.677 −16.803 −13.192 1.00 7.92 N ATOM 691 CA ALA A 120 −7.199 −15.568 −13.779 1.00 7.95 C ATOM 692 C ALA A 120 −6.064 −15.785 −14.764 1.00 7.26 C ATOM 693 O ALA A 120 −6.038 −15.216 −15.870 1.00 7.30 O ATOM 694 CB ALA A 120 −6.775 −14.648 −12.652 1.00 8.18 C ATOM 695 N GLN A 121 −5.083 −16.611 −14.385 1.00 6.76 N ATOM 696 CA GLN A 121 −3.974 −16.883 −15.304 1.00 6.72 C ATOM 697 C GLN A 121 −4.487 −17.392 −16.652 1.00 7.64 C ATOM 698 O GLN A 121 −4.025 −16.938 −17.711 1.00 7.78 O ATOM 699 CB GLN A 121 −2.962 −17.894 −14.691 1.00 6.78 C ATOM 700 CG GLN A 121 −1.973 −18.359 −15.745 1.00 6.98 C ATOM 701 CD GLN A 121 −0.775 −19.123 −15.256 1.00 6.64 C ATOM 702 OE1 GLN A 121 0.323 −18.619 −15.043 1.00 6.57 O ATOM 703 NE2 GLN A 121 −0.971 −20.436 −15.122 1.00 8.15 N ATOM 704 N GLU A 122 −5.382 −18.387 −16.607 1.00 7.84 N ATOM 705 CA GLU A 122 −5.792 −19.021 −17.845 1.00 8.16 C ATOM 706 C GLU A 122 −6.754 −18.128 −18.622 1.00 8.14 C ATOM 707 O GLU A 122 −6.650 −18.062 −19.853 1.00 8.85 O ATOM 708 CB GLU A 122 −6.430 −20.385 −17.608 1.00 9.37 C ATOM 709 CG GLU A 122 −5.520 −21.374 −16.837 1.00 10.77 C ATOM 710 CD GLU A 122 −4.212 −21.601 −17.526 1.00 14.44 C ATOM 711 OE1 GLU A 122 −4.231 −21.727 −18.751 1.00 18.07 O ATOM 712 OE2 GLU A 122 −3.155 −21.670 −16.878 1.00 16.64 O ATOM 713 N LYS A 123 −7.672 −17.455 −17.965 1.00 8.18 N ATOM 714 CA LYS A 123 −8.626 −16.617 −18.680 1.00 8.51 C ATOM 715 C LYS A 123 −7.942 −15.473 −19.401 1.00 8.27 C ATOM 716 O LYS A 123 −8.261 −15.143 −20.549 1.00 9.12 O ATOM 717 CB LYS A 123 −9.684 −16.071 −17.727 1.00 10.40 C ATOM 718 CG LYS A 123 −10.695 −17.105 −17.284 1.00 13.19 C ATOM 719 CD LYS A 123 −11.481 −16.695 −16.065 1.00 16.46 C ATOM 720 CE LYS A 123 −12.251 −15.439 −16.250 1.00 20.64 C ATOM 721 NZ LYS A 123 −13.357 −15.591 −17.241 1.00 24.59 N ATOM 722 N TYR A 124 −6.964 −14.855 −18.736 1.00 8.17 N ATOM 723 CA TYR A 124 −6.396 −13.587 −19.242 1.00 8.61 C ATOM 724 C TYR A 124 −4.955 −13.663 −19.668 1.00 8.38 C ATOM 725 O TYR A 124 −4.385 −12.637 −20.081 1.00 9.11 O ATOM 726 CB TYR A 124 −6.622 −12.500 −18.194 1.00 9.12 C ATOM 727 CG TYR A 124 −8.077 −12.250 −17.935 1.00 10.17 C ATOM 728 CD1 TYR A 124 −8.865 −11.789 −18.974 1.00 12.12 C ATOM 729 CD2 TYR A 124 −8.703 −12.501 −16.716 1.00 11.43 C ATOM 730 CE1 TYR A 124 −10.221 −11.576 −18.805 1.00 15.63 C ATOM 731 CE2 TYR A 124 −10.049 −12.289 −16.580 1.00 13.12 C ATOM 732 CZ TYR A 124 −10.781 −11.838 −17.610 1.00 15.53 C ATOM 733 OH TYR A 124 −12.167 −11.632 −17.479 1.00 20.84 O ATOM 734 N GLU A 125 −4.333 −14.835 −19.561 1.00 8.10 N ATOM 735 CA GLU A 125 −2.931 −15.026 −19.994 1.00 8.35 C ATOM 736 C GLU A 125 −2.008 −14.094 −19.200 1.00 8.73 C ATOM 737 O GLU A 125 −1.310 −13.245 −19.738 1.00 8.60 O ATOM 738 CB GLU A 125 −2.755 −14.865 −21.499 1.00 9.55 C ATOM 739 CG GLU A 125 −1.452 −15.438 −22.013 1.00 11.63 C ATOM 740 CD GLU A 125 −1.270 −15.357 −23.504 1.00 14.78 C ATOM 741 OE1 GLU A 125 −1.921 −14.539 −24.097 1.00 17.12 O ATOM 742 OE2 GLU A 125 −0.414 −16.075 −24.031 1.00 22.37 O ATOM 743 N VAL A 126 −2.065 −14.298 −17.881 1.00 7.76 N ATOM 744 CA VAL A 126 −1.284 −13.483 −16.931 1.00 7.56 C ATOM 745 C VAL A 126 −0.497 −14.442 −16.054 1.00 6.91 C ATOM 746 O VAL A 126 −1.079 −15.286 −15.385 1.00 8.12 O ATOM 747 CB VAL A 126 −2.177 −12.604 −16.059 1.00 7.91 C ATOM 748 CG1 VAL A 126 −1.340 −11.835 −15.043 1.00 8.63 C ATOM 749 CG2 VAL A 126 −3.012 −11.644 −16.910 1.00 8.53 C ATOM 750 N ASN A 127 0.799 −14.260 −16.027 1.00 6.40 N ATOM 751 CA ASN A 127 1.708 −15.123 −15.221 1.00 6.47 C ATOM 752 C ASN A 127 1.286 −15.184 −13.759 1.00 6.18 C ATOM 753 O ASN A 127 1.194 −14.141 −13.095 1.00 6.41 O ATOM 754 CB ASN A 127 3.117 −14.553 −15.388 1.00 6.38 C ATOM 755 CG ASN A 127 4.191 −15.261 −14.594 1.00 7.02 C ATOM 756 OD1 ASN A 127 4.014 −15.565 −13.407 1.00 6.96 O ATOM 757 ND2 ASN A 127 5.347 −15.467 −15.216 1.00 7.65 N ATOM 758 N VAL A 128 0.961 −16.374 −13.293 1.00 6.13 N ATOM 759 CA VAL A 128 0.312 −16.475 −11.974 1.00 6.44 C ATOM 760 C VAL A 128 1.305 −16.203 −10.863 1.00 6.25 C ATOM 761 O VAL A 128 0.863 −15.740 −9.761 1.00 6.88 O ATOM 762 CB VAL A 128 −0.389 −17.840 −11.745 1.00 6.28 C ATOM 763 CG1 VAL A 128 0.614 −18.970 −11.449 1.00 7.35 C ATOM 764 CG2 VAL A 128 −1.430 −17.740 −10.630 1.00 6.53 C ATOM 765 N ILE A 129 2.595 −16.479 −11.059 1.00 5.87 N ATOM 766 CA ILE A 129 3.566 −16.120 −10.027 1.00 6.09 C ATOM 767 C ILE A 129 3.663 −14.622 −9.902 1.00 6.67 C ATOM 768 O ILE A 129 3.722 −14.067 −8.792 1.00 6.57 O ATOM 769 CB ILE A 129 4.880 −16.803 −10.245 1.00 6.67 C ATOM 770 CG1 ILE A 129 4.778 −18.290 −9.931 1.00 7.34 C ATOM 771 CG2 ILE A 129 5.959 −16.172 −9.400 1.00 7.22 C ATOM 772 CD1 ILE A 129 5.960 −19.142 −10.348 1.00 8.61 C ATOM 773 N TYR A 130 3.674 −13.883 −11.010 1.00 5.83 N ATOM 774 CA TYR A 130 3.593 −12.448 −10.969 1.00 5.97 C ATOM 775 C TYR A 130 2.343 −11.988 −10.259 1.00 6.12 C ATOM 776 O TYR A 130 2.387 −11.101 −9.397 1.00 6.25 O ATOM 777 CB TYR A 130 3.655 −11.901 −12.422 1.00 6.08 C ATOM 778 CG TYR A 130 2.947 −10.576 −12.532 1.00 6.11 C ATOM 779 CD1 TYR A 130 3.439 −9.419 −11.883 1.00 6.13 C ATOM 780 CD2 TYR A 130 1.715 −10.458 −13.141 1.00 6.36 C ATOM 781 CE1 TYR A 130 2.709 −8.259 −11.821 1.00 6.47 C ATOM 782 CE2 TYR A 130 1.023 −9.271 −13.163 1.00 7.06 C ATOM 783 CZ TYR A 130 1.498 −8.196 −12.468 1.00 6.52 C ATOM 784 OH TYR A 130 0.747 −7.026 −12.378 1.00 8.23 O ATOM 785 N LEU A 131 1.198 −12.550 −10.620 1.00 6.32 N ATOM 786 CA LEU A 131 −0.067 −12.088 −10.074 1.00 6.92 C ATOM 787 C LEU A 131 −0.103 −12.260 −8.549 1.00 6.79 C ATOM 788 O LEU A 131 −0.480 −11.342 −7.796 1.00 6.46 O ATOM 789 CB LEU A 131 −1.252 −12.804 −10.746 1.00 8.60 C ATOM 790 CG LEU A 131 −2.637 −12.189 −10.591 1.00 10.25 C ATOM 791 CD1 LEU A 131 −2.680 −10.828 −11.251 1.00 9.89 C ATOM 792 CD2 LEU A 131 −3.700 −13.123 −11.173 1.00 10.87 C ATOM 793 N VAL A 132 0.307 −13.418 −8.065 1.00 6.82 N ATOM 794 CA VAL A 132 0.388 −13.708 −6.621 1.00 7.32 C ATOM 795 C VAL A 132 1.371 −12.734 −6.000 1.00 7.43 C ATOM 796 O VAL A 132 1.058 −12.152 −4.929 1.00 7.73 O ATOM 797 CB VAL A 132 0.798 −15.166 −6.376 1.00 8.13 C ATOM 798 CG1 VAL A 132 1.247 −15.308 −4.920 1.00 8.94 C ATOM 799 CG2 VAL A 132 −0.386 −16.097 −6.698 1.00 8.79 C ATOM 800 N SER A 133 2.555 −12.532 −6.591 1.00 6.32 N ATOM 801 CA SER A 133 3.595 −11.673 −5.984 1.00 7.29 C ATOM 802 C SER A 133 3.086 −10.254 −5.904 1.00 6.98 C ATOM 803 O SER A 133 3.323 −9.580 −4.876 1.00 7.83 O ATOM 804 CB SER A 133 4.902 −11.799 −6.739 1.00 7.20 C ATOM 805 OG SER A 133 5.373 −13.128 −6.776 1.00 7.83 O ATOM 806 N HIS A 134 2.451 −9.738 −6.932 1.00 6.96 N ATOM 807 CA HIS A 134 1.921 −8.363 −6.924 1.00 7.07 C ATOM 808 C HIS A 134 0.875 −8.258 −5.807 1.00 7.51 C ATOM 809 O HIS A 134 0.929 −7.309 −4.988 1.00 7.90 O ATOM 810 CB HIS A 134 1.360 −8.085 −8.308 1.00 7.33 C ATOM 811 CG HIS A 134 0.880 −6.696 −8.503 1.00 7.71 C ATOM 812 ND1 HIS A 134 0.535 −6.202 −9.731 1.00 8.28 N ATOM 813 CD2 HIS A 134 0.738 −5.678 −7.620 1.00 8.37 C ATOM 814 CE1 HIS A 134 0.184 −4.936 −9.600 1.00 8.93 C ATOM 815 NE2 HIS A 134 0.291 −4.598 −8.334 1.00 8.59 N ATOM 816 N ALA A 135 −0.038 −9.216 −5.716 1.00 7.61 N ATOM 817 CA ALA A 135 −1.076 −9.142 −4.661 1.00 7.88 C ATOM 818 C ALA A 135 −0.439 −9.200 −3.306 1.00 8.64 C ATOM 819 O ALA A 135 −0.913 −8.475 −2.377 1.00 9.55 O ATOM 820 CB ALA A 135 −2.047 −10.275 −4.847 1.00 9.07 C ATOM 821 N LEU A 136 0.576 −9.999 −3.100 1.00 8.20 N ATOM 822 CA LEU A 136 1.184 −10.104 −1.742 1.00 9.40 C ATOM 823 C LEU A 136 1.874 −8.832 −1.372 1.00 10.49 C ATOM 824 O LEU A 136 1.816 −8.421 −0.174 1.00 12.23 O ATOM 825 CB LEU A 136 2.134 −11.241 −1.702 1.00 9.44 C ATOM 826 CG LEU A 136 1.528 −12.632 −1.753 1.00 10.07 C ATOM 827 CD1 LEU A 136 2.573 −13.735 −1.920 1.00 10.72 C ATOM 828 CD2 LEU A 136 0.672 −12.992 −0.534 1.00 11.10 C ATOM 829 N VAL A 137 2.560 −8.180 −2.296 1.00 9.88 N ATOM 830 CA VAL A 137 3.180 −6.906 −1.948 1.00 11.54 C ATOM 831 C VAL A 137 2.099 −5.913 −1.615 1.00 12.20 C ATOM 832 O VAL A 137 2.163 −5.253 −0.525 1.00 13.74 O ATOM 833 CB VAL A 137 4.101 −6.406 −3.070 1.00 11.33 C ATOM 834 CG1 VAL A 137 4.643 −5.022 −2.653 1.00 13.21 C ATOM 835 CG2 VAL A 137 5.256 −7.341 −3.269 1.00 10.79 C ATOM 836 N GLU A 138 1.084 −5.760 −2.449 1.00 10.81 N ATOM 837 CA GLU A 138 0.082 −4.712 −2.298 1.00 11.29 C ATOM 838 C GLU A 138 −0.738 −4.864 −1.052 1.00 12.33 C ATOM 839 O GLU A 138 −1.061 −3.857 −0.382 1.00 16.05 O ATOM 840 CB GLU A 138 −0.837 −4.702 −3.522 1.00 14.11 C ATOM 841 CG GLU A 138 −1.932 −3.694 −3.501 1.00 18.76 C ATOM 842 CD GLU A 138 −1.474 −2.244 −3.437 1.00 26.12 C ATOM 843 OE1 GLU A 138 −2.249 −1.483 −2.790 1.00 33.62 O ATOM 844 OE2 GLU A 138 −0.434 −1.875 −4.036 1.00 30.66 O ATOM 845 N THR A 139 −1.022 −6.083 −0.659 1.00 10.45 N ATOM 846 CA THR A 139 −1.944 −6.359 0.466 1.00 10.54 C ATOM 847 C THR A 139 −1.218 −6.630 1.777 1.00 11.10 C ATOM 848 O THR A 139 −1.828 −7.006 2.782 1.00 11.59 O ATOM 849 CB THR A 139 −2.827 −7.602 0.171 1.00 11.09 C ATOM 850 OG1 THR A 139 −2.039 −8.763 0.027 1.00 10.07 O ATOM 851 CG2 THR A 139 −3.704 −7.380 −1.039 1.00 12.72 C ATOM 852 N GLY A 140 0.112 −6.514 1.764 1.00 10.29 N ATOM 853 CA GLY A 140 0.874 −6.870 2.958 1.00 11.09 C ATOM 854 C GLY A 140 0.711 −8.318 3.319 1.00 11.29 C ATOM 855 O GLY A 140 0.288 −8.673 4.422 1.00 11.16 O ATOM 856 N ASN A 141 1.050 −9.224 2.403 1.00 12.13 N ATOM 857 CA ASN A 141 0.939 −10.635 2.670 1.00 12.49 C ATOM 858 C ASN A 141 −0.503 −11.053 2.971 1.00 11.09 C ATOM 859 O ASN A 141 −0.740 −11.974 3.748 1.00 12.32 O ATOM 860 CB ASN A 141 1.894 −11.127 3.762 1.00 14.48 C ATOM 861 CG ASN A 141 3.276 −11.436 3.236 1.00 17.63 C ATOM 862 OD1 ASN A 141 4.279 −11.278 4.008 1.00 27.51 O ATOM 863 ND2 ASN A 141 3.393 −11.789 1.982 1.00 16.25 N ATOM 864 N GLY A 142 −1.443 −10.370 2.352 1.00 10.81 N ATOM 865 CA GLY A 142 −2.851 −10.665 2.556 1.00 10.66 C ATOM 866 C GLY A 142 −3.431 −10.256 3.874 1.00 11.36 C ATOM 867 O GLY A 142 −4.605 −10.502 4.151 1.00 11.65 O ATOM 868 N LYS A 143 −2.676 −9.496 4.665 1.00 12.13 N ATOM 869 CA LYS A 143 −3.028 −9.270 6.080 1.00 13.27 C ATOM 870 C LYS A 143 −3.305 −7.835 6.389 1.00 13.02 C ATOM 871 O LYS A 143 −3.658 −7.527 7.570 1.00 16.06 O ATOM 872 CB LYS A 143 −1.950 −9.812 6.978 1.00 14.56 C ATOM 873 CG LYS A 143 −1.908 −11.334 6.998 1.00 17.20 C ATOM 874 CD LYS A 143 −0.741 −11.784 7.805 1.00 22.00 C ATOM 875 CE LYS A 143 −0.457 −13.228 7.581 1.00 27.18 C ATOM 876 NZ LYS A 143 0.975 −13.471 7.935 1.00 33.47 N ATOM 877 N SER A 144 −3.221 −6.918 5.458 1.00 12.33 N ATOM 878 CA SER A 144 −3.490 −5.496 5.773 1.00 13.51 C ATOM 879 C SER A 144 −4.967 −5.263 6.032 1.00 15.16 C ATOM 880 O SER A 144 −5.853 −6.004 5.572 1.00 14.24 O ATOM 881 CB SER A 144 −3.076 −4.594 4.616 1.00 13.23 C ATOM 882 OG SER A 144 −3.937 −4.675 3.505 1.00 13.70 O ATOM 883 N GLU A 145 −5.277 −4.208 6.797 1.00 16.08 N ATOM 884 CA GLU A 145 −6.659 −3.855 6.991 1.00 17.75 C ATOM 885 C GLU A 145 −7.376 −3.514 5.680 1.00 16.15 C ATOM 886 O GLU A 145 −8.512 −3.913 5.444 1.00 15.69 O ATOM 887 CB GLU A 145 −6.757 −2.675 7.974 1.00 23.35 C ATOM 888 CG GLU A 145 −8.212 −2.345 8.338 1.00 28.95 C ATOM 889 CD GLU A 145 −8.858 −3.377 9.258 1.00 36.54 C ATOM 890 OE1 GLU A 145 −8.267 −4.464 9.527 1.00 45.69 O ATOM 891 OE2 GLU A 145 −9.993 −3.102 9.743 1.00 50.72 O ATOM 892 N LEU A 146 −6.722 −2.772 4.788 1.00 15.60 N ATOM 893 CA LEU A 146 −7.322 −2.422 3.538 1.00 16.16 C ATOM 894 C LEU A 146 −7.670 −3.637 2.665 1.00 12.77 C ATOM 895 O LEU A 146 −8.596 −3.577 1.888 1.00 13.41 O ATOM 896 CB LEU A 146 −6.405 −1.463 2.772 1.00 19.73 C ATOM 897 CG LEU A 146 −6.919 −0.841 1.526 1.00 23.19 C ATOM 898 CD1 LEU A 146 −8.126 0.043 1.846 1.00 25.94 C ATOM 899 CD2 LEU A 146 −5.776 −0.021 0.892 1.00 26.74 C ATOM 900 N ALA A 147 −6.885 −4.688 2.795 1.00 12.41 N ATOM 901 CA ALA A 147 −7.181 −5.882 1.993 1.00 11.19 C ATOM 902 C ALA A 147 −8.534 −6.478 2.365 1.00 11.15 C ATOM 903 O ALA A 147 −9.123 −7.123 1.554 1.00 10.39 O ATOM 904 CB ALA A 147 −6.104 −6.892 2.179 1.00 10.71 C ATOM 905 N LYS A 148 −9.062 −6.152 3.556 1.00 10.90 N ATOM 906 CA LYS A 148 −10.379 −6.593 3.937 1.00 11.03 C ATOM 907 C LYS A 148 −11.499 −5.752 3.377 1.00 11.21 C ATOM 908 O LYS A 148 −12.669 −6.008 3.670 1.00 12.03 O ATOM 909 CB LYS A 148 −10.455 −6.786 5.474 1.00 12.65 C ATOM 910 CG LYS A 148 −9.477 −7.832 6.010 1.00 14.18 C ATOM 911 CD LYS A 148 −9.693 −8.280 7.474 1.00 15.45 C ATOM 912 CE LYS A 148 −8.492 −9.006 8.039 1.00 18.31 C ATOM 913 NZ LYS A 148 −7.899 −10.133 7.271 1.00 19.78 N ATOM 914 N GLY A 149 −11.203 −4.740 2.544 1.00 11.24 N ATOM 915 CA GLY A 149 −12.211 −4.146 1.752 1.00 11.90 C ATOM 916 C GLY A 149 −12.854 −2.896 2.355 1.00 12.37 C ATOM 917 O GLY A 149 −12.540 −2.552 3.480 1.00 15.68 O ATOM 918 N ILE A 150 −13.691 −2.300 1.562 1.00 13.35 N ATOM 919 CA ILE A 150 −14.362 −1.014 1.922 1.00 14.97 C ATOM 920 C ILE A 150 −15.851 −1.198 1.753 1.00 14.74 C ATOM 921 O ILE A 150 −16.361 −1.619 0.748 1.00 14.07 O ATOM 922 CB ILE A 150 −13.872 0.119 1.029 1.00 17.38 C ATOM 923 CG1 ILE A 150 −12.403 0.327 1.233 1.00 18.09 C ATOM 924 CG2 ILE A 150 −14.601 1.447 1.352 1.00 18.80 C ATOM 925 CD1 ILE A 150 −11.797 1.178 0.122 1.00 21.67 C ATOM 926 N LYS A 151 −16.564 −0.856 2.828 1.00 17.86 N ATOM 927 CA LYS A 151 −18.003 −1.022 2.888 1.00 20.82 C ATOM 928 C LYS A 151 −18.715 0.091 2.145 1.00 21.11 C ATOM 929 O LYS A 151 −18.257 1.230 2.174 1.00 22.12 O ATOM 930 CB LYS A 151 −18.426 −1.051 4.359 1.00 25.53 C ATOM 931 CG LYS A 151 −19.879 −1.383 4.585 1.00 33.29 C ATOM 932 CD LYS A 151 −20.210 −1.329 6.073 1.00 38.11 C ATOM 933 CE LYS A 151 −21.700 −1.540 6.328 1.00 42.75 C ATOM 934 NZ LYS A 151 −22.547 −0.510 5.646 1.00 46.33 N ATOM 935 N ASP A 152 −19.768 −0.263 1.423 1.00 20.28 N ATOM 936 CA ASP A 152 −20.715 0.718 0.876 1.00 24.19 C ATOM 937 C ASP A 152 −22.078 0.076 0.741 1.00 24.13 C ATOM 938 O ASP A 152 −22.295 −0.884 −0.022 1.00 23.13 O ATOM 939 CB ASP A 152 −20.246 1.261 −0.467 1.00 29.52 C ATOM 940 CG ASP A 152 −21.141 2.388 −0.965 1.00 34.23 C ATOM 941 OD1 ASP A 152 −21.153 3.467 −0.337 1.00 39.62 O ATOM 942 OD2 ASP A 152 −21.829 2.176 −1.970 1.00 39.95 O ATOM 943 N GLY A 153 −23.025 0.606 1.519 1.00 26.24 N ATOM 944 CA GLY A 153 −24.350 0.000 1.627 1.00 23.41 C ATOM 945 C GLY A 153 −24.229 −1.411 2.162 1.00 21.65 C ATOM 946 O GLY A 153 −23.569 −1.650 3.146 1.00 25.00 O ATOM 947 N LYS A 154 −24.859 −2.350 1.493 1.00 19.76 N ATOM 948 CA LYS A 154 −24.802 −3.728 1.956 1.00 20.22 C ATOM 949 C LYS A 154 −23.669 −4.548 1.363 1.00 17.98 C ATOM 950 O LYS A 154 −23.557 −5.748 1.630 1.00 16.13 O ATOM 951 CB LYS A 154 −26.153 −4.379 1.728 1.00 21.58 C ATOM 952 CG LYS A 154 −27.230 −3.562 2.457 1.00 26.02 C ATOM 953 CD LYS A 154 −28.248 −4.410 3.188 1.00 27.45 C ATOM 954 CE LYS A 154 −29.439 −3.547 3.651 1.00 26.37 C ATOM 955 NZ LYS A 154 −30.388 −4.302 4.526 1.00 28.16 N ATOM 956 N LYS A 155 −22.811 −3.887 0.588 1.00 17.05 N ATOM 957 CA LYS A 155 −21.715 −4.552 −0.101 1.00 16.46 C ATOM 958 C LYS A 155 −20.375 −4.141 0.461 1.00 16.14 C ATOM 959 O LYS A 155 −20.229 −3.158 1.176 1.00 17.69 O ATOM 960 CB LYS A 155 −21.787 −4.197 −1.588 1.00 20.02 C ATOM 961 CG LYS A 155 −23.029 −4.708 −2.317 1.00 23.11 C ATOM 962 CD LYS A 155 −23.071 −4.070 −3.686 1.00 28.72 C ATOM 963 CE LYS A 155 −24.201 −4.616 −4.528 1.00 35.93 C ATOM 964 NZ LYS A 155 −24.423 −3.693 −5.679 1.00 40.80 N ATOM 965 N ARG A 156 −19.346 −4.925 0.152 1.00 15.35 N ATOM 966 CA ARG A 156 −17.956 −4.569 0.463 1.00 14.92 C ATOM 967 C ARG A 156 −17.225 −4.716 −0.831 1.00 12.58 C ATOM 968 O ARG A 156 −17.438 −5.711 −1.557 1.00 12.90 O ATOM 969 CB ARG A 156 −17.313 −5.480 1.496 1.00 18.24 C ATOM 970 CG ARG A 156 −17.796 −5.241 2.876 1.00 22.55 C ATOM 971 CD ARG A 156 −16.963 −5.956 3.892 1.00 25.24 C ATOM 972 NE ARG A 156 −17.554 −5.738 5.197 1.00 31.63 N ATOM 973 CZ ARG A 156 −16.966 −6.024 6.360 1.00 37.60 C ATOM 974 NH1 ARG A 156 −15.748 −6.568 6.409 1.00 36.26 N ATOM 975 NH2 ARG A 156 −17.615 −5.757 7.494 1.00 40.08 N ATOM 976 N TYR A 157 −16.316 −3.769 −1.076 1.00 11.89 N ATOM 977 CA TYR A 157 −15.473 −3.788 −2.283 1.00 11.56 C ATOM 978 C TYR A 157 −14.040 −4.108 −1.891 1.00 9.70 C ATOM 979 O TYR A 157 −13.526 −3.692 −0.885 1.00 10.77 O ATOM 980 CB TYR A 157 −15.578 −2.464 −3.026 1.00 13.28 C ATOM 981 CG TYR A 157 −17.000 −2.255 −3.503 1.00 15.44 C ATOM 982 CD1 TYR A 157 −17.480 −2.822 −4.659 1.00 15.95 C ATOM 983 CD2 TYR A 157 −17.903 −1.584 −2.701 1.00 19.95 C ATOM 984 CE1 TYR A 157 −18.820 −2.691 −5.060 1.00 18.07 C ATOM 985 CE2 TYR A 157 −19.240 −1.454 −3.097 1.00 21.45 C ATOM 986 CZ TYR A 157 −19.665 −1.972 −4.266 1.00 20.91 C ATOM 987 OH TYR A 157 −20.986 −1.804 −4.709 1.00 26.55 O ATOM 988 N TYR A 158 −13.417 −4.901 −2.784 1.00 9.40 N ATOM 989 CA TYR A 158 −12.057 −5.442 −2.564 1.00 9.30 C ATOM 990 C TYR A 158 −11.163 −5.012 −3.713 1.00 8.23 C ATOM 991 O TYR A 158 −11.618 −4.833 −4.832 1.00 9.33 O ATOM 992 CB TYR A 158 −12.094 −6.982 −2.466 1.00 9.29 C ATOM 993 CG TYR A 158 −12.879 −7.452 −1.274 1.00 8.71 C ATOM 994 CD1 TYR A 158 −14.264 −7.601 −1.396 1.00 8.89 C ATOM 995 CD2 TYR A 158 −12.287 −7.761 −0.083 1.00 8.75 C ATOM 996 CE1 TYR A 158 −15.026 −7.999 −0.286 1.00 9.56 C ATOM 997 CE2 TYR A 158 −13.045 −8.144 1.027 1.00 9.82 C ATOM 998 CZ TYR A 158 −14.410 −8.282 0.880 1.00 9.26 C ATOM 999 OH TYR A 158 −15.168 −8.694 1.984 1.00 10.35 O ATOM 1000 N ASN A 159 −9.873 −4.898 −3.403 1.00 8.55 N ATOM 1001 CA ASN A 159 −8.885 −4.478 −4.445 1.00 8.39 C ATOM 1002 C ASN A 159 −7.540 −4.913 −3.957 1.00 8.49 C ATOM 1003 O ASN A 159 −7.052 −4.491 −2.946 1.00 9.94 O ATOM 1004 CB ASN A 159 −8.952 −2.938 −4.557 1.00 9.24 C ATOM 1005 CG ASN A 159 −8.151 −2.366 −5.735 1.00 10.96 C ATOM 1006 OD1 ASN A 159 −7.182 −2.958 −6.236 1.00 11.33 O ATOM 1007 ND2 ASN A 159 −8.595 −1.204 −6.207 1.00 11.67 N ATOM 1008 N PHE A 160 −6.963 −5.873 −4.692 1.00 8.09 N ATOM 1009 CA PHE A 160 −5.699 −6.465 −4.289 1.00 7.56 C ATOM 1010 C PHE A 160 −4.462 −6.028 −5.090 1.00 7.63 C ATOM 1011 O PHE A 160 −3.375 −6.557 −4.895 1.00 8.90 O ATOM 1012 CB PHE A 160 −5.782 −8.001 −4.293 1.00 8.01 C ATOM 1013 CG PHE A 160 −6.872 −8.574 −3.389 1.00 8.64 C ATOM 1014 CD1 PHE A 160 −7.174 −8.014 −2.174 1.00 8.60 C ATOM 1015 CD2 PHE A 160 −7.562 −9.718 −3.770 1.00 8.92 C ATOM 1016 CE1 PHE A 160 −8.185 −8.561 −1.371 1.00 9.32 C ATOM 1017 CE2 PHE A 160 −8.514 −10.284 −2.957 1.00 9.63 C ATOM 1018 CZ PHE A 160 −8.832 −9.707 −1.776 1.00 8.98 C ATOM 1019 N PHE A 161 −4.668 −5.093 −5.999 1.00 7.82 N ATOM 1020 CA PHE A 161 −3.596 −4.673 −6.919 1.00 8.40 C ATOM 1021 C PHE A 161 −3.384 −3.186 −6.983 1.00 9.78 C ATOM 1022 O PHE A 161 −2.748 −2.732 −7.947 1.00 11.10 O ATOM 1023 CB PHE A 161 −3.825 −5.316 −8.294 1.00 8.17 C ATOM 1024 CG PHE A 161 −3.926 −6.820 −8.265 1.00 7.60 C ATOM 1025 CD1 PHE A 161 −2.804 −7.634 −8.279 1.00 7.97 C ATOM 1026 CD2 PHE A 161 −5.162 −7.410 −8.220 1.00 7.70 C ATOM 1027 CE1 PHE A 161 −2.913 −9.002 −8.209 1.00 7.97 C ATOM 1028 CE2 PHE A 161 −5.287 −8.776 −8.160 1.00 8.28 C ATOM 1029 CZ PHE A 161 −4.172 −9.604 −8.163 1.00 8.10 C ATOM 1030 N GLY A 162 −3.950 −2.422 −6.066 1.00 9.69 N ATOM 1031 CA GLY A 162 −3.723 −0.984 −6.148 1.00 10.72 C ATOM 1032 C GLY A 162 −4.265 −0.345 −7.368 1.00 11.64 C ATOM 1033 O GLY A 162 −3.643 0.609 −7.897 1.00 13.66 O ATOM 1034 N ILE A 163 −5.400 −0.790 −7.873 1.00 10.29 N ATOM 1035 CA ILE A 163 −6.001 −0.272 −9.081 1.00 10.88 C ATOM 1036 C ILE A 163 −6.971 0.830 −8.796 1.00 12.62 C ATOM 1037 O ILE A 163 −8.022 0.664 −8.163 1.00 12.13 O ATOM 1038 CB ILE A 163 −6.675 −1.398 −9.862 1.00 10.52 C ATOM 1039 CG1 ILE A 163 −5.649 −2.480 −10.253 1.00 10.49 C ATOM 1040 CG2 ILE A 163 −7.360 −0.811 −11.068 1.00 11.06 C ATOM 1041 CD1 ILE A 163 −6.256 −3.790 −10.654 1.00 10.53 C ATOM 1042 N GLY A 164 −6.601 2.016 −9.305 1.00 14.03 N ATOM 1043 CA GLY A 164 −7.460 3.204 −9.136 1.00 15.22 C ATOM 1044 C GLY A 164 −7.522 3.702 −7.735 1.00 16.47 C ATOM 1045 O GLY A 164 −6.713 3.349 −6.890 1.00 18.13 O ATOM 1046 N ALA A 165 −8.502 4.589 −7.475 1.00 17.40 N ATOM 1047 CA ALA A 165 −8.654 5.094 −6.135 1.00 17.47 C ATOM 1048 C ALA A 165 −9.448 4.101 −5.282 1.00 15.06 C ATOM 1049 O ALA A 165 −10.456 3.586 −5.703 1.00 17.71 O ATOM 1050 CB ALA A 165 −9.337 6.460 −6.119 1.00 17.86 C ATOM 1051 N PHE A 166 −8.880 3.858 −4.130 1.00 17.68 N ATOM 1052 CA PHE A 166 −9.415 2.860 −3.191 1.00 16.94 C ATOM 1053 C PHE A 166 −9.288 3.271 −1.742 1.00 18.64 C ATOM 1054 O PHE A 166 −8.362 2.900 −1.037 1.00 21.06 O ATOM 1055 CB PHE A 166 −8.788 1.493 −3.488 1.00 15.21 C ATOM 1056 CG PHE A 166 −9.544 0.336 −2.806 1.00 13.54 C ATOM 1057 CD1 PHE A 166 −10.775 −0.014 −3.271 1.00 13.14 C ATOM 1058 CD2 PHE A 166 −9.011 −0.324 −1.759 1.00 13.60 C ATOM 1059 CE1 PHE A 166 −11.525 −1.067 −2.656 1.00 14.01 C ATOM 1060 CE2 PHE A 166 −9.727 −1.406 −1.143 1.00 14.07 C ATOM 1061 CZ PHE A 166 −10.948 −1.725 −1.598 1.00 12.66 C ATOM 1062 N ASP A 167 −10.282 4.076 −1.355 1.00 24.94 N ATOM 1063 CA ASP A 167 −10.501 4.512 0.031 1.00 26.64 C ATOM 1064 C ASP A 167 −11.994 4.805 0.207 1.00 24.99 C ATOM 1065 O ASP A 167 −12.767 4.782 −0.754 1.00 26.37 O ATOM 1066 CB ASP A 167 −9.626 5.728 0.360 1.00 30.81 C ATOM 1067 CG ASP A 167 −9.850 6.900 −0.569 1.00 32.54 C ATOM 1068 OD1 ASP A 167 −10.994 7.194 −0.968 1.00 35.13 O ATOM 1069 OD2 ASP A 167 −8.846 7.553 −0.909 1.00 40.97 O ATOM 1070 N SER A 168 −12.401 5.113 1.452 1.00 31.69 N ATOM 1071 CA SER A 168 −13.814 5.344 1.755 1.00 33.30 C ATOM 1072 C SER A 168 −14.430 6.488 0.989 1.00 32.40 C ATOM 1073 O SER A 168 −15.510 6.317 0.454 1.00 32.86 O ATOM 1074 CB SER A 168 −14.041 5.541 3.254 1.00 39.75 C ATOM 1075 OG SER A 168 −13.655 4.368 3.945 1.00 45.60 O ATOM 1076 N SER A 169 −13.724 7.617 0.875 1.00 35.48 N ATOM 1077 CA SER A 169 −14.211 8.741 0.063 1.00 37.10 C ATOM 1078 C SER A 169 −14.528 8.277 −1.348 1.00 35.55 C ATOM 1079 O SER A 169 −15.660 8.437 −1.825 1.00 34.19 O ATOM 1080 CB SER A 169 −13.183 9.886 −0.015 1.00 41.12 C ATOM 1081 OG SER A 169 −12.698 10.244 1.261 1.00 45.25 O ATOM 1082 N ALA A 170 −13.536 7.653 −2.006 1.00 31.96 N ATOM 1083 CA ALA A 170 −13.673 7.293 −3.414 1.00 30.78 C ATOM 1084 C ALA A 170 −14.780 6.283 −3.612 1.00 27.53 C ATOM 1085 O ALA A 170 −15.539 6.338 −4.569 1.00 31.58 O ATOM 1086 CB ALA A 170 −12.347 6.748 −3.955 1.00 26.95 C ATOM 1087 N VAL A 171 −14.865 5.324 −2.694 1.00 32.42 N ATOM 1088 CA VAL A 171 −15.908 4.299 −2.757 1.00 35.74 C ATOM 1089 C VAL A 171 −17.299 4.919 −2.497 1.00 36.55 C ATOM 1090 O VAL A 171 −18.227 4.671 −3.260 1.00 35.20 O ATOM 1091 CB VAL A 171 −15.626 3.113 −1.786 1.00 34.33 C ATOM 1092 CG1 VAL A 171 −16.817 2.154 −1.764 1.00 32.75 C ATOM 1093 CG2 VAL A 171 −14.375 2.364 −2.217 1.00 33.40 C ATOM 1094 N ARG A 172 −17.424 5.765 −1.472 1.00 45.16 N ATOM 1095 CA ARG A 172 −18.707 6.466 −1.209 1.00 50.95 C ATOM 1096 C ARG A 172 −19.191 7.307 −2.412 1.00 53.17 C ATOM 1097 O ARG A 172 −20.372 7.251 −2.776 1.00 54.99 O ATOM 1098 CB ARG A 172 −18.610 7.332 0.052 1.00 56.55 C ATOM 1099 CG ARG A 172 −18.498 6.531 1.350 1.00 61.00 C ATOM 1100 CD ARG A 172 −18.742 7.399 2.581 1.00 65.06 C ATOM 1101 NE ARG A 172 −17.931 8.626 2.583 1.00 67.07 N ATOM 1102 CZ ARG A 172 −16.814 8.832 3.291 1.00 69.01 C ATOM 1103 NH1 ARG A 172 −16.182 10.002 3.193 1.00 70.62 N ATOM 1104 NH2 ARG A 172 −16.316 7.896 4.097 1.00 67.75 N ATOM 1105 N SER A 173 −18.276 8.045 −3.047 1.00 49.50 N ATOM 1106 CA SER A 173 −18.622 8.909 −4.184 1.00 50.52 C ATOM 1107 C SER A 173 −18.805 8.185 −5.526 1.00 50.89 C ATOM 1108 O SER A 173 −19.270 8.792 −6.489 1.00 54.62 O ATOM 1109 CB SER A 173 −17.529 9.953 −4.365 1.00 52.02 C ATOM 1110 OG SER A 173 −16.328 9.332 −4.795 1.00 50.30 O ATOM 1111 N GLY A 174 −18.403 6.915 −5.601 1.00 47.33 N ATOM 1112 CA GLY A 174 −18.498 6.139 −6.836 1.00 44.83 C ATOM 1113 C GLY A 174 −17.382 6.489 −7.811 1.00 43.77 C ATOM 1114 O GLY A 174 −17.451 6.124 −8.975 1.00 43.92 O ATOM 1115 N LYS A 175 −16.350 7.187 −7.340 1.00 38.42 N ATOM 1116 CA LYS A 175 −15.156 7.460 −8.149 1.00 39.75 C ATOM 1117 C LYS A 175 −14.095 6.422 −7.778 1.00 34.68 C ATOM 1118 O LYS A 175 −12.921 6.761 −7.542 1.00 37.72 O ATOM 1119 CB LYS A 175 −14.655 8.894 −7.920 1.00 47.12 C ATOM 1120 CG LYS A 175 −15.343 9.934 −8.809 1.00 52.60 C ATOM 1121 CD LYS A 175 −15.294 11.336 −8.214 1.00 56.75 C ATOM 1122 CE LYS A 175 −16.590 11.659 −7.484 1.00 61.98 C ATOM 1123 NZ LYS A 175 −16.512 12.866 −6.609 1.00 65.10 N ATOM 1124 N SER A 176 −14.529 5.165 −7.688 1.00 27.00 N ATOM 1125 CA SER A 176 −13.615 4.034 −7.381 1.00 23.61 C ATOM 1126 C SER A 176 −13.791 2.912 −8.382 1.00 19.11 C ATOM 1127 O SER A 176 −14.878 2.396 −8.620 1.00 17.36 O ATOM 1128 CB SER A 176 −13.821 3.532 −5.959 1.00 23.47 C ATOM 1129 OG SER A 176 −13.074 2.349 −5.717 1.00 24.64 O ATOM 1130 N TYR A 177 −12.672 2.529 −9.002 1.00 17.68 N ATOM 1131 CA TYR A 177 −12.682 1.525 −10.026 1.00 15.76 C ATOM 1132 C TYR A 177 −13.215 0.165 −9.499 1.00 14.04 C ATOM 1133 O TYR A 177 −13.989 −0.499 −10.164 1.00 14.24 O ATOM 1134 CB TYR A 177 −11.278 1.333 −10.627 1.00 17.64 C ATOM 1135 CG TYR A 177 −11.296 0.595 −11.926 1.00 18.62 C ATOM 1136 CD1 TYR A 177 −11.660 1.239 −13.095 1.00 21.83 C ATOM 1137 CD2 TYR A 177 −10.916 −0.740 −12.010 1.00 18.42 C ATOM 1138 CE1 TYR A 177 −11.659 0.586 −14.306 1.00 22.58 C ATOM 1139 CE2 TYR A 177 −10.912 −1.409 −13.207 1.00 20.88 C ATOM 1140 CZ TYR A 177 −11.269 −0.734 −14.373 1.00 21.36 C ATOM 1141 OH TYR A 177 −11.263 −1.405 −15.543 1.00 26.71 O ATOM 1142 N ALA A 178 −12.817 −0.128 −8.268 1.00 14.26 N ATOM 1143 CA ALA A 178 −13.277 −1.382 −7.637 1.00 14.75 C ATOM 1144 C ALA A 178 −14.795 −1.447 −7.567 1.00 15.07 C ATOM 1145 O ALA A 178 −15.403 −2.496 −7.749 1.00 14.79 O ATOM 1146 CB ALA A 178 −12.666 −1.605 −6.291 1.00 14.19 C ATOM 1147 N GLU A 179 −15.403 −0.317 −7.256 1.00 15.92 N ATOM 1148 CA GLU A 179 −16.872 −0.221 −7.221 1.00 18.91 C ATOM 1149 C GLU A 179 −17.497 −0.228 −8.618 1.00 18.17 C ATOM 1150 O GLU A 179 −18.456 −0.940 −8.861 1.00 18.43 O ATOM 1151 CB GLU A 179 −17.238 1.044 −6.453 1.00 21.29 C ATOM 1152 CG GLU A 179 −18.723 1.238 −6.255 1.00 27.74 C ATOM 1153 CD GLU A 179 −19.029 2.270 −5.181 1.00 34.74 C ATOM 1154 OE1 GLU A 179 −18.168 3.144 −4.891 1.00 41.21 O ATOM 1155 OE2 GLU A 179 −20.156 2.194 −4.650 1.00 45.93 O ATOM 1156 N LYS A 180 −16.888 0.506 −9.561 1.00 18.03 N ATOM 1157 CA LYS A 180 −17.356 0.525 −10.923 1.00 19.60 C ATOM 1158 C LYS A 180 −17.394 −0.873 −11.535 1.00 19.34 C ATOM 1159 O LYS A 180 −18.332 −1.252 −12.213 1.00 20.03 O ATOM 1160 CB LYS A 180 −16.483 1.485 −11.733 1.00 24.42 C ATOM 1161 CG LYS A 180 −16.894 1.665 −13.168 1.00 31.57 C ATOM 1162 CD LYS A 180 −16.343 2.988 −13.705 1.00 36.49 C ATOM 1163 CE LYS A 180 −16.112 2.940 −15.206 1.00 41.85 C ATOM 1164 NZ LYS A 180 −14.840 2.218 −15.509 1.00 42.37 N ATOM 1165 N GLU A 181 −16.373 −1.687 −11.254 1.00 15.93 N ATOM 1166 CA GLU A 181 −16.318 −3.032 −11.772 1.00 17.03 C ATOM 1167 C GLU A 181 −16.953 −4.097 −10.853 1.00 15.53 C ATOM 1168 O GLU A 181 −16.936 −5.283 −11.146 1.00 17.03 O ATOM 1169 CB GLU A 181 −14.833 −3.397 −12.025 1.00 18.52 C ATOM 1170 CG GLU A 181 −14.189 −2.555 −13.122 1.00 21.26 C ATOM 1171 CD GLU A 181 −14.937 −2.628 −14.425 1.00 26.39 C ATOM 1172 OE1 GLU A 181 −15.319 −3.744 −14.846 1.00 33.15 O ATOM 1173 OE2 GLU A 181 −15.125 −1.565 −15.023 1.00 33.72 O ATOM 1174 N GLN A 182 −17.534 −3.636 −9.766 1.00 15.01 N ATOM 1175 CA GLN A 182 −18.278 −4.510 −8.846 1.00 16.69 C ATOM 1176 C GLN A 182 −17.439 −5.660 −8.315 1.00 14.18 C ATOM 1177 O GLN A 182 −17.834 −6.826 −8.352 1.00 14.46 O ATOM 1178 CB GLN A 182 −19.628 −4.965 −9.451 1.00 19.53 C ATOM 1179 CG GLN A 182 −20.611 −3.785 −9.557 1.00 21.50 C ATOM 1180 CD GLN A 182 −21.036 −3.184 −8.190 1.00 23.84 C ATOM 1181 OE1 GLN A 182 −20.793 −2.013 −7.876 1.00 29.75 O ATOM 1182 NE2 GLN A 182 −21.637 −4.005 −7.368 1.00 24.83 N ATOM 1183 N TRP A 183 −16.259 −5.282 −7.781 1.00 12.49 N ATOM 1184 CA TRP A 183 −15.383 −6.248 −7.146 1.00 11.05 C ATOM 1185 C TRP A 183 −15.876 −6.434 −5.717 1.00 9.90 C ATOM 1186 O TRP A 183 −15.290 −5.980 −4.732 1.00 10.60 O ATOM 1187 CB TRP A 183 −13.921 −5.816 −7.127 1.00 10.73 C ATOM 1188 CG TRP A 183 −13.342 −5.732 −8.547 1.00 11.01 C ATOM 1189 CD1 TRP A 183 −13.852 −6.271 −9.703 1.00 10.93 C ATOM 1190 CD2 TRP A 183 −12.108 −5.113 −8.900 1.00 9.86 C ATOM 1191 NE1 TRP A 183 −13.037 −5.957 −10.771 1.00 11.53 N ATOM 1192 CE2 TRP A 183 −11.971 −5.238 −10.283 1.00 10.19 C ATOM 1193 CE3 TRP A 183 −11.163 −4.385 −8.179 1.00 10.00 C ATOM 1194 CZ2 TRP A 183 −10.890 −4.681 −10.957 1.00 9.45 C ATOM 1195 CZ3 TRP A 183 −10.114 −3.849 −8.853 1.00 9.69 C ATOM 1196 CH2 TRP A 183 −9.992 −4.015 −10.234 1.00 9.69 C ATOM 1197 N THR A 184 −16.969 −7.190 −5.611 1.00 10.93 N ATOM 1198 CA THR A 184 −17.701 −7.328 −4.356 1.00 10.93 C ATOM 1199 C THR A 184 −17.319 −8.551 −3.529 1.00 11.44 C ATOM 1200 O THR A 184 −17.898 −8.865 −2.496 1.00 11.45 O ATOM 1201 CB THR A 184 −19.231 −7.427 −4.679 1.00 12.83 C ATOM 1202 OG1 THR A 184 −19.459 −8.458 −5.624 1.00 13.84 O ATOM 1203 CG2 THR A 184 −19.736 −6.093 −5.212 1.00 14.48 C ATOM 1204 N SER A 185 −16.247 −9.233 −3.938 1.00 10.11 N ATOM 1205 CA SER A 185 −15.678 −10.357 −3.203 1.00 9.58 C ATOM 1206 C SER A 185 −14.180 −10.467 −3.484 1.00 8.62 C ATOM 1207 O SER A 185 −13.718 −9.924 −4.503 1.00 9.66 O ATOM 1208 CB SER A 185 −16.333 −11.679 −3.606 1.00 10.12 C ATOM 1209 OG SER A 185 −15.968 −12.049 −4.938 1.00 10.44 O ATOM 1210 N PRO A 186 −13.446 −11.170 −2.614 1.00 8.46 N ATOM 1211 CA PRO A 186 −12.015 −11.416 −2.885 1.00 8.89 C ATOM 1212 C PRO A 186 −11.836 −12.099 −4.214 1.00 8.80 C ATOM 1213 O PRO A 186 −10.929 −11.699 −5.006 1.00 9.12 O ATOM 1214 CB PRO A 186 −11.600 −12.270 −1.728 1.00 8.25 C ATOM 1215 CG PRO A 186 −12.497 −11.802 −0.583 1.00 8.40 C ATOM 1216 CD PRO A 186 −13.788 −11.629 −1.240 1.00 8.23 C ATOM 1217 N ASP A 187 −12.652 −13.085 −4.574 1.00 8.01 N ATOM 1218 CA ASP A 187 −12.428 −13.783 −5.832 1.00 8.22 C ATOM 1219 C ASP A 187 −12.650 −12.869 −7.012 1.00 8.43 C ATOM 1220 O ASP A 187 −11.894 −12.896 −8.002 1.00 8.56 O ATOM 1221 CB ASP A 187 −13.318 −15.014 −5.939 1.00 9.31 C ATOM 1222 CG ASP A 187 −12.942 −16.109 −4.943 1.00 10.89 C ATOM 1223 OD1 ASP A 187 −11.874 −16.075 −4.303 1.00 11.15 O ATOM 1224 OD2 ASP A 187 −13.752 −17.100 −4.803 1.00 13.42 O ATOM 1225 N LYS A 188 −13.693 −12.031 −6.959 1.00 8.19 N ATOM 1226 CA LYS A 188 −13.918 −11.049 −8.003 1.00 9.47 C ATOM 1227 C LYS A 188 −12.782 −10.047 −8.147 1.00 8.94 C ATOM 1228 O LYS A 188 −12.449 −9.665 −9.292 1.00 9.02 O ATOM 1229 CB LYS A 188 −15.247 −10.306 −7.830 1.00 11.21 C ATOM 1230 CG LYS A 188 −16.424 −11.196 −8.098 1.00 12.70 C ATOM 1231 CD LYS A 188 −17.747 −10.547 −7.607 1.00 13.92 C ATOM 1232 CE LYS A 188 −18.941 −11.450 −7.950 1.00 17.18 C ATOM 1233 NZ LYS A 188 −19.253 −11.330 −9.398 1.00 21.83 N ATOM 1234 N ALA A 189 −12.164 −9.671 −7.040 1.00 8.88 N ATOM 1235 CA ALA A 189 −10.997 −8.759 −7.084 1.00 8.58 C ATOM 1236 C ALA A 189 −9.778 −9.425 −7.710 1.00 7.82 C ATOM 1237 O ALA A 189 −8.972 −8.760 −8.377 1.00 8.05 O ATOM 1238 CB ALA A 189 −10.682 −8.228 −5.719 1.00 8.69 C ATOM 1239 N ILE A 190 −9.638 −10.719 −7.526 1.00 7.26 N ATOM 1240 CA ILE A 190 −8.480 −11.424 −8.137 1.00 7.28 C ATOM 1241 C ILE A 190 −8.666 −11.546 −9.632 1.00 8.15 C ATOM 1242 O ILE A 190 −7.788 −11.129 −10.404 1.00 8.51 O ATOM 1243 CB ILE A 190 −8.318 −12.835 −7.506 1.00 7.38 C ATOM 1244 CG1 ILE A 190 −7.898 −12.732 −6.027 1.00 7.57 C ATOM 1245 CG2 ILE A 190 −7.285 −13.640 −8.261 1.00 7.56 C ATOM 1246 CD1 ILE A 190 −8.181 −14.002 −5.234 1.00 7.32 C ATOM 1247 N ILE A 191 −9.835 −12.016 −10.077 1.00 7.82 N ATOM 1248 CA ILE A 191 −10.135 −12.116 −11.522 1.00 9.42 C ATOM 1249 C ILE A 191 −10.102 −10.741 −12.129 1.00 8.62 C ATOM 1250 O ILE A 191 −9.516 −10.543 −13.215 1.00 8.61 O ATOM 1251 CB ILE A 191 −11.523 −12.753 −11.765 1.00 11.52 C ATOM 1252 CG1 ILE A 191 −11.544 −14.158 −11.219 1.00 14.38 C ATOM 1253 CG2 ILE A 191 −11.914 −12.697 −13.239 1.00 12.42 C ATOM 1254 CD1 ILE A 191 −10.574 −15.094 −11.826 1.00 14.62 C ATOM 1255 N ILE A 192 −10.714 −9.762 −11.472 1.00 8.05 N ATOM 1256 CA GLY A 192 −10.817 −8.450 −12.060 1.00 8.45 C ATOM 1257 C GLY A 192 −9.491 −7.730 −12.165 1.00 8.36 C ATOM 1258 O GLY A 192 −9.212 −7.003 −13.135 1.00 8.84 O ATOM 1259 N GLY A 193 −8.637 −7.941 −11.168 1.00 7.86 N ATOM 1260 CA GLY A 193 −7.290 −7.366 −11.209 1.00 8.01 C ATOM 1261 C GLY A 193 −6.515 −7.890 −12.408 1.00 7.68 C ATOM 1262 O GLY A 193 −5.895 −7.126 −13.150 1.00 7.95 O ATOM 1263 N ALA A 194 −6.556 −9.201 −12.624 1.00 7.52 N ATOM 1264 CA ALA A 194 −5.900 −9.806 −13.769 1.00 7.51 C ATOM 1265 C ALA A 194 −6.458 −9.274 −15.068 1.00 7.54 C ATOM 1266 O ALA A 194 −5.692 −8.991 −16.017 1.00 7.40 O ATOM 1267 CB ALA A 194 −6.006 −11.329 −13.715 1.00 7.98 C ATOM 1268 N LYS A 195 −7.776 −9.137 −15.188 1.00 7.38 N ATOM 1269 CA LYS A 195 −8.361 −8.603 −16.373 1.00 7.84 C ATOM 1270 C LYS A 195 −7.880 −7.209 −16.661 1.00 7.80 C ATOM 1271 O LYS A 195 −7.548 −6.881 −17.809 1.00 8.19 O ATOM 1272 CB LYS A 195 −9.878 −8.616 −16.193 1.00 8.93 C ATOM 1273 CG LYS A 195 −10.626 −8.113 −17.430 1.00 11.15 C ATOM 1274 CD LYS A 195 −12.143 −8.217 −17.274 1.00 15.05 C ATOM 1275 CE LYS A 195 −12.799 −7.627 −18.529 1.00 19.02 C ATOM 1276 NZ LYS A 195 −14.283 −7.538 −18.382 1.00 24.21 N ATOM 1277 N PHE A 196 −7.819 −6.380 −15.636 1.00 7.56 N ATOM 1278 CA PHE A 196 −7.304 −5.023 −15.758 1.00 8.40 C ATOM 1279 C PHE A 196 −5.838 −4.986 −16.216 1.00 7.99 C ATOM 1280 O PHE A 196 −5.456 −4.242 −17.150 1.00 8.27 O ATOM 1281 CB PHE A 196 −7.462 −4.291 −14.452 1.00 8.70 C ATOM 1282 CG PHE A 196 −6.824 −2.900 −14.453 1.00 9.42 C ATOM 1283 CD1 PHE A 196 −7.537 −1.806 −14.934 1.00 10.37 C ATOM 1284 CD2 PHE A 196 −5.541 −2.707 −13.985 1.00 9.93 C ATOM 1285 CE1 PHE A 196 −6.969 −0.526 −14.921 1.00 11.34 C ATOM 1286 CE2 PHE A 196 −4.980 −1.443 −14.031 1.00 11.14 C ATOM 1287 CZ PHE A 196 −5.706 −0.388 −14.498 1.00 11.40 C ATOM 1288 N ILE A 197 −5.001 −5.784 −15.597 1.00 7.59 N ATOM 1289 CA ILE A 197 −3.593 −5.775 −15.939 1.00 7.56 C ATOM 1290 C ILE A 197 −3.396 −6.199 −17.379 1.00 7.62 C ATOM 1291 O ILE A 197 −2.671 −5.550 −18.140 1.00 7.13 O ATOM 1292 CB ILE A 197 −2.773 −6.643 −14.964 1.00 8.40 C ATOM 1293 CG1 ILE A 197 −2.806 −6.020 −13.565 1.00 9.41 C ATOM 1294 CG2 ILE A 197 −1.330 −6.815 −15.474 1.00 9.06 C ATOM 1295 CD1 ILE A 197 −2.636 −6.983 −12.433 1.00 10.67 C ATOM 1296 N ARG A 198 −4.089 −7.256 −17.814 1.00 7.14 N ATOM 1297 CA ARG A 198 −4.016 −7.707 −19.190 1.00 7.82 C ATOM 1298 C ARG A 198 −4.506 −6.609 −20.142 1.00 7.51 C ATOM 1299 O ARG A 198 −3.832 −6.264 −21.114 1.00 8.14 O ATOM 1300 CB ARG A 198 −4.820 −8.994 −19.384 1.00 8.17 C ATOM 1301 CG ARG A 198 −5.227 −9.330 −20.828 1.00 8.63 C ATOM 1302 CD ARG A 198 −4.123 −9.454 −21.860 1.00 8.33 C ATOM 1303 NE ARG A 198 −3.184 −10.522 −21.577 1.00 8.47 N ATOM 1304 CZ ARG A 198 −2.123 −10.747 −22.302 1.00 8.30 C ATOM 1305 NH1 ARG A 198 −1.277 −11.723 −22.043 1.00 8.98 N ATOM 1306 NH2 ARG A 198 −1.869 −9.912 −23.329 1.00 10.05 N ATOM 1307 N ASN A 199 −5.707 −6.087 −19.900 1.00 8.40 N ATOM 1308 CA ASN A 199 −6.319 −5.175 −20.905 1.00 9.74 C ATOM 1309 C ASN A 199 −5.708 −3.814 −20.884 1.00 10.20 C ATOM 1310 O ASN A 199 −5.651 −3.181 −21.944 1.00 11.44 O ATOM 1311 CB ASN A 199 −7.813 −5.110 −20.652 1.00 11.98 C ATOM 1312 CG ASN A 199 −8.509 −6.384 −21.092 1.00 15.08 C ATOM 1313 OD1 ASN A 199 −8.024 −7.112 −21.942 1.00 20.93 O ATOM 1314 ND2 ASN A 199 −9.678 −6.591 −20.573 1.00 21.89 N ATOM 1315 N GLU A 200 −5.209 −3.342 −19.735 1.00 9.06 N ATOM 1316 CA GLU A 200 −4.754 −1.948 −19.668 1.00 9.88 C ATOM 1317 C GLU A 200 −3.255 −1.811 −19.776 1.00 9.21 C ATOM 1318 O GLU A 200 −2.761 −0.779 −20.252 1.00 9.76 O ATOM 1319 CB GLU A 200 −5.295 −1.302 −18.413 1.00 12.69 C ATOM 1320 CG GLU A 200 −6.784 −1.204 −18.369 1.00 16.37 C ATOM 1321 CD GLU A 200 −7.399 −0.633 −19.628 1.00 22.92 C ATOM 1322 OE1 GLU A 200 −6.931 0.459 −20.045 1.00 23.97 O ATOM 1323 OE2 GLU A 200 −8.380 −1.259 −20.134 1.00 30.07 O ATOM 1324 N TYR A 201 −2.503 −2.821 −19.356 1.00 8.16 N ATOM 1325 CA TYR A 201 −1.031 −2.804 −19.415 1.00 7.54 C ATOM 1326 C TYR A 201 −0.511 −3.764 −20.463 1.00 7.33 C ATOM 1327 O TYR A 201 0.253 −3.326 −21.360 1.00 7.33 O ATOM 1328 CB TYR A 201 −0.384 −3.066 −18.054 1.00 7.65 C ATOM 1329 CG TYR A 201 −0.433 −1.863 −17.174 1.00 7.88 C ATOM 1330 CD1 TYR A 201 −1.585 −1.522 −16.496 1.00 8.91 C ATOM 1331 CD2 TYR A 201 0.634 −0.944 −17.107 1.00 8.35 C ATOM 1332 CE1 TYR A 201 −1.671 −0.392 −15.719 1.00 9.75 C ATOM 1333 CE2 TYR A 201 0.538 0.217 −16.376 1.00 9.03 C ATOM 1334 CZ TYR A 201 −0.587 0.479 −15.682 1.00 9.87 C ATOM 1335 OH TYR A 201 −0.665 1.670 −14.962 1.00 13.30 O ATOM 1336 N PHE A 202 −0.842 −5.063 −20.409 1.00 7.27 N ATOM 1337 CA PHE A 202 −0.230 −5.994 −21.371 1.00 8.04 C ATOM 1338 C PHE A 202 −0.643 −5.692 −22.820 1.00 9.72 C ATOM 1339 O PHE A 202 0.196 −5.839 −23.742 1.00 13.06 O ATOM 1340 CB PHE A 202 −0.520 −7.456 −21.026 1.00 7.55 C ATOM 1341 CG PHE A 202 −0.003 −7.902 −19.681 1.00 7.31 C ATOM 1342 CD1 PHE A 202 0.879 −7.165 −18.897 1.00 7.21 C ATOM 1343 CD2 PHE A 202 −0.367 −9.138 −19.242 1.00 7.49 C ATOM 1344 CE1 PHE A 202 1.385 −7.663 −17.729 1.00 7.77 C ATOM 1345 CE2 PHE A 202 0.160 −9.664 −18.086 1.00 8.05 C ATOM 1346 CZ PHE A 202 1.028 −8.921 −17.314 1.00 8.15 C ATOM 1347 N GLU A 203 −1.853 −5.229 −23.014 1.00 9.34 N ATOM 1348 CA GLU A 203 −2.343 −4.853 −24.345 1.00 10.34 C ATOM 1349 C GLU A 203 −1.862 −3.490 −24.744 1.00 11.30 C ATOM 1350 O GLU A 203 −2.124 −3.060 −25.894 1.00 13.90 O ATOM 1351 CB GLU A 203 −3.863 −4.952 −24.402 1.00 11.29 C ATOM 1352 CG GLU A 203 −4.381 −6.375 −24.313 1.00 12.97 C ATOM 1353 CD GLU A 203 −3.911 −7.242 −25.404 1.00 15.87 C ATOM 1354 OE1 GLU A 203 −4.053 −6.884 −26.619 1.00 19.60 O ATOM 1355 OE2 GLU A 203 −3.372 −8.316 −25.139 1.00 15.76 O ATOM 1356 N ASN A 204 −1.151 −2.777 −23.864 1.00 9.41 N ATOM 1357 CA ASN A 204 −0.512 −1.452 −24.125 1.00 9.73 C ATOM 1358 C ASN A 204 1.013 −1.604 −24.290 1.00 9.49 C ATOM 1359 O ASN A 204 1.734 −0.607 −24.096 1.00 10.48 O ATOM 1360 CB ASN A 204 −0.843 −0.454 −23.031 1.00 10.24 C ATOM 1361 CG ASN A 204 −0.482 0.981 −23.392 1.00 11.41 C ATOM 1362 OD1 ASN A 204 −0.868 1.439 −24.502 1.00 14.31 O ATOM 1363 ND2 ASN A 204 0.207 1.665 −22.522 1.00 11.45 N ATOM 1364 N ASN A 205 1.497 −2.824 −24.544 1.00 9.76 N ATOM 1365 CA ASN A 205 2.925 −3.046 −24.746 1.00 10.66 C ATOM 1366 C ASN A 205 3.763 −2.755 −23.497 1.00 10.24 C ATOM 1367 O ASN A 205 4.948 −2.480 −23.580 1.00 11.65 O ATOM 1368 CB ASN A 205 3.461 −2.259 −25.978 1.00 12.47 C ATOM 1369 CG ASN A 205 4.835 −2.740 −26.423 1.00 16.50 C ATOM 1370 OD1 ASN A 205 5.072 −3.968 −26.457 1.00 19.03 O ATOM 1371 ND2 ASN A 205 5.755 −1.802 −26.795 1.00 19.61 N ATOM 1372 N GLN A 206 3.153 −2.906 −22.321 1.00 7.91 N ATOM 1373 CA GLN A 206 3.870 −2.845 −21.052 1.00 7.51 C ATOM 1374 C GLN A 206 3.845 −4.270 −20.505 1.00 7.69 C ATOM 1375 O GLN A 206 2.871 −4.666 −19.862 1.00 8.24 O ATOM 1376 CB GLN A 206 3.223 −1.821 −20.121 1.00 7.54 C ATOM 1377 CG GLN A 206 3.407 −0.379 −20.586 1.00 7.50 C ATOM 1378 CD GLN A 206 2.625 0.605 −19.762 1.00 8.05 C ATOM 1379 OE1 GLN A 206 1.443 0.851 −20.054 1.00 9.06 O ATOM 1380 NE2 GLN A 206 3.234 1.157 −18.740 1.00 7.49 N ATOM 1381 N LEU A 207 4.874 −5.021 −20.865 1.00 7.12 N ATOM 1382 CA LEU A 207 4.895 −6.485 −20.783 1.00 8.15 C ATOM 1383 C LEU A 207 5.728 −7.007 −19.638 1.00 7.25 C ATOM 1384 O LEU A 207 5.741 −8.237 −19.425 1.00 8.07 O ATOM 1385 CB LEU A 207 5.421 −7.114 −22.101 1.00 9.90 C ATOM 1386 CG LEU A 207 4.910 −6.545 −23.400 1.00 11.10 C ATOM 1387 CD1 LEU A 207 5.569 −7.308 −24.583 1.00 11.81 C ATOM 1388 CD2 LEU A 207 3.392 −6.687 −23.462 1.00 10.71 C ATOM 1389 N ASN A 208 6.440 −6.144 −18.941 1.00 7.13 N ATOM 1390 CA ASN A 208 7.243 −6.544 −17.785 1.00 6.60 C ATOM 1391 C ASN A 208 7.129 −5.470 −16.756 1.00 6.65 C ATOM 1392 O ASN A 208 6.586 −4.381 −16.972 1.00 6.08 O ATOM 1393 CB ASN A 208 8.668 −6.907 −18.232 1.00 6.89 C ATOM 1394 CG ASN A 208 9.432 −5.740 −18.792 1.00 6.61 C ATOM 1395 OD1 ASN A 208 9.307 −4.583 −18.351 1.00 7.50 O ATOM 1396 ND2 ASN A 208 10.329 −6.041 −19.755 1.00 7.61 N ATOM 1397 N LEU A 209 7.665 −5.763 −15.570 1.00 6.24 N ATOM 1398 CA LEU A 209 7.502 −4.853 −14.441 1.00 6.28 C ATOM 1399 C LEU A 209 8.168 −3.492 −14.728 1.00 5.86 C ATOM 1400 O LEU A 209 7.677 −2.429 −14.382 1.00 6.04 O ATOM 1401 CB LEU A 209 8.099 −5.433 −13.169 1.00 6.65 C ATOM 1402 CG LEU A 209 7.354 −6.672 −12.635 1.00 7.16 C ATOM 1403 CD1 LEU A 209 8.175 −7.306 −11.528 1.00 8.03 C ATOM 1404 CD2 LEU A 209 6.007 −6.318 −12.145 1.00 7.74 C ATOM 1405 N TYR A 210 9.344 −3.531 −15.342 1.00 6.21 N ATOM 1406 CA TYR A 210 10.048 −2.286 −15.690 1.00 6.57 C ATOM 1407 C TYR A 210 9.153 −1.389 −16.565 1.00 6.35 C ATOM 1408 O TYR A 210 9.074 −0.189 −16.317 1.00 6.65 O ATOM 1409 CB TYR A 210 11.400 −2.580 −16.385 1.00 7.03 C ATOM 1410 CG TYR A 210 12.190 −1.307 −16.597 1.00 7.47 C ATOM 1411 CD1 TYR A 210 12.972 −0.751 −15.581 1.00 7.34 C ATOM 1412 CD2 TYR A 210 12.124 −0.616 −17.796 1.00 8.27 C ATOM 1413 CE1 TYR A 210 13.664 0.441 −15.749 1.00 7.88 C ATOM 1414 CE2 TYR A 210 12.803 0.569 −17.952 1.00 7.75 C ATOM 1415 CZ TYR A 210 13.572 1.100 −16.950 1.00 8.52 C ATOM 1416 OH TYR A 210 14.248 2.307 −17.094 1.00 9.62 O ATOM 1417 N GLN A 211 8.540 −1.953 −17.596 1.00 6.46 N ATOM 1418 CA GLN A 211 7.713 −1.162 −18.485 1.00 6.33 C ATOM 1419 C GLN A 211 6.470 −0.683 −17.786 1.00 6.37 C ATOM 1420 O GLN A 211 5.965 0.398 −18.056 1.00 6.53 O ATOM 1421 CB GLN A 211 7.365 −1.957 −19.738 1.00 7.08 C ATOM 1422 CG GLN A 211 8.524 −2.240 −20.679 1.00 7.78 C ATOM 1423 CD GLN A 211 8.123 −3.147 −21.791 1.00 10.07 C ATOM 1424 OE1 GLN A 211 7.515 −4.194 −21.575 1.00 10.30 O ATOM 1425 NE2 GLN A 211 8.525 −2.777 −23.014 1.00 12.59 N ATOM 1426 N MET A 212 5.879 −1.529 −16.934 1.00 6.50 N ATOM 1427 CA MET A 212 4.710 −1.113 −16.151 1.00 6.34 C ATOM 1428 C MET A 212 5.012 0.099 −15.272 1.00 6.21 C ATOM 1429 O MET A 212 4.163 0.983 −15.067 1.00 6.78 O ATOM 1430 CB MET A 212 4.190 −2.274 −15.297 1.00 6.69 C ATOM 1431 CG MET A 212 3.580 −3.385 −16.138 1.00 6.84 C ATOM 1432 SD MET A 212 3.061 −4.836 −15.151 1.00 7.41 S ATOM 1433 CE MET A 212 1.616 −4.207 −14.303 1.00 8.80 C ATOM 1434 N ARG A 213 6.213 0.098 −14.685 1.00 5.84 N ATOM 1435 CA ARG A 213 6.594 1.145 −13.760 1.00 6.51 C ATOM 1436 C ARG A 213 7.132 2.416 −14.422 1.00 6.39 C ATOM 1437 O ARG A 213 6.873 3.506 −13.930 1.00 6.80 O ATOM 1438 CB ARG A 213 7.643 0.606 −12.754 1.00 6.88 C ATOM 1439 CG ARG A 213 8.148 1.657 −11.792 1.00 7.26 C ATOM 1440 CD ARG A 213 7.059 2.240 −10.916 1.00 7.79 C ATOM 1441 NE ARG A 213 7.549 3.256 −10.000 1.00 8.27 N ATOM 1442 CZ ARG A 213 7.809 4.524 −10.323 1.00 8.92 C ATOM 1443 NH1 ARG A 213 7.656 4.981 −11.575 1.00 9.05 N ATOM 1444 NH2 ARG A 213 8.222 5.373 −9.384 1.00 10.03 N ATOM 1445 N TRP A 214 7.899 2.260 −15.503 1.00 6.03 N ATOM 1446 CA TRP A 214 8.654 3.382 −16.085 1.00 6.29 C ATOM 1447 C TRP A 214 8.244 3.716 −17.503 1.00 6.56 C ATOM 1448 O TRP A 214 8.839 4.651 −18.091 1.00 6.89 O ATOM 1449 CB TRP A 214 10.170 3.130 −15.986 1.00 6.80 C ATOM 1450 CG TRP A 214 10.654 2.956 −14.578 1.00 7.02 C ATOM 1451 CD1 TRP A 214 11.071 1.806 −14.025 1.00 8.23 C ATOM 1452 CD2 TRP A 214 10.810 3.981 −13.582 1.00 7.33 C ATOM 1453 NE1 TRP A 214 11.473 2.053 −12.757 1.00 8.29 N ATOM 1454 CE2 TRP A 214 11.294 3.362 −12.429 1.00 7.98 C ATOM 1455 CE3 TRP A 214 10.558 5.357 −13.549 1.00 7.76 C ATOM 1456 CZ2 TRP A 214 11.557 4.080 −11.244 1.00 8.94 C ATOM 1457 CZ3 TRP A 214 10.841 6.067 −12.390 1.00 8.61 C ATOM 1458 CH2 TRP A 214 11.310 5.400 −11.239 1.00 9.27 C ATOM 1459 N ASN A 215 7.340 2.954 −18.092 1.00 6.77 N ATOM 1460 CA ASN A 215 6.826 3.144 −19.466 1.00 7.03 C ATOM 1461 C ASN A 215 7.813 3.842 −20.383 1.00 7.20 C ATOM 1462 O ASN A 215 7.554 4.966 −20.844 1.00 7.12 O ATOM 1463 CB ASN A 215 5.505 3.899 −19.418 1.00 7.25 C ATOM 1464 CG ASN A 215 4.682 3.759 −20.672 1.00 7.99 C ATOM 1465 OD1 ASN A 215 4.991 2.955 −21.540 1.00 9.17 O ATOM 1466 ND2 ASN A 215 3.600 4.470 −20.739 1.00 8.48 N ATOM 1467 N PRO A 216 8.930 3.231 −20.698 1.00 7.26 N ATOM 1468 CA PRO A 216 9.944 3.947 −21.468 1.00 7.94 C ATOM 1469 C PRO A 216 9.499 4.349 −22.852 1.00 7.81 C ATOM 1470 O PRO A 216 10.085 5.305 −23.448 1.00 8.19 O ATOM 1471 CB PRO A 216 11.108 2.944 −21.563 1.00 8.36 C ATOM 1472 CG PRO A 216 10.539 1.658 −21.075 1.00 10.18 C ATOM 1473 CD PRO A 216 9.404 1.940 −20.188 1.00 8.06 C ATOM 1474 N GLU A 217 8.492 3.700 −23.409 1.00 7.41 N ATOM 1475 CA GLU A 217 7.928 4.105 −24.726 1.00 8.39 C ATOM 1476 C GLU A 217 7.270 5.466 −24.630 1.00 8.27 C ATOM 1477 O GLU A 217 7.323 6.266 −25.595 1.00 9.09 O ATOM 1478 CB GLU A 217 6.925 3.087 −25.215 1.00 9.46 C ATOM 1479 CG GLU A 217 6.417 3.250 −26.635 1.00 11.15 C ATOM 1480 CD GLU A 217 5.548 2.097 −27.087 1.00 14.15 C ATOM 1481 OE1 GLU A 217 5.197 1.160 −26.328 1.00 16.32 O ATOM 1482 OE2 GLU A 217 5.267 2.061 −28.295 1.00 15.75 O ATOM 1483 N ASN A 218 6.673 5.808 −23.481 1.00 7.35 N ATOM 1484 CA ASN A 218 6.025 7.086 −23.205 1.00 7.25 C ATOM 1485 C ASN A 218 6.260 7.487 −21.736 1.00 6.73 C ATOM 1486 O ASN A 218 5.373 7.358 −20.874 1.00 6.63 O ATOM 1487 CB ASN A 218 4.515 7.104 −23.525 1.00 7.13 C ATOM 1488 CG ASN A 218 4.237 7.015 −24.997 1.00 7.78 C ATOM 1489 OD1 ASN A 218 3.919 5.911 −25.518 1.00 11.30 O ATOM 1490 ND2 ASN A 218 4.370 8.069 −25.662 1.00 6.47 N ATOM 1491 N PRO A 219 7.481 7.965 −21.449 1.00 6.75 N ATOM 1492 CA PRO A 219 7.878 8.140 −20.048 1.00 7.10 C ATOM 1493 C PRO A 219 6.941 9.049 −19.262 1.00 7.33 C ATOM 1494 O PRO A 219 6.491 10.114 −19.748 1.00 7.68 O ATOM 1495 CB PRO A 219 9.272 8.756 −20.168 1.00 6.88 C ATOM 1496 CG PRO A 219 9.799 8.227 −21.446 1.00 7.12 C ATOM 1497 CD PRO A 219 8.601 8.210 −22.367 1.00 6.98 C ATOM 1498 N ALA A 220 6.672 8.667 −18.019 1.00 7.60 N ATOM 1499 CA ALA A 220 5.918 9.400 −17.033 1.00 9.04 C ATOM 1500 C ALA A 220 4.424 9.292 −17.210 1.00 10.08 C ATOM 1501 O ALA A 220 3.679 9.852 −16.374 1.00 15.33 O ATOM 1502 CB ALA A 220 6.434 10.824 −16.848 1.00 9.71 C ATOM 1503 N GLN A 221 3.950 8.641 −18.253 1.00 8.06 N ATOM 1504 CA GLN A 221 2.545 8.401 −18.480 1.00 8.88 C ATOM 1505 C GLN A 221 2.226 6.954 −18.213 1.00 8.23 C ATOM 1506 O GLN A 221 3.053 6.077 −18.467 1.00 8.67 O ATOM 1507 CB GLN A 221 2.143 8.659 −19.970 1.00 10.96 C ATOM 1508 CG GLN A 221 2.264 10.115 −20.323 1.00 13.36 C ATOM 1509 CD GLN A 221 1.601 10.391 −21.628 1.00 15.17 C ATOM 1510 OE1 GLN A 221 2.227 10.475 −22.696 1.00 14.40 O ATOM 1511 NE2 GLN A 221 0.319 10.467 −21.561 1.00 17.70 N ATOM 1512 N HIS A 222 1.005 6.682 −17.749 1.00 8.27 N ATOM 1513 CA HIS A 222 0.489 5.336 −17.661 1.00 8.86 C ATOM 1514 C HIS A 222 1.453 4.399 −16.921 1.00 7.81 C ATOM 1515 O HIS A 222 1.762 3.289 −17.383 1.00 8.43 O ATOM 1516 CB HIS A 222 0.047 4.818 −19.005 1.00 10.19 C ATOM 1517 CG HIS A 222 −0.858 3.663 −18.899 1.00 12.71 C ATOM 1518 ND1 HIS A 222 −2.204 3.748 −18.577 1.00 16.88 N ATOM 1519 CD2 HIS A 222 −0.574 2.353 −18.973 1.00 10.87 C ATOM 1520 CE1 HIS A 222 −2.701 2.514 −18.510 1.00 17.21 C ATOM 1521 NE2 HIS A 222 −1.730 1.662 −18.815 1.00 16.74 N ATOM 1522 N GLN A 223 1.873 4.833 −15.750 1.00 7.61 N ATOM 1523 CA GLN A 223 2.780 4.083 −14.857 1.00 7.70 C ATOM 1524 C GLN A 223 2.024 3.565 −13.676 1.00 7.72 C ATOM 1525 O GLN A 223 1.129 4.187 −13.147 1.00 8.85 O ATOM 1526 CB GLN A 223 4.009 4.913 −14.476 1.00 7.61 C ATOM 1527 CG GLN A 223 4.867 5.209 −15.700 1.00 7.38 C ATOM 1528 CD GLN A 223 6.054 6.111 −15.542 1.00 7.38 C ATOM 1529 OE1 GLN A 223 6.804 6.208 −16.522 1.00 7.62 O ATOM 1530 NE2 GLN A 223 6.254 6.762 −14.395 1.00 8.38 N ATOM 1531 N TYR A 224 2.422 2.359 −13.235 1.00 7.71 N ATOM 1532 CA TYR A 224 1.546 1.627 −12.280 1.00 8.87 C ATOM 1533 C TYR A 224 1.579 2.240 −10.910 1.00 9.34 C ATOM 1534 O TYR A 224 0.558 2.143 −10.189 1.00 11.57 O ATOM 1535 CB TYR A 224 1.987 0.120 −12.222 1.00 8.94 C ATOM 1536 CG TYR A 224 0.826 −0.753 −11.797 1.00 8.89 C ATOM 1537 CD1 TYR A 224 0.533 −0.891 −10.445 1.00 9.85 C ATOM 1538 CD2 TYR A 224 0.031 −1.347 −12.719 1.00 8.86 C ATOM 1539 CE1 TYR A 224 −0.601 −1.619 −10.054 1.00 9.71 C ATOM 1540 CE2 TYR A 224 −1.108 −2.077 −12.320 1.00 10.55 C ATOM 1541 CZ TYR A 224 −1.355 −2.189 −10.987 1.00 9.87 C ATOM 1542 OH TYR A 224 −2.506 −2.958 −10.641 1.00 11.85 O ATOM 1543 N ALA A 225 2.711 2.785 −10.478 1.00 9.37 N ATOM 1544 CA ALA A 225 2.915 3.186 −9.095 1.00 11.00 C ATOM 1545 C ALA A 225 3.896 4.311 −8.986 1.00 11.56 C ATOM 1546 O ALA A 225 4.747 4.497 −9.864 1.00 11.95 O ATOM 1547 CB ALA A 225 3.421 1.998 −8.288 1.00 12.74 C ATOM 1548 N SER A 226 3.854 5.005 −7.839 1.00 11.98 N ATOM 1549 CA ASER A 226 4.812 6.043 −7.495 0.60 12.16 C ATOM 1550 CA BSER A 226 4.812 6.048 −7.510 0.40 11.85 C ATOM 1551 C SER A 226 6.053 5.551 −6.778 1.00 11.20 C ATOM 1552 O SER A 226 7.101 6.177 −6.860 1.00 10.45 O ATOM 1553 CB ASER A 226 4.150 7.134 −6.652 0.60 13.31 C ATOM 1554 CB BSER A 226 4.140 7.165 −6.707 0.40 12.48 C ATOM 1555 OG ASER A 226 3.179 7.842 −7.392 0.60 15.79 O ATOM 1556 OG BSER A 226 3.893 6.778 −5.364 0.40 13.70 O ATOM 1557 N ASP A 227 5.940 4.400 −6.060 1.00 10.94 N ATOM 1558 CA ASP A 227 7.065 3.852 −5.330 1.00 10.68 C ATOM 1559 C ASP A 227 8.171 3.514 −6.328 1.00 9.88 C ATOM 1560 O ASP A 227 7.972 2.665 −7.207 1.00 9.07 O ATOM 1561 CB ASP A 227 6.642 2.586 −4.569 1.00 12.10 C ATOM 1562 CG ASP A 227 7.748 1.938 −3.817 1.00 11.76 C ATOM 1563 OD1 ASP A 227 8.886 2.347 −3.775 1.00 12.89 O ATOM 1564 OD2 ASP A 227 7.343 0.876 −3.213 1.00 15.20 O ATOM 1565 N ILE A 228 9.327 4.144 −6.220 1.00 9.30 N ATOM 1566 CA ILE A 228 10.459 3.886 −7.080 1.00 10.18 C ATOM 1567 C ILE A 228 11.009 2.441 −7.015 1.00 9.78 C ATOM 1568 O ILE A 228 11.754 2.031 −7.897 1.00 10.41 O ATOM 1569 CB ILE A 228 11.573 4.921 −6.923 1.00 11.26 C ATOM 1570 CG1 ILE A 228 12.306 4.808 −5.589 1.00 12.58 C ATOM 1571 CG2 ILE A 228 11.082 6.319 −7.198 1.00 12.04 C ATOM 1572 CD1 ILE A 228 13.431 5.813 −5.439 1.00 14.00 C ATOM 1573 N ARG A 229 10.676 1.733 −5.915 1.00 10.12 N ATOM 1574 CA AARG A 229 11.065 0.306 −5.829 0.60 11.12 C ATOM 1575 CA BARG A 229 11.003 0.322 −5.696 0.40 10.78 C ATOM 1576 C ARG A 229 9.875 −0.650 −5.949 1.00 9.73 C ATOM 1577 O ARG A 229 10.000 −1.845 −5.642 1.00 9.48 O ATOM 1578 CB AARG A 229 11.940 0.010 −4.581 0.60 12.50 C ATOM 1579 CB BARG A 229 11.430 0.132 −4.249 0.40 11.71 C ATOM 1580 CG AARG A 229 13.316 0.696 −4.638 0.60 14.75 C ATOM 1581 CG BARG A 229 12.610 1.007 −3.892 0.40 13.52 C ATOM 1582 CD AARG A 229 14.209 0.332 −3.470 0.60 18.05 C ATOM 1583 CD BARG A 229 13.324 0.424 −2.698 0.40 16.03 C ATOM 1584 NE AARG A 229 14.564 −1.080 −3.498 0.60 21.14 N ATOM 1585 NE BARG A 229 14.046 −0.809 −3.008 0.40 18.32 N ATOM 1586 CZ AARG A 229 15.523 −1.633 −4.224 0.60 23.81 C ATOM 1587 CZ BARG A 229 13.712 −2.031 −2.586 0.40 18.64 C ATOM 1588 NH1 AARG A 229 16.303 −0.919 −5.031 0.60 26.97 N ATOM 1589 NH1 BARG A 229 12.641 −2.238 −1.839 0.40 18.97 N ATOM 1590 NH2 AARG A 229 15.697 −2.927 −4.135 0.60 24.59 N ATOM 1591 NH2 BARG A 229 14.464 −3.064 −2.945 0.40 21.03 N ATOM 1592 N TRP A 230 8.766 −0.187 −6.538 1.00 8.52 N ATOM 1593 CA TRP A 230 7.610 −1.056 −6.796 1.00 8.16 C ATOM 1594 C TRP A 230 7.992 −2.324 −7.548 1.00 8.72 C ATOM 1595 O TRP A 230 7.565 −3.435 −7.195 1.00 9.72 O ATOM 1596 CB TRP A 230 6.556 −0.294 −7.615 1.00 8.38 C ATOM 1597 CG TRP A 230 5.372 −1.046 −8.043 1.00 8.01 C ATOM 1598 CD1 TRP A 230 4.190 −1.245 −7.322 1.00 8.11 C ATOM 1599 CD2 TRP A 230 5.138 −1.691 −9.319 1.00 7.44 C ATOM 1600 NE1 TRP A 230 3.281 −1.926 −8.092 1.00 8.31 N ATOM 1601 CE2 TRP A 230 3.834 −2.200 −9.313 1.00 7.84 C ATOM 1602 CE3 TRP A 230 5.906 −1.862 −10.459 1.00 7.69 C ATOM 1603 CZ2 TRP A 230 3.329 −2.944 −10.378 1.00 7.46 C ATOM 1604 CZ3 TRP A 230 5.401 −2.525 −11.500 1.00 7.49 C ATOM 1605 CH2 TRP A 230 4.136 −3.069 −11.478 1.00 7.36 C ATOM 1606 N ALA A 231 8.764 −2.192 −8.620 1.00 7.66 N ATOM 1607 CA ALA A 231 9.141 −3.354 −9.434 1.00 8.11 C ATOM 1608 C ALA A 231 10.044 −4.262 −8.677 1.00 8.88 C ATOM 1609 O ALA A 231 9.938 −5.490 −8.779 1.00 9.79 O ATOM 1610 CB ALA A 231 9.771 −2.897 −10.740 1.00 8.21 C ATOM 1611 N ASP A 232 10.990 −3.681 −7.939 1.00 8.54 N ATOM 1612 CA ASP A 232 11.935 −4.510 −7.176 1.00 9.45 C ATOM 1613 C ASP A 232 11.229 −5.376 −6.157 1.00 8.67 C ATOM 1614 O ASP A 232 11.623 −6.536 −5.959 1.00 9.74 O ATOM 1615 CB ASP A 232 12.973 −3.600 −6.448 1.00 11.74 C ATOM 1616 CG ASP A 232 13.720 −2.756 −7.407 1.00 15.12 C ATOM 1617 OD1 ASP A 232 14.613 −3.300 −8.085 1.00 19.62 O ATOM 1618 OD2 ASP A 232 13.313 −1.615 −7.665 1.00 19.29 O ATOM 1619 N LYS A 233 10.225 −4.857 −5.468 1.00 8.57 N ATOM 1620 CA LYS A 233 9.534 −5.616 −4.409 1.00 8.87 C ATOM 1621 C LYS A 233 8.838 −6.821 −5.014 1.00 8.92 C ATOM 1622 O LYS A 233 8.910 −7.939 −4.475 1.00 9.50 O ATOM 1623 CB LYS A 233 8.571 −4.711 −3.669 1.00 10.21 C ATOM 1624 CG LYS A 233 9.275 −3.678 −2.816 1.00 12.00 C ATOM 1625 CD LYS A 233 8.336 −2.593 −2.337 1.00 15.08 C ATOM 1626 CE LYS A 233 9.112 −1.588 −1.506 1.00 19.08 C ATOM 1627 NZ LYS A 233 8.190 −0.536 −1.019 1.00 22.77 N ATOM 1628 N ILE A 234 8.156 −6.613 −6.130 1.00 8.10 N ATOM 1629 CA ILE A 234 7.401 −7.707 −6.738 1.00 7.35 C ATOM 1630 C ILE A 234 8.386 −8.723 −7.308 1.00 6.93 C ATOM 1631 O ILE A 234 8.216 −9.942 −7.183 1.00 6.78 O ATOM 1632 CB ILE A 234 6.424 −7.140 −7.804 1.00 6.89 C ATOM 1633 CG1 ILE A 234 5.382 −6.240 −7.147 1.00 7.52 C ATOM 1634 CG2 ILE A 234 5.781 −8.307 −8.554 1.00 6.94 C ATOM 1635 CD1 ILE A 234 4.578 −5.443 −8.156 1.00 8.31 C ATOM 1636 N ALA A 235 9.426 −8.257 −7.997 1.00 7.53 N ATOM 1637 CA ALA A 235 10.448 −9.124 −8.634 1.00 7.91 C ATOM 1638 C ALA A 235 11.141 −10.002 −7.595 1.00 8.47 C ATOM 1639 O ALA A 235 11.474 −11.167 −7.888 1.00 8.24 O ATOM 1640 CB ALA A 235 11.476 −8.270 −9.374 1.00 8.69 C ATOM 1641 N LYS A 236 11.386 −9.482 −6.386 1.00 8.78 N ATOM 1642 CA LYS A 236 12.112 −10.275 −5.365 1.00 9.92 C ATOM 1643 C LYS A 236 11.247 −11.451 −4.979 1.00 9.35 C ATOM 1644 O LYS A 236 11.726 −12.584 −4.862 1.00 9.53 O ATOM 1645 CB LYS A 236 12.373 −9.409 −4.148 1.00 12.90 C ATOM 1646 CG LYS A 236 13.179 −10.173 −3.100 1.00 17.22 C ATOM 1647 CD LYS A 236 13.573 −9.300 −1.920 1.00 22.63 C ATOM 1648 CE LYS A 236 12.447 −9.057 −0.948 1.00 28.29 C ATOM 1649 NZ LYS A 236 11.702 −10.247 −0.503 1.00 33.02 N ATOM 1650 N LEU A 237 9.949 −11.266 −4.824 1.00 8.51 N ATOM 1651 CA LEU A 237 9.077 −12.382 −4.474 1.00 9.46 C ATOM 1652 C LEU A 237 8.975 −13.326 −5.616 1.00 8.68 C ATOM 1653 O LEU A 237 8.962 −14.558 −5.475 1.00 8.63 O ATOM 1654 CB LEU A 237 7.655 −11.966 −4.082 1.00 11.04 C ATOM 1655 CG LEU A 237 7.482 −11.104 −2.844 1.00 13.90 C ATOM 1656 CD1 LEU A 237 5.992 −10.987 −2.492 1.00 14.91 C ATOM 1657 CD2 LEU A 237 8.279 −11.620 −1.666 1.00 16.69 C ATOM 1658 N MET A 238 8.869 −12.821 −6.844 1.00 7.60 N ATOM 1659 CA MET A 238 8.805 −13.687 −8.005 1.00 8.38 C ATOM 1660 C MET A 238 10.024 −14.546 −8.113 1.00 7.72 C ATOM 1661 O MET A 238 9.909 −15.761 −8.401 1.00 7.58 O ATOM 1662 CB MET A 238 8.704 −12.881 −9.288 1.00 8.37 C ATOM 1663 CG MET A 238 7.339 −12.239 −9.473 1.00 8.34 C ATOM 1664 SD MET A 238 7.287 −11.237 −10.996 1.00 10.44 S ATOM 1665 CE MET A 238 7.393 −12.539 −12.130 1.00 14.22 C ATOM 1666 N ASP A 239 11.198 −13.983 −7.869 1.00 7.85 N ATOM 1667 CA ASP A 239 12.439 −14.783 −8.001 1.00 8.35 C ATOM 1668 C ASP A 239 12.469 −15.885 −6.951 1.00 7.52 C ATOM 1669 O ASP A 239 12.838 −17.024 −7.289 1.00 7.90 O ATOM 1670 CB ASP A 239 13.669 −13.884 −7.854 1.00 9.82 C ATOM 1671 CG ASP A 239 14.876 −14.404 −8.622 1.00 13.02 C ATOM 1672 OD1 ASP A 239 14.712 −15.192 −9.557 1.00 16.65 O ATOM 1673 OD2 ASP A 239 15.969 −13.902 −8.302 1.00 17.18 O ATOM 1674 N LYS A 240 12.033 −15.597 −5.744 1.00 7.34 N ATOM 1675 CA LYS A 240 11.981 −16.661 −4.727 1.00 8.07 C ATOM 1676 C LYS A 240 11.058 −17.756 −5.187 1.00 8.58 C ATOM 1677 O LYS A 240 11.353 −18.961 −5.061 1.00 8.95 O ATOM 1678 CB LYS A 240 11.537 −16.130 −3.426 1.00 9.33 C ATOM 1679 CG LYS A 240 12.580 −15.267 −2.749 1.00 10.99 C ATOM 1680 CD LYS A 240 12.082 −14.633 −1.458 1.00 12.12 C ATOM 1681 CE LYS A 240 12.169 −15.592 −0.326 1.00 12.89 C ATOM 1682 NZ LYS A 240 11.846 −14.852 0.960 1.00 14.11 N ATOM 1683 N SER A 241 9.868 −17.407 −5.747 1.00 7.98 N ATOM 1684 CA SER A 241 8.932 −18.453 −6.144 1.00 8.66 C ATOM 1685 C SER A 241 9.430 −19.260 −7.333 1.00 8.98 C ATOM 1686 O SER A 241 9.252 −20.484 −7.380 1.00 8.80 O ATOM 1687 CB SER A 241 7.580 −17.793 −6.473 1.00 9.98 C ATOM 1688 OG SER A 241 7.041 −17.193 −5.335 1.00 11.45 O ATOM 1689 N TYR A 242 10.086 −18.590 −8.281 1.00 8.22 N ATOM 1690 CA TYR A 242 10.721 −19.278 −9.375 1.00 8.41 C ATOM 1691 C TYR A 242 11.710 −20.316 −8.876 1.00 9.28 C ATOM 1692 O TYR A 242 11.760 −21.434 −9.403 1.00 10.26 O ATOM 1693 CB TYR A 242 11.385 −18.287 −10.385 1.00 8.81 C ATOM 1694 CG TYR A 242 10.451 −17.876 −11.491 1.00 8.19 C ATOM 1695 CD1 TYR A 242 9.283 −17.160 −11.223 1.00 8.80 C ATOM 1696 CD2 TYR A 242 10.732 −18.178 −12.802 1.00 9.42 C ATOM 1697 CE1 TYR A 242 8.414 −16.815 −12.241 1.00 9.71 C ATOM 1698 CE2 TYR A 242 9.836 −17.800 −13.837 1.00 9.77 C ATOM 1699 CZ TYR A 242 8.681 −17.125 −13.508 1.00 9.10 C ATOM 1700 OH TYR A 242 7.793 −16.794 −14.506 1.00 8.88 O ATOM 1701 N LYS A 243 12.563 −19.922 −7.949 1.00 8.60 N ATOM 1702 CA LYS A 243 13.602 −20.842 −7.458 1.00 9.19 C ATOM 1703 C LYS A 243 13.045 −21.906 −6.538 1.00 10.00 C ATOM 1704 O LYS A 243 13.614 −23.027 −6.448 1.00 13.52 O ATOM 1705 CB LYS A 243 14.721 −20.023 −6.812 1.00 8.88 C ATOM 1706 CG LYS A 243 15.485 −19.201 −7.816 1.00 9.25 C ATOM 1707 CD LYS A 243 16.616 −18.349 −7.257 1.00 10.46 C ATOM 1708 CE LYS A 243 17.248 −17.582 −8.402 1.00 11.85 C ATOM 1709 NZ LYS A 243 18.409 −16.784 −7.926 1.00 12.92 N ATOM 1710 N GLN A 244 11.922 −21.660 −5.918 1.00 8.82 N ATOM 1711 CA GLN A 244 11.231 −22.631 −5.056 1.00 9.11 C ATOM 1712 C GLN A 244 10.677 −23.760 −5.924 1.00 9.92 C ATOM 1713 O GLN A 244 10.775 −24.966 −5.554 1.00 11.13 O ATOM 1714 CB GLN A 244 10.146 −21.979 −4.194 1.00 9.73 C ATOM 1715 CG GLN A 244 9.338 −22.967 −3.373 1.00 9.77 C ATOM 1716 CD GLN A 244 8.454 −22.300 −2.385 1.00 11.38 C ATOM 1717 OE1 GLN A 244 8.638 −21.131 −2.090 1.00 14.86 O ATOM 1718 NE2 GLN A 244 7.586 −23.042 −1.801 1.00 13.79 N ATOM 1719 N PHE A 245 10.091 −23.455 −7.091 1.00 9.57 N ATOM 1720 CA PHE A 245 9.416 −24.468 −7.919 1.00 11.08 C ATOM 1721 C PHE A 245 10.197 −24.842 −9.151 1.00 12.49 C ATOM 1722 O PHE A 245 9.755 −25.723 −9.877 1.00 14.14 O ATOM 1723 CB PHE A 245 8.017 −23.934 −8.232 1.00 10.85 C ATOM 1724 CG PHE A 245 7.140 −23.825 −7.033 1.00 10.74 C ATOM 1725 CD1 PHE A 245 6.576 −24.977 −6.467 1.00 11.73 C ATOM 1726 CD2 PHE A 245 6.924 −22.615 −6.391 1.00 12.01 C ATOM 1727 CE1 PHE A 245 5.791 −24.893 −5.349 1.00 11.61 C ATOM 1728 CE2 PHE A 245 6.138 −22.552 −5.250 1.00 12.24 C ATOM 1729 CZ PHE A 245 5.585 −23.708 −4.747 1.00 11.82 C ATOM 1730 N GLY A 246 11.321 −24.197 −9.432 1.00 10.78 N ATOM 1731 CA GLY A 246 12.136 −24.577 −10.566 1.00 12.04 C ATOM 1732 C GLY A 246 11.529 −24.131 −11.882 1.00 12.28 C ATOM 1733 O GLY A 246 11.546 −24.883 −12.884 1.00 14.60 O ATOM 1734 N ILE A 247 10.985 −22.920 −11.924 1.00 11.02 N ATOM 1735 CA ILE A 247 10.268 −22.440 −13.119 1.00 11.61 C ATOM 1736 C ILE A 247 11.253 −21.769 −14.076 1.00 11.15 C ATOM 1737 O ILE A 247 12.141 −21.036 −13.629 1.00 12.49 O ATOM 1738 CB ILE A 247 9.161 −21.448 −12.671 1.00 11.24 C ATOM 1739 CG1 ILE A 247 8.194 −22.036 −11.600 1.00 12.62 C ATOM 1740 CG2 ILE A 247 8.339 −20.946 −13.850 1.00 12.14 C ATOM 1741 CD1 ILE A 247 7.406 −23.237 −12.039 1.00 13.37 C ATOM 1742 N LYS A 248 11.064 −22.020 −15.373 1.00 12.90 N ATOM 1743 CA LYS A 248 11.883 −21.455 −16.417 1.00 14.30 C ATOM 1744 C LYS A 248 11.404 −20.014 −16.705 1.00 12.81 C ATOM 1745 O LYS A 248 10.205 −19.791 −16.912 1.00 13.27 O ATOM 1746 CB LYS A 248 11.778 −22.317 −17.667 1.00 19.07 C ATOM 1747 CG LYS A 248 12.627 −21.784 −18.805 1.00 26.78 C ATOM 1748 CD LYS A 248 12.854 −22.793 −19.913 1.00 32.82 C ATOM 1749 CE LYS A 248 13.947 −22.316 −20.863 1.00 39.04 C ATOM 1750 NZ LYS A 248 13.618 −22.731 −22.257 1.00 41.83 N ATOM 1751 N LYS A 249 12.372 −19.107 −16.783 1.00 13.30 N ATOM 1752 CA LYS A 249 12.130 −17.695 −17.112 1.00 12.64 C ATOM 1753 C LYS A 249 11.705 −17.557 −18.573 1.00 12.82 C ATOM 1754 O LYS A 249 12.215 −18.266 −19.478 1.00 15.20 O ATOM 1755 CB LYS A 249 13.372 −16.843 −16.857 1.00 15.15 C ATOM 1756 CG LYS A 249 13.739 −16.730 −15.383 1.00 18.53 C ATOM 1757 CD LYS A 249 14.765 −15.650 −15.144 1.00 23.21 C ATOM 1758 CE LYS A 249 16.021 −15.843 −15.985 1.00 28.72 C ATOM 1759 NZ LYS A 249 17.151 −14.972 −15.527 1.00 32.25 N ATOM 1760 N ASP A 250 10.799 −16.606 −18.822 1.00 11.89 N ATOM 1761 CA ASP A 250 10.394 −16.187 −20.159 1.00 13.17 C ATOM 1762 C ASP A 250 11.375 −15.094 −20.625 1.00 11.67 C ATOM 1763 O ASP A 250 12.429 −14.870 −20.026 1.00 12.03 O ATOM 1764 CB ASP A 250 8.885 −15.769 −20.138 1.00 13.96 C ATOM 1765 CG ASP A 250 7.931 −16.987 −20.143 1.00 19.37 C ATOM 1766 OD1 ASP A 250 8.025 −17.792 −21.118 1.00 26.27 O ATOM 1767 OD2 ASP A 250 7.095 −17.185 −19.181 1.00 19.97 O ATOM 1768 N ASP A 251 11.067 −14.481 −21.760 1.00 13.72 N ATOM 1769 CA ASP A 251 12.024 −13.587 −22.419 1.00 15.01 C ATOM 1770 C ASP A 251 12.422 −12.403 −21.556 1.00 12.88 C ATOM 1771 O ASP A 251 11.568 −11.796 −20.899 1.00 11.90 O ATOM 1772 CB ASP A 251 11.426 −12.984 −23.699 1.00 18.70 C ATOM 1773 CG ASP A 251 11.122 −13.991 −24.756 1.00 25.89 C ATOM 1774 OD1 ASP A 251 11.732 −15.075 −24.771 1.00 28.98 O ATOM 1775 OD2 ASP A 251 10.247 −13.664 −25.614 1.00 37.49 O ATOM 1776 N ILE A 252 13.693 −12.036 −21.523 1.00 11.96 N ATOM 1777 CA ILE A 252 14.242 −10.917 −20.791 1.00 13.34 C ATOM 1778 C ILE A 252 14.618 −9.845 −21.810 1.00 13.93 C ATOM 1779 O ILE A 252 15.303 −10.110 −22.806 1.00 14.12 O ATOM 1780 CB ILE A 252 15.537 −11.307 −20.086 1.00 14.50 C ATOM 1781 CG1 ILE A 252 15.313 −12.533 −19.202 1.00 16.30 C ATOM 1782 CG2 ILE A 252 16.183 −10.118 −19.383 1.00 15.15 C ATOM 1783 CD1 ILE A 252 14.348 −12.281 −18.135 1.00 16.79 C ATOM 1784 N ARG A 253 14.156 −8.648 −21.570 1.00 11.73 N ATOM 1785 CA ARG A 253 14.450 −7.496 −22.474 1.00 11.81 C ATOM 1786 C ARG A 253 15.264 −6.480 −21.705 1.00 12.05 C ATOM 1787 O ARG A 253 14.859 −6.021 −20.638 1.00 13.99 O ATOM 1788 CB ARG A 253 13.145 −6.872 −22.941 1.00 12.00 C ATOM 1789 CG ARG A 253 13.372 −5.572 −23.719 1.00 11.89 C ATOM 1790 CD ARG A 253 12.112 −5.026 −24.314 1.00 11.33 C ATOM 1791 NE ARG A 253 12.406 −3.751 −24.962 1.00 10.87 N ATOM 1792 CZ ARG A 253 11.584 −3.075 −25.715 1.00 11.37 C ATOM 1793 NH1 ARG A 253 10.342 −3.474 −25.906 1.00 12.79 N ATOM 1794 NH2 ARG A 253 12.004 −1.909 −26.222 1.00 11.06 N ATOM 1795 N GLN A 254 16.411 −6.036 −22.253 1.00 17.74 N ATOM 1796 CA GLN A 254 17.139 −5.016 −21.516 1.00 19.27 C ATOM 1797 C GLN A 254 17.368 −3.729 −22.329 1.00 14.52 C ATOM 1798 O GLN A 254 17.862 −2.808 −21.751 1.00 18.07 O ATOM 1799 CB GLN A 254 18.437 −5.522 −20.835 1.00 24.51 C ATOM 1800 CG GLN A 254 18.099 −6.410 −19.630 1.00 28.36 C ATOM 1801 CD GLN A 254 19.265 −6.712 −18.724 1.00 34.64 C ATOM 1802 OE1 GLN A 254 20.034 −5.819 −18.377 1.00 40.94 O ATOM 1803 NE2 GLN A 254 19.387 −7.987 −18.307 1.00 38.87 N ATOM 1804 N THR A 255 16.832 −3.612 −23.545 1.00 13.49 N ATOM 1805 CA THR A 255 17.015 −2.360 −24.326 1.00 13.10 C ATOM 1806 C THR A 255 15.679 −1.611 −24.419 1.00 11.00 C ATOM 1807 O THR A 255 14.683 −2.086 −24.972 1.00 11.38 O ATOM 1808 CB THR A 255 17.566 −2.620 −25.720 1.00 15.77 C ATOM 1809 OG1 THR A 255 18.878 −3.177 −25.556 1.00 18.94 O ATOM 1810 CG2 THR A 255 17.729 −1.331 −26.508 1.00 15.90 C ATOM 1811 N TYR A 256 15.689 −0.416 −23.830 1.00 9.59 N ATOM 1812 CA TYR A 256 14.481 0.475 −23.789 1.00 9.52 C ATOM 1813 C TYR A 256 14.683 1.862 −24.308 1.00 9.95 C ATOM 1814 O TYR A 256 13.658 2.527 −24.560 1.00 9.89 O ATOM 1815 CB TYR A 256 13.968 0.538 −22.336 1.00 9.58 C ATOM 1816 CG TYR A 256 13.609 −0.811 −21.782 1.00 10.10 C ATOM 1817 CD1 TYR A 256 12.382 −1.405 −22.010 1.00 10.12 C ATOM 1818 CD2 TYR A 256 14.540 −1.523 −21.048 1.00 10.02 C ATOM 1819 CE1 TYR A 256 12.103 −2.666 −21.495 1.00 10.17 C ATOM 1820 CE2 TYR A 256 14.250 −2.801 −20.570 1.00 10.46 C ATOM 1821 CZ TYR A 256 13.022 −3.316 −20.810 1.00 10.46 C ATOM 1822 OH TYR A 256 12.732 −4.594 −20.327 1.00 12.81 O ATOM 1823 N TYR A 257 15.932 2.328 −24.438 1.00 9.59 N ATOM 1824 CA TYR A 257 16.242 3.729 −24.701 1.00 10.02 C ATOM 1825 C TYR A 257 17.174 3.822 −25.897 1.00 11.75 C ATOM 1826 O TYR A 257 17.873 2.878 −26.211 1.00 13.21 O ATOM 1827 CB TYR A 257 16.875 4.377 −23.481 1.00 10.49 C ATOM 1828 CG TYR A 257 15.980 4.324 −22.248 1.00 9.36 C ATOM 1829 CD1 TYR A 257 14.873 5.137 −22.124 1.00 9.77 C ATOM 1830 CD2 TYR A 257 16.244 3.441 −21.226 1.00 8.97 C ATOM 1831 CE1 TYR A 257 14.024 5.066 −21.016 1.00 9.19 C ATOM 1832 CE2 TYR A 257 15.446 3.404 −20.090 1.00 9.18 C ATOM 1833 CZ TYR A 257 14.341 4.214 −19.983 1.00 9.28 C ATOM 1834 OH TYR A 257 13.467 4.202 −18.895 1.00 9.02 O ATOM 1835 N LYS A 258 17.116 4.981 −26.543 1.00 12.59 N ATOM 1836 CA LYS A 258 17.971 5.246 −27.696 1.00 15.57 C ATOM 1837 C LYS A 258 19.435 5.318 −27.308 1.00 20.82 C ATOM 1838 O LYS A 258 19.757 5.768 −26.249 1.00 26.94 O ATOM 1839 CB LYS A 258 17.567 6.592 −28.284 1.00 16.01 C ATOM 1840 CG LYS A 258 16.197 6.561 −28.892 1.00 18.32 C ATOM 1841 CD LYS A 258 15.836 7.805 −30.648 1.00 29.23 C ATOM 1842 CE LYS A 258 14.681 7.482 −30.648 1.00 29.23 C ATOM 1843 NZ LYS A 258 15.151 6.758 −31.872 1.00 35.93 N ATOM 1844 OXT LYS A 258 20.275 4.919 −28.115 1.00 25.57 O TER 1845 LYS A 258 HETATM 1846 CL CL A 301 18.426 0.821 −22.858 1.00 15.14 CL HETATM 1847 CL CL A 302 1.177 4.791 −22.820 1.00 25.04 CL HETATM 1848 CL CL A 303 20.450 −18.960 −7.618 1.00 18.49 CL HETATM 1849 CL CL A 304 −10.599 5.000 −9.695 1.00 30.38 CL HETATM 1850 CL CL A 305 26.057 2.491 −28.651 1.00 39.32 CL HETATM 1851 CL CL A 306 8.900 −24.064 −16.338 1.00 25.76 CL HETATM 1852 CL CL A 307 −3.163 −29.477 −7.877 1.00 13.36 CL HETATM 1853 CL CL A 308 12.791 −0.604 −11.548 1.00 13.72 CL HETATM 1854 CL CL A 309 −8.084 −22.697 −3.752 1.00 17.50 CL HETATM 1855 O HOH A 401 16.090 −11.781 −6.254 1.00 34.86 O HETATM 1856 O HOH A 402 −14.132 −19.872 −8.850 1.00 37.20 O HETATM 1857 O HOH A 403 16.131 17.765 −16.811 1.00 29.85 O HETATM 1858 O HOH A 404 7.357 −4.302 −25.248 1.00 30.64 O HETATM 1859 O HOH A 405 −2.143 −9.749 −26.947 1.00 40.89 O HETATM 1860 O HOH A 406 17.534 5.286 −3.547 1.00 39.90 O HETATM 1861 O HOH A 407 5.756 4.173 −29.801 1.00 17.63 O HETATM 1862 O HOH A 408 3.900 −5.133 1.473 1.00 28.17 O HETATM 1863 O HOH A 409 −14.059 −8.159 4.356 1.00 14.03 O HETATM 1864 O HOH A 410 −15.047 −7.068 8.919 1.00 33.68 O HETATM 1865 O HOH A 411 −17.649 −9.591 1.698 1.00 18.95 O HETATM 1866 O HOH A 412 −2.471 −20.765 7.918 1.00 27.95 O HETATM 1867 O HOH A 413 20.042 5.050 −30.776 1.00 36.76 O HETATM 1868 O HOH A 414 −4.247 −8.999 −28.246 1.00 44.19 O HETATM 1869 O HOH A 415 −10.451 −5.948 9.962 1.00 46.96 O HETATM 1870 O HOH A 416 4.349 16.958 −15.247 1.00 11.74 O HETATM 1871 O HOH A 417 −7.925 −19.390 4.850 1.00 11.14 O HETATM 1872 O HOH A 418 −17.932 −10.827 −0.654 1.00 16.23 O HETATM 1873 O HOH A 419 −1.657 −14.374 −26.782 1.00 31.11 O HETATM 1874 O HOH A 420 −12.601 −14.980 −19.776 1.00 31.01 O HETATM 1875 O HOH A 421 17.299 −9.165 −3.916 1.00 35.26 O HETATM 1876 O HOH A 422 12.658 −12.259 1.194 1.00 23.63 O HETATM 1877 O HOH A 423 −6.393 −8.764 5.259 1.00 15.78 O HETATM 1878 O HOH A 424 19.514 12.920 −24.819 1.00 27.99 O HETATM 1879 O HOH A 425 13.057 −19.812 −3.099 1.00 8.50 O HETATM 1880 O HOH A 426 −8.067 −16.933 3.290 1.00 8.15 O HETATM 1881 O HOH A 427 12.459 17.742 −15.412 1.00 19.96 O HETATM 1882 O HOH A 428 −10.338 −13.828 −21.798 1.00 29.02 O HETATM 1883 O HOH A 429 16.149 9.614 9.485 1.00 40.74 O HETATM 1884 O HOH A 430 −24.880 −8.180 1.476 1.00 17.79 O HETATM 1885 O HOH A 431 3.044 −27.966 −12.055 1.00 27.97 O HETATM 1886 O HOH A 432 5.782 −26.346 −11.225 1.00 26.25 O HETATM 1887 O HOH A 433 −14.955 −18.012 −7.142 1.00 34.75 O HETATM 1888 O HOH A 434 0.268 3.782 −25.515 1.00 36.61 O HETATM 1889 O HOH A 435 10.787 4.686 −32.254 1.00 31.84 O HETATM 1890 O HOH A 436 −17.128 −14.488 −5.668 1.00 21.53 O HETATM 1891 O HOH A 437 5.955 10.113 −24.583 1.00 9.65 O HETATM 1892 O HOH A 438 18.446 1.470 −28.573 1.00 32.18 O HETATM 1893 O HOH A 439 12.098 −16.288 3.363 1.00 18.77 O HETATM 1894 O HOH A 440 11.556 −2.418 −30.217 1.00 29.87 O HETATM 1895 O HOH A 441 −8.728 −21.890 4.530 1.00 16.31 O HETATM 1896 O HOH A 442 −14.103 −17.085 −9.494 1.00 38.37 O HETATM 1897 O HOH A 443 2.260 16.550 −12.547 1.00 24.83 O HETATM 1898 O HOH A 444 14.856 3.774 −31.804 1.00 25.75 O HETATM 1899 O HOH A 445 −1.585 −22.776 −19.019 1.00 24.29 O HETATM 1900 O HOH A 446 −0.018 −6.768 6.537 1.00 25.08 O HETATM 1901 O HOH A 447 5.057 10.814 −22.125 1.00 10.01 O HETATM 1902 O HOH A 448 17.212 5.096 −6.082 1.00 22.49 O HETATM 1903 O HOH A 449 −20.371 −5.149 5.235 1.00 29.36 O HETATM 1904 O HOH A 450 −19.889 −11.126 −4.608 1.00 19.55 O HETATM 1905 O HOH A 451 14.921 4.206 6.848 1.00 43.30 O HETATM 1906 O HOH A 452 13.010 15.114 −21.961 1.00 24.86 O HETATM 1907 O HOH A 453 9.030 −6.394 −22.727 1.00 22.35 O HETATM 1908 O HOH A 454 −20.069 −7.400 −1.215 1.00 13.15 O HETATM 1909 O HOH A 455 3.004 −23.747 2.028 1.00 18.76 O HETATM 1910 O HOH A 456 1.742 −30.056 −7.908 1.00 18.51 O HETATM 1911 O HOH A 457 14.305 6.104 11.325 1.00 30.32 O HETATM 1912 O HOH A 458 8.329 −10.612 −24.974 1.00 27.98 O HETATM 1913 O HOH A 459 3.405 −14.421 5.801 1.00 37.95 O HETATM 1914 O HOH A 460 5.913 −16.955 2.266 1.00 38.68 O HETATM 1915 O HOH A 461 −20.976 −0.544 −11.049 1.00 39.86 O HETATM 1916 O HOH A 462 −1.076 8.801 −17.435 1.00 20.87 O HETATM 1917 O HOH A 463 11.838 0.207 −0.314 1.00 18.96 O HETATM 1918 O HOH A 464 −0.768 11.241 −18.882 1.00 23.55 O HETATM 1919 O HOH A 465 13.821 2.538 0.046 1.00 32.29 O HETATM 1920 O HOH A 466 0.320 −11.514 −24.708 1.00 29.80 O HETATM 1921 O HOH A 467 8.234 −15.650 −2.714 1.00 28.49 O HETATM 1922 O HOH A 468 2.052 12.013 −10.318 1.00 22.42 O HETATM 1923 O HOH A 469 −11.956 −4.817 8.101 1.00 41.49 O HETATM 1924 O HOH A 470 −5.815 −12.211 8.210 1.00 17.11 O HETATM 1925 O HOH A 471 7.757 −3.393 −40.942 1.00 44.06 O HETATM 1926 O HOH A 472 −0.121 −16.246 8.934 1.00 38.49 O HETATM 1927 O HOH A 473 −7.014 −9.111 −24.152 1.00 30.53 O HETATM 1928 O HOH A 474 −14.742 −14.639 −2.806 1.00 10.35 O HETATM 1929 O HOH A 475 2.855 −22.706 −18.778 1.00 26.49 O HETATM 1930 O HOH A 476 16.425 1.807 −6.615 1.00 26.84 O HETATM 1931 O HOH A 477 4.200 21.186 −8.310 1.00 40.53 O HETATM 1932 O HOH A 478 17.460 15.754 −21.085 1.00 41.43 O HETATM 1933 O HOH A 479 14.815 17.167 −21.246 1.00 31.95 O HETATM 1934 O HOH A 480 −4.434 −26.895 −2.388 1.00 23.84 O HETATM 1935 O HOH A 481 −1.593 −21.221 −24.060 1.00 27.38 O HETATM 1936 O HOH A 482 −0.907 7.640 −22.595 1.00 31.78 O HETATM 1937 O HOH A 483 5.915 −21.386 0.447 1.00 23.60 O HETATM 1938 O HOH A 484 2.850 2.090 −38.189 1.00 24.31 O HETATM 1939 O HOH A 485 −22.761 −8.475 −4.929 1.00 28.48 O HETATM 1940 O HOH A 486 −13.100 −24.447 −3.041 1.00 32.17 O HETATM 1941 O HOH A 487 21.426 8.424 −27.700 1.00 38.97 O HETATM 1942 O HOH A 488 3.758 −9.908 7.180 1.00 23.82 O HETATM 1943 O HOH A 489 −3.014 7.153 −18.193 1.00 34.01 O HETATM 1944 O HOH A 490 3.654 −7.695 4.881 1.00 26.55 O HETATM 1945 O HOH A 491 −10.023 −20.608 −4.767 1.00 26.61 O HETATM 1946 O HOH A 492 −8.164 −24.677 −1.392 1.00 32.12 O HETATM 1947 O HOH A 493 3.677 −21.166 4.715 1.00 33.68 O HETATM 1948 O HOH A 494 16.720 −8.351 −25.636 1.00 32.32 O HETATM 1949 O HOH A 495 −1.220 −25.370 0.931 1.00 20.53 O HETATM 1950 O HOH A 496 12.555 −10.054 −25.244 1.00 39.99 O HETATM 1951 O HOH A 497 −8.041 −12.212 10.361 1.00 22.52 O HETATM 1952 O HOH A 498 −23.777 1.060 8.821 1.00 41.39 O HETATM 1953 O HOH A 499 −32.328 −6.495 2.164 1.00 28.68 O HETATM 1954 O HOH A 500 2.089 −20.451 6.924 1.00 36.54 O HETATM 1955 O HOH A 501 22.164 −17.228 −9.757 1.00 39.28 O HETATM 1956 O HOH A 502 15.513 −24.690 −12.348 1.00 36.57 O HETATM 1957 O HOH A 503 6.223 −3.630 0.616 1.00 40.31 O HETATM 1958 O HOH A 504 1.387 3.338 −27.619 1.00 32.60 O HETATM 1959 O HOH A 505 −22.062 −9.319 −2.258 1.00 16.76 O HETATM 1960 O HOH A 506 −8.549 −11.290 −22.586 1.00 26.34 O HETATM 1961 O HOH A 507 13.291 8.908 12.587 1.00 33.89 O HETATM 1962 O HOH A 508 18.726 −21.018 −9.136 1.00 21.89 O HETATM 1963 O HOH A 509 7.374 −8.358 0.294 1.00 44.49 O HETATM 1964 O HOH A 510 15.779 5.574 −36.635 1.00 46.32 O HETATM 1965 O HOH A 511 −3.604 −24.163 1.819 1.00 30.02 O HETATM 1966 O HOH A 512 13.202 −5.289 −2.871 1.00 36.41 O HETATM 1967 O HOH A 513 3.431 −19.605 2.054 1.00 27.16 O HETATM 1968 O HOH A 514 −3.102 5.855 −15.731 1.00 39.11 O HETATM 1969 O HOH A 515 11.646 −6.283 −0.945 1.00 27.17 O HETATM 1970 O HOH A 516 4.785 −12.879 −24.924 1.00 24.03 O HETATM 1971 O HOH A 517 2.641 −10.163 −24.906 1.00 23.34 O HETATM 1972 O HOH A 518 11.103 1.492 −24.919 1.00 11.20 O HETATM 1973 O HOH A 519 12.162 4.904 −25.194 1.00 9.44 O HETATM 1974 O HOH A 520 0.478 −8.400 −24.943 1.00 35.83 O HETATM 1975 O HOH A 521 6.538 −0.371 −24.357 1.00 18.46 O HETATM 1976 O HOH A 522 9.381 −0.359 −23.992 1.00 18.38 O HETATM 1977 O HOH A 523 15.732 −14.001 −22.817 1.00 20.23 O HETATM 1978 O HOH A 524 20.928 9.366 −22.600 1.00 16.64 O HETATM 1979 O HOH A 525 1.085 −18.411 −22.415 1.00 16.82 O HETATM 1980 O HOH A 526 7.054 1.260 −22.273 1.00 11.29 O HETATM 1981 O HOH A 527 −11.381 −9.178 −21.659 1.00 26.83 O HETATM 1982 O HOH A 528 10.316 −8.624 −21.374 1.00 12.36 O HETATM 1983 O HOH A 529 14.905 −15.986 −20.343 1.00 23.12 O HETATM 1984 O HOH A 530 −13.293 −10.568 −20.110 1.00 38.80 O HETATM 1985 O HOH A 531 7.834 −21.301 −17.558 1.00 27.58 O HETATM 1986 O HOH A 532 20.309 2.474 −17.683 1.00 25.74 O HETATM 1987 O HOH A 533 11.154 6.029 −18.590 1.00 8.19 O HETATM 1988 O HOH A 534 5.574 −14.977 −18.103 1.00 10.79 O HETATM 1989 O HOH A 535 8.384 −17.678 −16.952 1.00 11.85 O HETATM 1990 O HOH A 536 18.325 13.947 −17.675 1.00 20.76 O HETATM 1991 O HOH A 537 15.407 −19.958 −16.473 1.00 20.50 O HETATM 1992 O HOH A 538 −13.967 −11.167 −15.840 1.00 29.40 O HETATM 1993 O HOH A 539 8.668 −8.478 −15.192 1.00 8.66 O HETATM 1994 O HOH A 540 −11.095 −5.466 −14.424 1.00 18.33 O HETATM 1995 O HOH A 541 17.506 15.432 −15.536 1.00 26.48 O HETATM 1996 O HOH A 542 −13.565 −6.442 −13.802 1.00 15.00 O HETATM 1997 O HOH A 543 15.491 −0.724 −13.165 1.00 18.09 O HETATM 1998 O HOH A 544 14.965 17.479 −14.028 1.00 23.71 O HETATM 1999 O HOH A 545 19.235 13.396 −12.609 1.00 27.75 O HETATM 2000 O HOH A 546 15.188 −16.785 −11.740 1.00 44.36 O HETATM 2001 O HOH A 547 −15.303 −12.650 −11.594 1.00 19.45 O HETATM 2002 O HOH A 548 −14.371 −9.848 −11.305 1.00 16.30 O HETATM 2003 O HOH A 549 −16.692 −8.385 −11.063 1.00 29.16 O HETATM 2004 O HOH A 550 9.191 13.782 −10.295 1.00 16.83 O HETATM 2005 O HOH A 551 14.678 −6.195 −8.911 1.00 29.32 O HETATM 2006 O HOH A 552 10.503 −0.009 −9.447 1.00 12.11 O HETATM 2007 O HOH A 553 7.407 16.193 −8.454 1.00 33.83 O HETATM 2008 O HOH A 554 14.245 0.558 −8.754 1.00 23.28 O HETATM 2009 O HOH A 555 −8.254 −6.239 −7.389 1.00 8.90 O HETATM 2010 O HOH A 556 15.583 16.745 −6.243 1.00 22.88 O HETATM 2011 O HOH A 557 5.223 −15.225 −4.797 1.00 13.57 O HETATM 2012 O HOH A 558 20.608 11.197 −3.159 1.00 37.10 O HETATM 2013 O HOH A 559 9.497 −8.022 −1.781 1.00 17.57 O HETATM 2014 O HOH A 560 4.663 −16.974 −0.718 1.00 21.71 O HETATM 2015 O HOH A 561 −9.519 −5.033 −0.463 1.00 11.26 O HETATM 2016 O HOH A 562 −3.134 −21.342 0.778 1.00 10.07 O HETATM 2017 O HOH A 563 −11.427 8.200 2.397 1.00 42.57 O HETATM 2018 O HOH A 564 −4.208 −1.220 5.498 1.00 21.15 O HETATM 2019 O HOH A 565 −10.930 −2.843 5.660 1.00 25.10 O HETATM 2020 O HOH A 566 −15.206 0.555 5.175 1.00 26.68 O HETATM 2021 O HOH A 567 3.160 −5.569 −27.349 1.00 28.09 O HETATM 2022 O HOH A 568 22.674 4.700 −26.006 1.00 45.88 O HETATM 2023 O HOH A 569 0.755 −5.048 −26.272 1.00 27.10 O HETATM 2024 O HOH A 570 21.006 −2.108 −24.067 1.00 45.19 O HETATM 2025 O HOH A 571 20.435 2.367 −24.918 1.00 33.27 O HETATM 2026 O HOH A 572 2.033 −13.563 −24.356 1.00 23.32 O HETATM 2027 O HOH A 573 −4.681 −1.091 −23.574 1.00 28.15 O HETATM 2028 O HOH A 574 19.994 6.513 −23.921 1.00 21.49 O HETATM 2029 O HOH A 575 17.869 −10.864 −23.308 1.00 36.78 O HETATM 2030 O HOH A 576 21.427 4.675 −22.385 1.00 31.58 O HETATM 2031 O HOH A 577 1.794 −22.001 −21.357 1.00 35.24 O HETATM 2032 O HOH A 578 20.237 −1.529 −21.318 1.00 32.05 O HETATM 2033 O HOH A 579 −3.981 1.214 −21.799 1.00 26.87 O HETATM 2034 O HOH A 580 9.463 −20.337 −20.300 1.00 41.44 O HETATM 2035 O HOH A 581 18.873 −13.363 −20.506 1.00 42.05 O HETATM 2036 O HOH A 582 19.899 2.707 −20.767 1.00 28.68 O HETATM 2037 O HOH A 583 4.978 −24.363 −19.541 1.00 36.69 O HETATM 2038 O HOH A 584 5.999 −19.625 −19.368 1.00 24.07 O HETATM 2039 O HOH A 585 7.781 −24.160 −18.739 1.00 40.71 O HETATM 2040 O HOH A 586 −10.625 −4.395 −18.233 1.00 31.52 O HETATM 2041 O HOH A 587 −9.993 0.929 −17.580 1.00 50.09 O HETATM 2042 O HOH A 588 −15.658 −9.725 −17.467 1.00 43.12 O HETATM 2043 O HOH A 589 20.083 11.714 −16.656 1.00 35.35 O HETATM 2044 O HOH A 590 8.554 −26.278 −14.624 1.00 31.77 O HETATM 2045 O HOH A 591 −15.050 −14.627 −13.858 1.00 42.58 O HETATM 2046 O HOH A 592 −13.641 −9.289 −13.881 1.00 22.69 O HETATM 2047 O HOH A 593 17.944 −3.324 −11.992 1.00 33.34 O HETATM 2048 O HOH A 594 −12.911 4.662 −11.937 1.00 40.22 O HETATM 2049 O HOH A 595 14.108 −20.749 −11.497 1.00 25.29 O HETATM 2050 O HOH A 596 −17.919 −12.557 −11.453 1.00 41.57 O HETATM 2051 O HOH A 597 12.773 18.165 −11.360 1.00 33.13 O HETATM 2052 O HOH A 598 −19.219 −8.583 −10.346 1.00 36.79 O HETATM 2053 O HOH A 599 9.317 16.534 −10.166 1.00 19.85 O HETATM 2054 O HOH A 600 18.134 17.935 −10.143 1.00 38.52 O HETATM 2055 O HOH A 601 −14.939 −14.502 −9.308 1.00 22.73 O HETATM 2056 O HOH A 602 11.222 15.581 −9.089 1.00 32.45 O HETATM 2057 O HOH A 603 −17.126 −15.004 −8.212 1.00 34.47 O HETATM 2058 O HOH A 604 10.117 14.018 −7.057 1.00 35.85 O HETATM 2059 O HOH A 605 18.565 15.074 −6.285 1.00 23.02 O HETATM 2060 O HOH A 606 4.764 12.709 −2.474 1.00 43.09 O HETATM 2061 O HOH A 607 10.680 2.579 −1.794 1.00 27.19 O HETATM 2062 O HOH A 608 5.446 −13.431 0.552 1.00 30.13 O HETATM 2063 O HOH A 609 −10.498 4.721 3.190 1.00 46.04 O HETATM 2064 O HOH A 610 −21.591 2.307 4.061 1.00 38.24 O HETATM 2065 O HOH A 611 3.493 6.317 4.216 1.00 33.51 O HETATM 2066 O HOH A 612 −5.135 −2.490 −3.550 1.00 19.36 O HETATM 2067 O HOH A 613 −2.436 −0.927 3.082 1.00 33.03 O HETATM 2068 O HOH A 614 0.537 −2.084 −6.675 1.00 12.37 O HETATM 2069 O HOH A 615 2.682 −3.440 −4.739 1.00 23.13 O HETATM 2070 O HOH A 616 5.446 −3.198 −5.100 1.00 20.59 O HETATM 2071 O HOH A 617 4.953 6.718 −11.716 1.00 15.12 O HETATM 2072 O HOH A 618 3.617 3.399 −23.913 1.00 16.70 O HETATM 2073 O HOH A 619 −10.394 1.192 −7.002 1.00 16.39 O HETATM 2074 O HOH A 620 1.520 7.541 −14.539 1.00 19.80 O HETATM 2075 O HOH A 621 5.193 −0.563 −3.872 1.00 20.83 O HETATM 2076 O HOH A 622 5.753 −15.162 −2.041 1.00 23.03 O HETATM 2077 O HOH A 623 −5.455 −4.233 −0.501 1.00 29.89 O HETATM 2078 O HOH A 624 −5.256 1.960 −5.029 1.00 28.68 O HETATM 2079 O HOH A 625 7.689 13.866 1.359 1.00 27.79 O HETATM 2080 O HOH A 626 −2.970 −3.115 1.568 1.00 29.49 O HETATM 2081 O HOH A 627 4.970 −1.481 −0.890 1.00 34.34 O HETATM 2082 O HOH A 628 −3.836 3.749 −8.092 1.00 34.92 O HETATM 2083 O HOH A 629 −4.710 −9.385 9.378 1.00 28.21 O HETATM 2084 O HOH A 630 14.516 −7.872 −11.050 1.00 25.16 O HETATM 2085 O HOH A 631 −1.108 5.740 −14.154 1.00 35.08 O HETATM 2086 O HOH A 632 15.802 14.097 −0.237 1.00 47.68 O HETATM 2087 O HOH A 633 3.927 5.732 −2.638 1.00 31.35 O HETATM 2088 O HOH A 634 0.082 −3.041 3.104 1.00 35.58 O HETATM 2089 O HOH A 635 8.524 −14.329 −23.396 1.00 26.24 O HETATM 2090 O HOH A 636 −7.302 −3.807 −24.128 1.00 30.17 O HETATM 2091 O HOH A 637 −5.390 2.171 −17.173 1.00 28.70 O HETATM 2092 O HOH A 638 −8.032 3.002 −12.999 1.00 46.92 O HETATM 2093 O HOH A 639 −6.613 5.460 −3.014 1.00 33.40 O HETATM 2094 O HOH A 640 2.433 −1.897 −2.309 1.00 31.32 O HETATM 2095 O HOH A 641 0.308 −1.486 −0.028 1.00 34.95 O HETATM 2096 O HOH A 642 11.404 13.663 0.654 1.00 32.09 O HETATM 2097 O HOH A 643 −20.585 −8.163 −8.163 1.00 42.15 O HETATM 2098 O HOH A 644 −5.665 0.364 −3.219 1.00 36.91 O HETATM 2099 O HOH A 645 2.637 1.436 −25.511 1.00 30.90 O HETATM 2100 O HOH A 646 1.410 5.788 −10.687 1.00 37.16 O HETATM 2101 O HOH A 647 17.547 0.214 −11.281 1.00 39.34 O HETATM 2102 O HOH A 648 −2.919 −4.742 −28.133 1.00 40.96 O HETATM 2103 O HOH A 649 3.290 3.520 −4.923 1.00 20.97 O HETATM 2104 O HOH A 650 7.434 19.750 −8.876 1.00 25.30 O HETATM 2105 O HOH A 651 −4.452 2.471 −10.917 1.00 23.59 O HETATM 2106 O HOH A 652 1.227 4.726 −6.191 1.00 24.61 O HETATM 2107 O HOH A 653 2.902 0.896 −4.431 1.00 25.62 O HETATM 2108 O HOH A 654 −0.670 0.995 −7.854 1.00 26.21 O HETATM 2109 O HOH A 655 −2.948 2.308 −13.755 1.00 29.76 O HETATM 2110 O HOH A 656 −10.710 −3.677 −16.328 1.00 32.90 O HETATM 2111 O HOH A 657 −2.819 1.447 11.702 1.00 39.16 O HETATM 2112 O HOH A 658 −5.415 2.009 19.376 1.00 31.72 O MASTER 318 0 9 14 4 0 12 6 2076 1 0 18

TABLE 3 Amino Acid Sequences SEQ ID NO: 1 (Amino acid sequence of full length AtlE of S. aureus)         10         20         30         40         50 MKKNFKLRIS TLLLIVILVV FAVLLIVNET KLFKNDVNYS FDEAVSMQQG         60         70         80         90        100 KGIVQTKEED GKFVEANNNE IAKAMTISHK DNDMKYMDIT EKVPMSESEV        110        120        130        140        150 NQLLKGKGIL ENRGKVFLEA QEKYEVNVIY LVSHALVETG NGKSELAKGI        160        170        180        190        200 KDGKKRYYNF FGIGAFDSSA VRSGKSYAEK EQWTSPDKAI IGGAKFIRNE        210        220        230        240        250 YFENNQLNLY QMRWNPENPA QHQYASDIRW ADKIAKLMDK SYKQFGIKKD DIRQTYYK SEQ ID NO: 2 (SAV2307_Atl) NDVNYSFDEA VSMQQGKGIV QTKEEDGKFV EANNNEIAKA MTISHKDNDM KYMDITEKVP MSESEVNQLL KGKGILENRG KVFLEAQEKY EVNVIYLVSH ALVETGNGKS ELAKGIKDGK KRYYNFFGIG AFDSSAVRSG KSYAEKEQWT SPDKAIIGGA KFIRNEYFEN NQLNLYQMRW NPENPAQHQY ASDIRWADKI AKLMDKSYKQ FGIKKDDIRQ TYYK SEQ ID NO: 3 (SAV0909_Atl) SPYTFKQALD KQMARGNPKK SNAWGWANAT RAQTSSAMNV KRIWESNTQC YQMLNLGKYQ GVSVSALNKI LKGKGTLNNQ GKAFAEACKK HNINEIYLIA HAFLESGYGT SNFANGKDGV YNYFGIGAYD NNPNYAMTFA RNKGWTSPAK AIMGGASFVR KDYINKGQNT LYRIRWNPKN PATHQYATAI EWCQHQKNTI ASLKQFIILK GIYFTRDKYK SEQ ID NO: 4 (SAV1775_Atl) EKLPVTLNDA AKKQINNYT SQKVSNKKNDA WRDASATQTK SAMDSGTFID NEKQKYQFLN LSKYQGIDKN RIKCMLVDR PTLLKHTDDFA KAAKDKHVNE VYLIAHAFLE SGAVTSNFAN GVEIDGDKVY NYYGVGALD KDPIKTAAEFA KNHGWDTPEK AISGGADFVH KHFLSSTDQN TLYSMRWNPK NPGEHQYAT AIKWCESNQTI IADFLKNMKT EGKYFKYFVK KSKHLNK SEQ ID NO: 5 (SAV2644_Atl) PASDDKANQK SKSKDNQSMS DSALDSILDQ YSDDAKKTQK DYASQGKKDK NEKSNTQNPQ LPTQDELKHK SKPAQSFNND VNQKDTRATS MFETDPSISK NDDSGQFNVV DSKDTRQFVK SIADDAHRIG QDNDIYASVM IAQAILESDS VRSAFANSPN HNLFGIKGAF EGNSVPFNTL EADGNKLYSI NAGFRKYPTT KESLKDYSDL VKKGIDGNRT IYKPTWKSKA DSYKDATSHL SSTYATDPNY AKKLNSIIKH YQLFQFDDER MPDLHDK SEQ ID NO: 6 (SAV1052_AtlA) PQTGMTLNQV AQIKANLQYK PSVQRVPDQ WTDDAFKDVK HYADTKRLAQ DPALKYQFLR LDQPQNIKID KITSFFETDP VISKQAAAA NKIAQMNGIY EVVMIAQAIL ESDSVTSQFA NSADVVNNKV VTNSNTKEHN VVPFATYEN DPLKEYIKNA KQAGYDTVSE SLVDYSDLVG KSIVKAGNNT IYKPTWKSAH DGTHQYKTA VDHLNINYAI IKGYADKINE VGKYFDIPQFD SEQ ID NO: 7 (LytB_SP) NPFLFKDLTE ATNYSAEELD KVFSLLNINN SLLENKGATF KEAEEHYHIN ALYLLAHSAL ESNWGRSKIA KDKNNFFGIT TFDDVDKGIL GATKWIKENY IDRGRTFLGN KASGMNVEYA SDPYWGEKIA SVMMKINEKL GGKD SEQ ID NO: 8 (LMO) QQTFINSIST QAMDLCKHYN LYPSVMIAQS ALESNWGRSE LDKAPNNNLF GIKGSYNGKS VTMKTWEYSD SKGWYQINAN FAKYPDHKES LEDNTKKLKE GPSWDSSYYK GAWRENAKTY KDATAWLQGR YATDNTYGSK LNTVISSYNL TQYD SEQ ID NO: 9 (FLgJ) AQAFVDATWP QAAKLAQSLG LPAHFMIAQS ALESNWGRSQ IRNKDGTPSN NLFNIKGGSN WTGKVVEART VKVRVERFRA YDDYEQSFQD YTDLVGESPR YAKVAGKTDG HAFARALQEG GYATDPSYGS KLNRVINGNA LRQRLMASAA SARGLE SEQ ID NO: 10 (ACOD) AARMDKYKSF INNVAKSHVL DPAVIAATIS RESRAGNVIF NTTPPGWGDN YNGFGLMQVD KRYHEPRGAW NSEEHFDQAT GILVEFIQLI QKKFPSWSTE VQLKGAIAAY NTGDGRVESY ESVDSRTTGK DYSNDVVARA QWYKLNGF SEQ ID NO: 11 (HLYZ) KVFERCDLAR TLKRLGMDGH RGISLANWMC LIKWESRYNT RITNYNAGDR STDFGIMQVN SRYWCNDGKT PGAVNACHLS CSALLQDNIA DHVACAKRVV RDPQGIRAWV AWRNDCQNRD VRQYVQGCGV 

1. A crystal of autolysin E (AtlE) of Staphylococcus aureus (S. aureus), or a crystallizable fragment of AtlE.
 2. The crystal according to claim 1, wherein AtlE or a crystallizable fragment thereof contains glutamic acid, or an equivalent thereof, as the catalytic residue.
 3. The crystal according to claim 1 or 2, wherein the crystallizable fragment of AtlE is a N-terminal truncation of AtlE, in particular lacking the N-terminal transmembrane region.
 4. The crystal according to any one of claims 1 to 3, wherein the crystal is a co-crystal of AtlE or its crystallizable fragment and a substrate and/or a candidate compound.
 5. The crystal according to any one of claims 1 to 4, wherein the AtlE crystal or its crystallizable fragment is characterized by (a) the space group symmetry P2₁2₁2₁ and (b) the unit cell dimensions of a=46.6 Å±1-2 Å, b=69.9 Å±1-2 Å and c=73.3 Å±1-2 Å, in particular a=46.6 Å±1 Å, b=69.9 Å±1 Å and c=73.3 Å±1 Å, with α=90°, β=90° and γ=90°.
 6. A method for producing a crystal of autolysin E (AtlE) of Staphylococcus aureus (S. aureus) or a crystallizable fragment thereof, said method comprising the steps of: (a) preparing a solution of said AtlE in a crystallization buffer, preferably containing 2 M NaCl and 2 M (NH₄)₂SO₄, and (b) crystallizing said AtlE by vapor diffusion.
 7. A method for screening a binding compound or inhibitor of the N-acetylglucosaminidase activity of autolysin E (AtlE) of Staphylococcus aureus (S. aureus), said method comprising the steps of: (a) providing a solution of, said AtlE or a crystallizable fragment thereof, (b) contacting at least one candidate compound with the AtlE in said solution, (c) preparing crystals of said AtlE, and (d) identifying a binding compound of said AtlE.
 8. The method of claim 7, wherein AtlE or a crystallizable fragment thereof contains glutamic acid, or an equivalent thereof, as the catalytic residue.
 9. The method of claim 7 or 8, wherein the crystallizable fragment of AtlE is a N-terminal truncation of AtlE, in particular lacking the N-terminal transmembrane region, preferably comprising the amino acid sequence of SEQ ID NO:
 1. 10. The method of any of claims 7 to 9, wherein said AtlE comprises the amino acid sequence of the glucosaminidase domain of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, or SEQ ID NO:
 6. 11. The method according to any of claims 7 to 10, wherein in step (d) the binding of the candidate compound to the active site is determined.
 12. The method according to claim 11, wherein the active site is characterized by the regions of SEQ ID NO: 1 from methionine 47 (M) to glutamic acid 65 (E), from leucine 136 (L) to glycine 140 (G), from asparagine 159 (N) to glutamic acid 181 (E), from phenylalanine 196 (F) to asparagine 204 (N) and/or from proline 219 (P) to lysine 233 (K), and in particular the catalytic glutamic acid (E) at position 138, and optionally further by an aspartic acid (D) at position 167, an phenyl alanine (F) at position 224, an aspartic acid (D) at position 227 and/or a tyrosine (Y) at position
 201. 13. Use of the crystal as defined in any one of claims 1 to 5 for obtaining atomic spatial relationship data.
 14. Use of the crystal according to claim 13 for screening, identifying, designing, or optimizing a binding compound, in particular a naturally occurring or synthetic ligand or inhibitor, of said AtlE.
 15. Use of the crystal structure of AtlE of S. aureus for in silico screening of the ability of a candidate compound to bind to said AtlE and/or any of the S. aureus autolysins comprising the amino acid sequence selected from SEQ ID NO: 2, 3, 4, or 5, in particular to bind to the active site of the said AtlE and/or the active site(s) of said other autolysins.
 16. A Kit containing a solution of autolysin E (AtlE) of Staphylococcus aureus (S. aureus), or a crystallizable fragment of AtlE, and a crystallization buffer containing 2 M NaCl and 2 M (NH₄)₂SO₄. 